Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 3,306,434 G→A 9.6% L428L (CTG→TTG)  deaD ← ATP‑dependent RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,306,4340GA 9.6% 157.7 / 5.7 52L428L (CTG→TTG) deaDATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base G (23/24);  new base A (2/3);  total (25/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTTGCTCAGC  >  W3110S.gb/3306365‑3306498
                                                                     |                                                                
cACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGt                                                                 >  1:429863/1‑71 (MQ=255)
 aCGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGt                                                                 <  1:1648174/70‑1 (MQ=255)
 aCGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTg                                                                <  1:3980338/71‑1 (MQ=255)
          aCGCATCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCa                                                       <  1:1757376/71‑1 (MQ=255)
             cATCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTg                                                                <  1:1412045/59‑1 (MQ=255)
              aTCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGt                                                   <  1:1105951/71‑1 (MQ=255)
                 ggCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGttt                                                 <  1:2996063/70‑1 (MQ=255)
                 ggCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGttt                                                 >  1:112298/1‑70 (MQ=255)
                 ggCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTc                                                >  1:374572/1‑71 (MQ=255)
                   cgcATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCga                                              <  1:1422997/71‑1 (MQ=255)
                    gcATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCgag                                             <  1:3178621/71‑1 (MQ=255)
                    gcATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCgag                                             <  1:2466394/71‑1 (MQ=255)
                     cATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCgaga                                            <  1:2384717/71‑1 (MQ=255)
                      aTCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGAt                                           <  1:1909155/71‑1 (MQ=255)
                        cTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATc                                          >  1:3943836/1‑70 (MQ=255)
                        cTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATc                                          >  1:1653875/1‑70 (MQ=255)
                         tGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGc                                                               <  1:3176515/48‑1 (MQ=255)
                         tGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGtt                                                  >  1:1004324/1‑61 (MQ=255)
                         tGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATc                                          >  1:2790810/1‑69 (MQ=255)
                         tGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGACGCAGCCAGAGTTTc                                                >  1:1895819/1‑63 (MQ=255)
                          ggCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCGAGATCCa                                        >  1:1315018/1‑70 (MQ=255)
                          ggCGGTACGATCAGAGTACGTTCAACCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCg                                               >  1:2101751/1‑63 (MQ=255)
                           gCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATcc                                         <  1:2575368/68‑1 (MQ=255)
                             ggTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGttt                                                 <  1:140410/58‑1 (MQ=255)
                                  gATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCagag                                                    >  1:2126283/1‑50 (MQ=255)
                                  gATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGttt                                                 >  1:2263921/1‑53 (MQ=255)
                                   aTCAGAGTACGTTGACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGtt                                                  >  1:1996515/1‑51 (MQ=255)
                                   aTCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCagag                                                    <  1:3219214/49‑1 (MQ=255)
                                     cAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCtt                            >  1:1889282/1‑71 (MQ=255)
                                     cAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCtt                            >  1:566783/1‑71 (MQ=255)
                                     cAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCtt                            >  1:3638528/1‑71 (MQ=255)
                                     cAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCtt                            >  1:1999866/1‑71 (MQ=255)
                                     cAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCt                             >  1:2702853/1‑70 (MQ=255)
                                                  cccTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTca                          >  1:1958522/1‑60 (MQ=255)
                                                  cccTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTg               <  1:2090610/71‑1 (MQ=255)
                                                       tgCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGAt                                           <  1:2654824/38‑1 (MQ=255)
                                                       tgCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAAttt          >  1:613140/1‑71 (MQ=255)
                                                       tgCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAAtt           <  1:2302594/70‑1 (MQ=255)
                                                       tgCCATTTTCAGCAATGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAAt            <  1:3261104/69‑1 (MQ=255)
                                                         ccATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTTAAtttt         <  1:4127155/70‑1 (MQ=255)
                                                          cATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTTGc       >  1:4177974/1‑71 (MQ=255)
                                                          cATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTTGc       >  1:1062318/1‑71 (MQ=255)
                                                           aTTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTg               <  1:3731547/62‑1 (MQ=255)
                                                            ttttCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCAc                               <  1:2235207/45‑1 (MQ=255)
                                                            ttttCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCAc                               <  1:117174/45‑1 (MQ=255)
                                                            ttttCACCAGTGCCGCAGCCAGAGTTTCCAGATcc                                         <  1:10311/35‑1 (MQ=37)
                                                             tttCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAg                                       >  1:143385/1‑36 (MQ=255)
                                                             tttCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCATTCGGCTGAAttt          >  1:3843767/1‑65 (MQ=255)
                                                             tttCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTATTCACCTTCAGCAGTCGGCTGAATTTTGCt      >  1:4102680/1‑69 (MQ=255)
                                                              ttCAGCAATGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTcag                         <  1:2909394/49‑1 (MQ=255)
                                                              ttCAGCAATGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTT‑CTcag   <  1:1406810/70‑1 (MQ=255)
                                                               tCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTTGCTcag   <  1:3291400/70‑1 (MQ=255)
                                                                cagcagTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTTGCTcagc  <  1:1239146/70‑1 (MQ=255)
                                                                     |                                                                
CACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTTGCTCAGC  >  W3110S.gb/3306365‑3306498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑