| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,459,280 | T→C | 10.2% | E15G (GAA→GGA) | secE ← | preprotein translocase membrane subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,459,280 | 0 | T | C | 10.2% | 154.9 / 9.9 | 59 | E15G (GAA→GGA) | secE | preprotein translocase membrane subunit |
| Reads supporting (aligned to +/- strand): ref base T (30/23); new base C (2/4); total (32/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.98e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01 | |||||||||||
TCGCGATAAAGATAGTTGCCGACAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAAACCAACCTGTCAGAAGTTTTCTAC > W3110S.gb/3459211‑3459349 | tCGCGATAAAGATAGTTGCCGACAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTTc > 1:1946/1‑71 (MQ=255) aTAAAGATAGTTGCCGACAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAgg < 1:1717606/70‑1 (MQ=255) tAAAGATAGTTGCCGACAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGcc > 1:555207/1‑71 (MQ=255) aaaGATAGTTGCCGACAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGcc > 1:2060834/1‑70 (MQ=255) aaaGATAGTTGCCGACAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCcg > 1:2548613/1‑71 (MQ=255) aaGATAGTTGCCGACAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTTc < 1:2796282/63‑1 (MQ=255) gATAGTTGCCGACAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCa < 1:1802880/63‑1 (MQ=255) tAGTTGCCGACAATGGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGcc > 1:1122826/1‑70 (MQ=255) gCCGACAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCtt < 1:2668787/70‑1 (MQ=255) cGACAATCGCCACCAGGAGCAATGCCACAACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCt > 1:661323/1‑71 (MQ=255) gACAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCtt < 1:1679342/71‑1 (MQ=255) aCAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCtt < 1:4198473/70‑1 (MQ=255) aCAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCtt > 1:2082801/1‑70 (MQ=255) cAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCtt > 1:3453249/1‑69 (MQ=255) cAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCtt > 1:248540/1‑69 (MQ=255) cAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGa < 1:384720/71‑1 (MQ=255) tCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGc < 1:3205012/60‑1 (MQ=255) tCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCt > 1:62221/1‑71 (MQ=255) cGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGc < 1:881339/59‑1 (MQ=255) ccaccaGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCg > 1:3393180/1‑71 (MQ=255) caccaGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCtt > 1:4075479/1‑68 (MQ=255) caccaGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCgg < 1:2209972/71‑1 (MQ=255) caccaGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCgg < 1:3719060/71‑1 (MQ=255) ccaGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTcc < 1:530360/58‑1 (MQ=255) aGGAGCAATGCCACCACAACGACCGACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTAt > 1:3317972/1‑70 (MQ=255) aGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCa < 1:3953255/40‑1 (MQ=255) ggAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAg > 1:693501/1‑60 (MQ=255) aGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCg > 1:2875511/1‑70 (MQ=255) gCAATGCCACCACAACGACCCACTTCATCGCTTCCAg < 1:753265/37‑1 (MQ=255) gCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTc > 1:1978194/1‑68 (MQ=255) cAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCa > 1:1950929/1‑70 (MQ=255) tGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCa < 1:3601694/71‑1 (MQ=255) ccaccaCAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCTCa > 1:4254777/1‑65 (MQ=255) ccaccaCAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATa < 1:715289/71‑1 (MQ=255) ccaccaCAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATa < 1:3080148/71‑1 (MQ=255) ccaccaCAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATa < 1:1802275/71‑1 (MQ=255) accacAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATaaa > 1:3990353/1‑71 (MQ=255) acaACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTc < 1:4179393/50‑1 (MQ=255) acaACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAaacc > 1:2975555/1‑70 (MQ=255) caACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAg > 1:3073899/1‑45 (MQ=255) caACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAaacaac > 1:133142/1‑68 (MQ=255) aCGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAaaccaacc > 1:266920/1‑71 (MQ=255) aCGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTACTTGAGCTTCGGt > 1:4244893/1‑50 (MQ=255) gACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAAACCAACCTg > 1:1880577/1‑71 (MQ=255) aCTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTAtt > 1:1809816/1‑46 (MQ=255) aCTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAAACCAACCTGTCAGa < 1:2443669/71‑1 (MQ=255) cTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTa < 1:2937870/43‑1 (MQ=255) cTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCAt < 1:2982277/54‑1 (MQ=255) tCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAAACCAACCTGTCAGAAGt > 1:1838715/1‑71 (MQ=255) cATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAAACCAACCTGTc > 1:104358/1‑64 (MQ=255) cATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAAACCAACCTGTc > 1:81955/1‑64 (MQ=255) aTCGCTTCCAGGCCGCTCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAAACCAACCTGTCAGAAGtt < 1:4028705/70‑1 (MQ=255) aTCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAAACCAACCTGTCAGAAGtt < 1:4039423/70‑1 (MQ=255) aTCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAAACCAACCTGTCAGAAGtt < 1:871599/70‑1 (MQ=255) tCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAAACCAACCTGTCAGAAGtttt < 1:815316/71‑1 (MQ=255) tCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAAACCAACCTGTCAGAAGttt > 1:2910282/1‑70 (MQ=255) cGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAAACCAACCTGTCAGAAGTTTTc > 1:1025652/1‑71 (MQ=255) gCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAAACCAACCTGTCAGAAGTTTTc < 1:224248/70‑1 (MQ=255) gCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAAACCAACCTGTCAGAAGTTTTc > 1:3528378/1‑70 (MQ=255) tCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAAACCAACCTGTCAGAAGTTTTCTAc > 1:407608/1‑70 (MQ=255) | TCGCGATAAAGATAGTTGCCGACAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAAACCAACCTGTCAGAAGTTTTCTAC > W3110S.gb/3459211‑3459349 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |