Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 3,739,327 C→T 10.5% V195I (GTT→ATT)  yieH ← predicted hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,739,3270CT10.5% 231.1 / 13.9 76V195I (GTT→ATT) yieHpredicted hydrolase
Reads supporting (aligned to +/- strand):  ref base C (37/31);  new base T (4/4);  total (41/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01

TCCCAACCACGCGCTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCGTCGATACCAGATTGTG  >  W3110S.gb/3739257‑3739395
                                                                      |                                                                    
tCCCAACCACGCGCTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAAc                                                                      >  1:1080611/1‑71 (MQ=255)
  ccAACCACGCGCTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACg                                                                     <  1:1344185/70‑1 (MQ=255)
  ccAACCACGCGCTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGa                                                                    >  1:2279532/1‑71 (MQ=255)
  ccAACCACGCGCTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGa                                                                    <  1:3104897/71‑1 (MQ=255)
    aaCCACGCGCTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATc                                                                  >  1:436153/1‑71 (MQ=255)
    aaCCACGCGCTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATc                                                                  <  1:1286412/71‑1 (MQ=255)
     aCCACGCGCTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACg                                                                     <  1:3063567/67‑1 (MQ=255)
      ccACGCGCTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCgg                                                                >  1:2710863/1‑71 (MQ=255)
              tttCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATc                                                                  >  1:3418210/1‑61 (MQ=255)
               ttCCACAGTTCAGGTAACTGCGAAAGATGGGTAAACGTGGTGACTTTCGGGTGAACGATCGCCTTATtgt                                                        >  1:2809372/1‑70 (MQ=255)
                tCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATtgt                                                        <  1:1628272/69‑1 (MQ=255)
                tCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGc                                                      >  1:1328187/1‑71 (MQ=255)
                tCCACAGTTCAGGTAACTGCGAAAGATGGCTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATtgt                                                        <  1:3892724/69‑1 (MQ=255)
                 ccACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGc                                                      <  1:3597103/70‑1 (MQ=255)
                  cacaGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATtgt                                                        <  1:940369/67‑1 (MQ=255)
                  cacaGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGct                                                     <  1:1250248/70‑2 (MQ=255)
                  cacaGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCgg                                                    >  1:1818166/1‑71 (MQ=255)
                   acaGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCggg                                                   >  1:775665/1‑71 (MQ=255)
                     aGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTc                                                 >  1:4090531/1‑71 (MQ=255)
                      gTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCg                                                <  1:4177048/71‑1 (MQ=255)
                        tCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTa                                                            >  1:2432446/1‑57 (MQ=255)
                          aGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGgcgc                                            <  1:4002027/71‑1 (MQ=255)
                           ggTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATtg                                                         >  1:1891226/1‑57 (MQ=255)
                            gTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAg                                          <  1:1615698/71‑1 (MQ=255)
                                cTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGaagaag                                       >  1:3131240/1‑70 (MQ=255)
                                cTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAg                                       >  1:3248057/1‑70 (MQ=255)
                                 tGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGAt                                                                   >  1:3939394/1‑41 (MQ=255)
                                 tGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAg                                       >  1:1263175/1‑69 (MQ=255)
                                  gCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATtgtg                                                       >  1:3445472/1‑52 (MQ=255)
                                   cGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGaa                                        >  1:236783/1‑66 (MQ=255)
                                   cGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAg                                    >  1:1200618/1‑70 (MQ=255)
                                    gAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGaa                                  <  1:2034056/71‑1 (MQ=255)
                                     aaaGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGgc                                              >  1:3983385/1‑58 (MQ=255)
                                     aaaGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAac                                 <  1:3460522/71‑1 (MQ=255)
                                     aaaGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGaa                                  >  1:3058557/1‑70 (MQ=255)
                                      aaGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAaca                                <  1:2723321/71‑1 (MQ=255)
                                         aTGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACtt                             >  1:219232/1‑71 (MQ=255)
                                          tGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGt                                      <  1:932233/61‑1 (MQ=255)
                                          tGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTc                            <  1:2124600/71‑1 (MQ=255)
                                           gggTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTc                            >  1:4187962/1‑70 (MQ=255)
                                           gggTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTc                            >  1:647685/1‑70 (MQ=255)
                                           gggTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTc                            >  1:3359485/1‑70 (MQ=255)
                                             gTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTa                                                            <  1:2856293/36‑1 (MQ=255)
                                             gTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGgcg                                             <  1:2672745/51‑1 (MQ=255)
                                             gTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCAt                         <  1:1806415/71‑1 (MQ=255)
                                             gTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCAt                         <  1:3725739/71‑1 (MQ=255)
                                               aaaGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAg                                    <  1:4238077/58‑1 (MQ=255)
                                               aaaGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAg                                    <  1:1272105/58‑1 (MQ=255)
                                               aaaGGTGGTGACTTTCGGGTGAACGATCGGATTATTGTGCGGGTCg                                                >  1:1311359/1‑46 (MQ=255)
                                                aaGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTa                                     >  1:1475592/1‑56 (MQ=255)
                                                aaGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAg                                    <  1:2535423/57‑1 (MQ=255)
                                                aaGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATAcc                      >  1:4031669/1‑71 (MQ=255)
                                                aaGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATAc                       <  1:438405/70‑1 (MQ=255)
                                                aaGGTGGTGACTATCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGaa                                  >  1:2544448/1‑59 (MQ=255)
                                                 aGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATAc                       <  1:901402/69‑1 (MQ=255)
                                                   gtggtgACTTTCGGGTGAATGATCGGCTTATTGTGCg                                                     <  1:3870755/37‑1 (MQ=255)
                                                   gtggtgACTTTCGGGTGAACGATCGGCTTATtgtg                                                       <  1:1983670/35‑1 (MQ=255)
                                                    tggtgACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGgcgc                                            <  1:3467509/45‑1 (MQ=255)
                                                     ggtgACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCGt                 >  1:3180637/1‑71 (MQ=255)
                                                      gtgACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGgcg                                             <  1:1300510/42‑1 (MQ=255)
                                                       tgACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCGTCg               <  1:423712/71‑1 (MQ=255)
                                                        gACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATa                        >  1:2994414/1‑61 (MQ=255)
                                                        gACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCGTCGa              >  1:1079582/1‑71 (MQ=255)
                                                         aCTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCGTCGAt             <  1:867991/71‑1 (MQ=255)
                                                          cTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAg                                          >  1:360385/1‑41 (MQ=255)
                                                          cTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTa                                     >  1:3701132/1‑46 (MQ=255)
                                                           tttCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCGTCGa              <  1:16408/68‑1 (MQ=255)
                                                           tttCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCGTCGATAc           >  1:3578474/1‑71 (MQ=255)
                                                             tCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATAc                       >  1:1714045/1‑57 (MQ=255)
                                                             tCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCGTCGATAc           >  1:2064665/1‑69 (MQ=255)
                                                              cGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCGTCGATAc           <  1:593880/68‑1 (MQ=255)
                                                                   gAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCGTCGATACCAGATtgt   >  1:3733589/1‑71 (MQ=255)
                                                                   gAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCGTCGATACCAGATtgt   >  1:1381172/1‑71 (MQ=255)
                                                                    aaCGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGa                                   >  1:1063536/1‑38 (MQ=255)
                                                                    aaCGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCGTCGATACCAGATtgtg  >  1:2581372/1‑71 (MQ=255)
                                                                     aCGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAg                                    <  1:2664184/36‑1 (MQ=255)
                                                                     aCGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGaa                                  >  1:1236761/1‑38 (MQ=255)
                                                                      cGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCa                          >  1:3272868/1‑45 (MQ=255)
                                                                      |                                                                    
TCCCAACCACGCGCTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCGTCGATACCAGATTGTG  >  W3110S.gb/3739257‑3739395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑