| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,516,285 | T→C | 6.9% | intergenic (+20/‑47) | hslU → / → menA | molecular chaperone and ATPase component of HslUV protease/1,4‑dihydroxy‑2‑naphthoate octaprenyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,516,285 | 0 | T | C | 6.9% | 156.6 / 4.1 | 58 | intergenic (+20/‑47) | hslU/menA | molecular chaperone and ATPase component of HslUV protease/1,4‑dihydroxy‑2‑naphthoate octaprenyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (24/30); new base C (2/2); total (26/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
GATGCGTTGGTGGCAGATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGTATTATGACTGAACAACAAATTAGCCGA > W3110S.gb/3516221‑3516355 | gATGCGTTGGTGGCAGATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTtcatca < 1:1437602/67‑1 (MQ=255)gATGCGTTGGTGGCAGATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTtcacca < 1:2978020/67‑1 (MQ=255) tGCGTTGGTGGCAGATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGttt < 1:215914/71‑1 (MQ=255) cGTTGGTGGCAGATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGa > 1:438653/1‑71 (MQ=255) tggtggCAGATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATg > 1:2890528/1‑70 (MQ=255) gCAGATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCt < 1:3146136/70‑1 (MQ=255) gCAGATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCt > 1:3898787/1‑70 (MQ=255) gCAGATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCACCATTGTTTGATGGGGCt > 1:79438/1‑70 (MQ=255) cAGATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGa < 1:2951828/71‑1 (MQ=255) cAGATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCACCATTGTTTGATGGGGCTGa < 1:3737196/71‑1 (MQ=255) aGATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGttt < 1:3637369/59‑1 (MQ=255) aTGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGa < 1:2281288/59‑1 (MQ=255) aTGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGa < 1:2412586/59‑1 (MQ=255) aTGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGa < 1:2201308/68‑1 (MQ=255) aTGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAg < 1:4146224/71‑1 (MQ=255) tGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTtcat > 1:4095946/1‑48 (MQ=255) gAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATg > 1:1908225/1‑59 (MQ=255) gAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCACCATTGTTTg > 1:2367583/1‑56 (MQ=255) aaGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGcc > 1:4074496/1‑71 (MQ=255) aGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGccc > 1:416518/1‑71 (MQ=255) aGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGccc < 1:1675723/71‑1 (MQ=255) aGATCTGAGCCGTTTTATCCTATAATAGCGTTCAATCATTTTCATCATTGtt > 1:434557/1‑52 (MQ=255) gATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGt < 1:3501227/50‑1 (MQ=255) gATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGccc > 1:880921/1‑70 (MQ=255) cTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTcatcat < 1:2900485/44‑1 (MQ=255) tGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCt < 1:2308331/57‑1 (MQ=255) aGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGa > 1:2777919/1‑57 (MQ=255) aGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCACCATTGTTTGATGGGGCTGAAAGGCCCCAtttt < 1:2007189/70‑1 (MQ=255) aGCCGTTTTATCCTATAATCGCGTTCAATCAATTTCATCATTGTTTGATGGGGCTGAAAGGCCCCAttt < 1:188169/69‑1 (MQ=255) ccGTTTTATCCTATAATCGCGTTCAATCATTTtcatc < 1:164558/37‑1 (MQ=255) ttttATCCTATAATCGCGTTCAATCATTTtcatca < 1:2059367/35‑1 (MQ=255) ttttATCCTATAATCGCGTTCAATCATTTTCATCATTGttt < 1:1086917/41‑1 (MQ=255) ttttATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAgg < 1:572518/56‑1 (MQ=255) ttATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGa > 1:2350633/1‑41 (MQ=255) ttATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGa > 1:1331317/1‑41 (MQ=255) ttATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTg > 1:4172576/1‑68 (MQ=255) ttATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGgcg > 1:4052078/1‑71 (MQ=255) ttATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGgcg > 1:3514465/1‑71 (MQ=255) ttATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGgcg > 1:897329/1‑71 (MQ=255) ttATCCGATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGgcg > 1:554997/1‑71 (MQ=255) cTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGa < 1:2177963/45‑1 (MQ=255) tataATCGCGTTCAATCATTTTCATCATTGTTTGa > 1:2806529/1‑35 (MQ=255) taATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTg < 1:3255290/41‑1 (MQ=255) taATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTg < 1:3680459/41‑1 (MQ=255) taATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGtattat > 1:3572236/1‑71 (MQ=255) tCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGTATTATGAc > 1:1518040/1‑71 (MQ=255) gcgTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGTATTATGAc > 1:2990999/1‑69 (MQ=255) cgTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGTATTATGACTGa < 1:3484980/71‑1 (MQ=255) ttCAATCATTTTCATCATTGTTTGATGGGGCTGaa < 1:3861214/35‑1 (MQ=255) cAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCAttttt < 1:680663/46‑1 (MQ=255) cAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGTATTATGACTGaacaa > 1:1390418/1‑71 (MQ=255) cAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGTATTATGACTGaacaa > 1:58832/1‑71 (MQ=255) aaTCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGTATTATGACTGaacaa > 1:2036275/1‑70 (MQ=255) aaTCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGTATTATGACTGaacaa < 1:2965603/70‑1 (MQ=255) tCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGtatta > 1:3105436/1‑57 (MQ=255) tCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGTATTATGa < 1:4175133/60‑1 (MQ=255) tCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGTATTATGACTGAAcaacaa < 1:2501052/71‑1 (MQ=255) aTTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGTATTATGACTGAACAACaaa < 1:2345647/70‑1 (MQ=255) aTTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGTATTATGACTGAACAACaaa < 1:1806381/70‑1 (MQ=255) aTTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGTATTATGACTGAACAACAAAt < 1:2253187/71‑1 (MQ=255) aTTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGTATTATGACTGAACAACAAAt < 1:2747931/71‑1 (MQ=255) aTTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGTATTATGACTGAACAACAAAt < 1:664353/71‑1 (MQ=255) ttttCATCATTGTTTGATGGGGCGGAAAGGCCCCATTTTTATTGGCGCGTATTATGACTGAACAACAAAtt < 1:1538468/71‑1 (MQ=255) ttCATCATTGTTTGATGGGGCTGAAAGGCCCCAtttt < 1:3810744/37‑1 (MQ=255) atcatTGTTTGATGGGGCTGAAAGGCCTCATTTTTATTGGCGCGTATTATGACTGAACAACAAATTAGcc > 1:2018330/1‑70 (MQ=255) atcatTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGTATTATGACTGAACAACAAATTAGCCg < 1:1890408/71‑1 (MQ=255) tcatTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGTATTATGACTGAACAACAAATTAGCCGa < 1:381101/71‑1 (MQ=255) | GATGCGTTGGTGGCAGATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGTATTATGACTGAACAACAAATTAGCCGA > W3110S.gb/3516221‑3516355 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |