| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,497,165 | G→A | 10.8% | A65T (GCG→ACG) | fsaB → | fructose‑6‑phosphate aldolase 2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,497,165 | 0 | G | A | 10.8% | 136.5 / 5.4 | 46 | A65T (GCG→ACG) | fsaB | fructose‑6‑phosphate aldolase 2 |
| Reads supporting (aligned to +/- strand): ref base G (23/18); new base A (3/2); total (26/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGTGCTGCCGCGTCTGCAAAAAGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGACGCTATTCCGGGTATTGTGGTGAAAATCCCGGT > W3110S.gb/3497095‑3497235 | aGTGCTGCCGCGTCTGCAAAAAGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGAcg > 1:1089710/1‑71 (MQ=255)aGTGCTGCCGCGTCTGCAAAAAGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGAcg > 1:3381667/1‑71 (MQ=255) gctgcCGCGTCTGCAAAAAGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGAcgc > 1:3801753/1‑69 (MQ=255) ctgcCGCGTCTGCAAAAAGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGAcgcg > 1:3236935/1‑69 (MQ=255) ctgcCGCGTCTGCAAAAAGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACgcgc < 1:880556/70‑1 (MQ=255) ccGCGTCTGCAAAAAGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAggg > 1:1426321/1‑71 (MQ=255) ccGCGTCTGCAAAAAGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACACGCAgg < 1:2321030/70‑1 (MQ=255) gTCTGCAAAAAGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGAcgcg > 1:318640/1‑62 (MQ=255) tGCAAAAAGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGAtggtg > 1:1895175/1‑71 (MQ=255) aaaaaGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCATGGGAtggtgg < 1:70825/69‑1 (MQ=255) aaaaaGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGAtggtgg < 1:3810924/69‑1 (MQ=255) aaaaaGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGgaa < 1:2317414/71‑1 (MQ=255) aaaGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAgg < 1:1147766/58‑1 (MQ=255) aaaGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGAtg < 1:1652981/63‑1 (MQ=255) aaaGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGgaaga < 1:3269085/71‑1 (MQ=255) aGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGgaagaa > 1:1831150/1‑70 (MQ=255) gCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGAcgcg < 1:1841436/51‑1 (MQ=255) gCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAgaagc < 1:3144252/71‑1 (MQ=255) aTTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGAcgc < 1:3110117/47‑1 (MQ=255) aTTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGAcgc < 1:1012020/47‑1 (MQ=255) aTTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGAcgc < 1:3606348/47‑1 (MQ=255) ttGGTTATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAgaagc < 1:122841/67‑1 (MQ=255) ttGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAgaagcgaag > 1:4075628/1‑71 (MQ=255) ttGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAgaagcgaag > 1:2821485/1‑71 (MQ=255) gTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAgaagcgaa < 1:1590649/67‑1 (MQ=255) gatgaGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAGAAGCGAAGCGCCt > 1:2089674/1‑71 (MQ=255) atgaGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAGAAGCGAAGCGCCTg > 1:755376/1‑71 (MQ=255) atgaGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAGAAGCGAAGCGCCTg > 1:571212/1‑71 (MQ=255) tgaGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAGAAGCGAAGCGCCTgc > 1:1402213/1‑71 (MQ=255) tgggCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGTGAtggtgg < 1:1431051/50‑1 (MQ=255) aGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGAtggtg > 1:425707/1‑50 (MQ=255) aGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGgaag > 1:429334/1‑54 (MQ=255) aGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGGCAGAAGCGAAGCGCCTgcgc < 1:3502646/71‑1 (MQ=255) gggCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGcgcg < 1:2463085/71‑1 (MQ=255) aTTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGACGc < 1:2327922/71‑1 (MQ=255) ttCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGACGc > 1:2922103/1‑70 (MQ=255) cTGTTTGCTCAGACCATGAGCCGCGACACGCAGGGGATGGTGGAAGAAGCGAAgcgc > 1:2576805/1‑57 (MQ=255) tGTTTTCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGACGCTAtt > 1:3138620/1‑71 (MQ=255) tGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGACGCTAtt > 1:1186928/1‑71 (MQ=255) tGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGACGCTAt > 1:3153153/1‑70 (MQ=255) cTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGga < 1:2392634/37‑1 (MQ=255) cAGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAGAAGCGAAGCGCCTgcgc < 1:3168681/54‑1 (MQ=255) aGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAGAAGCGAAGCGCCTgcgc > 1:3164945/1‑53 (MQ=255) aGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGACGCTATTCCGGGTAtt > 1:2995690/1‑71 (MQ=255) aGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGACGCTATTCCGGGTAtt < 1:3943004/71‑1 (MQ=255) gACCATGAGCCGCGACGCGCAGGGGATGGTGGAAgaagcgaag > 1:4172383/1‑43 (MQ=255) ccATGAGCCGCGACGCGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGACGCTATTCCGGGTATtgtg > 1:3455454/1‑71 (MQ=255) ccATGAGCCGCGACGCGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGACGCTATTCCGGGTATtgt < 1:943613/70‑1 (MQ=255) cATGAGCCGCGACGCGCAGGGGATGGTGGAAgaagcgaagccc > 1:4033642/1‑41 (MQ=255) aTGAGCCGCGACGCGCAGGGGATGGTGGAAgaagcg < 1:1395996/36‑1 (MQ=255) tGAGCCGCGACGCGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGACGCTATTCCGGGTATTgtggt > 1:20430/1‑70 (MQ=255) tGAGCCGCGACGCGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGACGCTATTCCGGGTATTgtggt > 1:1141318/1‑70 (MQ=255) tGAGCCGCGACACGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGACGCTATTCCGGGt > 1:2866291/1‑62 (MQ=255) tGAGCCGCGACACGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGACGCTATTCCGGGTATTgtggt > 1:1031164/1‑70 (MQ=255) cgcgACACGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGACGCTATTCCGGGTATtgtg < 1:1436635/63‑1 (MQ=255) gcgACGCGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGACGCTATTCCGGGTATTGTGGTGa > 1:425226/1‑66 (MQ=255) gcgcAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGACGCTATTCCGGGTATTGTGGTGAAAATCCCGGt < 1:2104332/71‑1 (MQ=255) | AGTGCTGCCGCGTCTGCAAAAAGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGACGCTATTCCGGGTATTGTGGTGAAAATCCCGGT > W3110S.gb/3497095‑3497235 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |