| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,332,615 | T→A | 10.2% | intergenic (‑25/+102) | yhbE ← / ← rpmA | conserved inner membrane protein/50S ribosomal subunit protein L27 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,332,615 | 0 | T | A | 10.2% | 155.8 / 10.2 | 59 | intergenic (‑25/+102) | yhbE/rpmA | conserved inner membrane protein/50S ribosomal subunit protein L27 |
| Reads supporting (aligned to +/- strand): ref base T (27/26); new base A (2/4); total (29/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
TGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCG > W3110S.gb/3332548‑3332684 | tggtgAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGTATTTg < 1:1256846/69‑1 (MQ=255)tggtgAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCg > 1:3113756/1‑71 (MQ=255)tggtgAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCg < 1:2345907/71‑1 (MQ=255)tggtgAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTAGCg < 1:3096505/71‑1 (MQ=255) aGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGAtt < 1:486623/70‑1 (MQ=255) aGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTa < 1:2940664/71‑1 (MQ=255) aGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTa < 1:2377492/71‑1 (MQ=255) aGCGCCAAAAGAATGCCAATGCCTGCCTGCAGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGAtt > 1:181026/1‑70 (MQ=255) gcgcCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCg > 1:1096682/1‑65 (MQ=255) cgcCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAAc > 1:4239466/1‑71 (MQ=255) cAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCg < 1:535730/61‑1 (MQ=255) cAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCg < 1:2993129/61‑1 (MQ=255) cAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTc > 1:909873/1‑71 (MQ=255) cAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTc > 1:368075/1‑71 (MQ=255) cAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTc < 1:2280380/71‑1 (MQ=255) cAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTAGCGGATTAACGt < 1:3935389/70‑1 (MQ=255) aaaaGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCg > 1:3440685/1‑60 (MQ=255) aaaGAATGCCAATGCCTGCCTTCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCg < 1:610092/59‑1 (MQ=255) aaaGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGa < 1:2895123/61‑1 (MQ=255) aaaGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCg > 1:3661858/1‑70 (MQ=255) aaaGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTAGCGGATTAACGTCg > 1:1685255/1‑70 (MQ=255) aaGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGc > 1:3337297/1‑70 (MQ=255) aatgccaatgccTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCa > 1:1712114/1‑71 (MQ=255) aatgccTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCa > 1:2660691/1‑71 (MQ=255) aatgccTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCa < 1:1849985/71‑1 (MQ=255) atgccTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCAt > 1:2031929/1‑71 (MQ=255) tgcctgccTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTg < 1:3211375/45‑1 (MQ=255) gcctgcctGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGc > 1:578543/1‑45 (MQ=255) cctgcctgCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGc > 1:644329/1‑44 (MQ=255) cctgcctgCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGc > 1:1209174/1‑44 (MQ=255) cctgcctgCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCa > 1:554700/1‑58 (MQ=255) cctgcctgCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCAtttt < 1:3273870/71‑1 (MQ=255) cctgcctgCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCAtttt > 1:3019187/1‑71 (MQ=255) cctgcTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCa < 1:3805439/54‑1 (MQ=255) cctgcTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTa < 1:639239/69‑1 (MQ=255) ctgctgCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCg < 1:4094786/71‑1 (MQ=255) tgctgcTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCAt < 1:3828803/56‑1 (MQ=255) tgctTCATACCGTATTCCCTGCAAAGCGCATTTGCg < 1:2784629/36‑1 (MQ=255) tgctTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATgcgc < 1:335849/57‑1 (MQ=255) gctTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCAttttt > 1:3686004/1‑62 (MQ=255) gctTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCAttttt > 1:3817654/1‑62 (MQ=255) gctTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTa < 1:644553/63‑1 (MQ=255) ctTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCAcc < 1:1987655/49‑1 (MQ=255) cATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCa > 1:1232480/1‑47 (MQ=255) cATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGaa > 1:162433/1‑71 (MQ=255) aTACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGaa < 1:1208901/70‑1 (MQ=255) tACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCAttttt < 1:1465627/56‑1 (MQ=255) tACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTAc > 1:3491998/1‑58 (MQ=255) cGTATTCCCTGCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTaa < 1:2383746/71‑1 (MQ=255) gTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTaa > 1:1486006/1‑70 (MQ=255) tCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGt > 1:2229597/1‑54 (MQ=255) cTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTaa < 1:3121940/63‑1 (MQ=255) gCAAAGCGCATTTGCGGATTAACGTCGCACCATgcgc > 1:872783/1‑37 (MQ=255) cAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCA‑TTTTACCGGTTATCGAATGTAAAAAGCCCCGc < 1:1325304/69‑1 (MQ=255) aaGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAAc < 1:1929903/71‑1 (MQ=255) aGCGCATTTGCGGATTAACGTCGCACCATTCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACg < 1:4216414/71‑1 (MQ=255) aGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGt > 1:829581/1‑55 (MQ=255) cgcATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGcc > 1:3669599/1‑61 (MQ=255) gcATTTGCGGATTAACGTCGCACCATGCGCATTATTACCGGTTATCGAATGTAAAAAGCCCCGCAACgtgt > 1:3678928/1‑71 (MQ=255) tGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCg < 1:3197283/70‑1 (MQ=255) tGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCg < 1:2782429/70‑1 (MQ=255) tGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCg < 1:1910826/70‑1 (MQ=255) | TGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCG > W3110S.gb/3332548‑3332684 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |