| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,639,730 | T→C | 10.6% | K222R (AAA→AGA) | xerC ← | site‑specific tyrosine recombinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,639,730 | 0 | T | C | 10.6% | 171.1 / 11.7 | 66 | K222R (AAA→AGA) | xerC | site‑specific tyrosine recombinase |
| Reads supporting (aligned to +/- strand): ref base T (28/31); new base C (4/3); total (32/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGAGTGACGTAATTTATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGCGTCGTCTTCGCTACCA > W3110S.gb/3639660‑3639798 | cGAGTGACGTAATTTATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGtt < 1:1601252/71‑1 (MQ=255)cGAGTGACGTAATTTATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGtt > 1:2048812/1‑71 (MQ=255) gAGTGACGTAATTTATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGttt < 1:988673/71‑1 (MQ=255) gAGTGACGTAATTTATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGttt < 1:2071013/71‑1 (MQ=255) aGTGACGTAATTTATGAGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTc < 1:279887/71‑1 (MQ=255) gTGACGTAATTTATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCt > 1:2844512/1‑71 (MQ=255) tGACGTAATTTATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTg > 1:1594197/1‑71 (MQ=255) aCGTAATTTATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGca > 1:1987175/1‑71 (MQ=255) aCGTAATTTATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTGca > 1:4142793/1‑71 (MQ=255) gTAATTTATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGcaca > 1:19870/1‑71 (MQ=255) cGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTAcgcgcg < 1:767229/70‑1 (MQ=255) cGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTAcgcgcg < 1:2194307/70‑1 (MQ=255) cGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTGCACATTAcgcgcg < 1:2088540/70‑1 (MQ=255) ggATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGga < 1:3304459/71‑1 (MQ=255) gATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGgag < 1:3238763/71‑1 (MQ=255) gATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGgag < 1:1127600/71‑1 (MQ=255) tGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGgaga > 1:3822456/1‑70 (MQ=255) aaCGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATAc > 1:1401723/1‑71 (MQ=255) aCGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACAtt > 1:804923/1‑56 (MQ=255) cGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGc < 1:3773521/71‑1 (MQ=255) gTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTAcgcgc < 1:2099829/60‑1 (MQ=255) gTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTAcgcgc < 1:4237074/60‑1 (MQ=255) gTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCt < 1:4100942/71‑1 (MQ=255) gTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCt < 1:3828569/71‑1 (MQ=255) gATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTg > 1:3027935/1‑71 (MQ=255) attattCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGtt < 1:2954809/41‑1 (MQ=255) attattCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGtt < 1:2819549/41‑1 (MQ=255) attattCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGc < 1:3276968/71‑1 (MQ=255) ttattaAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGcc > 1:1836417/1‑71 (MQ=255) ttattCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTc < 1:1873199/42‑1 (MQ=255) attCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGga > 1:297447/1‑57 (MQ=255) attCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTGCACATTACGCGCGGAGATACGc < 1:349157/64‑1 (MQ=255) tCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTAcgcgc < 1:4106970/52‑1 (MQ=255) tCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGc < 1:1120880/62‑1 (MQ=255) tCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTGCACATTACGCGCGGAGATACGCt < 1:3268873/63‑1 (MQ=255) cAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTa > 1:4016263/1‑46 (MQ=255) cAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGtt > 1:3728810/1‑71 (MQ=255) cagccTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGttt > 1:1758278/4‑71 (MQ=255) aGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGgaga < 1:916748/55‑1 (MQ=255) aGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTGCACATTACGCGCGGAGATAc > 1:190319/1‑58 (MQ=255) gCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTc < 1:12241/71‑1 (MQ=255) cccTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCg < 1:3583909/71‑1 (MQ=255) ccTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGgaga < 1:4092035/52‑1 (MQ=255) cTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACg > 1:3863714/1‑55 (MQ=255) cTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGa > 1:1020706/1‑70 (MQ=255) ttGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTAc < 1:2975579/41‑1 (MQ=255) tGTTTTATGCCCCATTCGGCAAAGAGTTTCTGCACATTAcgcgcg > 1:934447/1‑45 (MQ=255) gTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTAcg > 1:3348034/1‑40 (MQ=255) gTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATa > 1:1693968/1‑50 (MQ=255) gTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACa < 1:3311935/69‑1 (MQ=255) tttATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGaa > 1:881009/1‑70 (MQ=255) ttATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCa > 1:2093842/1‑57 (MQ=255) ttATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGa > 1:2551833/1‑64 (MQ=255) aTGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGgaga < 1:2909961/43‑1 (MQ=255) aTGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGaaaaa < 1:1102562/70‑1 (MQ=255) ccATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAgcgc > 1:1317240/1‑69 (MQ=255) cATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtc < 1:4099446/71‑1 (MQ=255) ttCGGCAAAGCGTTTCTGCACATTACGCGCGgaga < 1:3596202/35‑1 (MQ=255) ttCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt > 1:3676668/1‑71 (MQ=255) cGGCAAAGCGTTTCTGTACATTACGCGCGGAGATACGCATGCCCAGTTTCGACAGAAAAAGCGCGTCGTCt > 1:1392189/1‑71 (MQ=255) cGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGCgtcgtc < 1:1710886/70‑1 (MQ=255) cGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGCGTCGTCt > 1:3905818/1‑71 (MQ=255) cGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGCGTCGTCt > 1:3623330/1‑71 (MQ=255) cGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGCGTCGTCt > 1:863541/1‑71 (MQ=255) cGGCAAAGCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAgcg > 1:961794/1‑63 (MQ=255) ggCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGCGTCGGCt < 1:2608646/70‑1 (MQ=255) ggCAAAGCGTTTCAGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtc < 1:3886799/66‑1 (MQ=255) aaaGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGCGTCGTCTTCGc > 1:460071/1‑71 (MQ=255) aaaGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGCGTCGTCTTCGc < 1:4280940/71‑1 (MQ=255) aaaGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGCGTCGTCTTCGc > 1:2885359/1‑71 (MQ=255) aaGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGCGTCGTCTTCGCt < 1:3169891/71‑1 (MQ=255) gTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGCGTCGTCTTCGCTACCa < 1:3668421/71‑1 (MQ=255) | CGAGTGACGTAATTTATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGCGTCGTCTTCGCTACCA > W3110S.gb/3639660‑3639798 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |