| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,832,784 | T→C | 7.3% | C156R (TGC→CGC) | rfaQ → | lipopolysaccharide core biosynthesis protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,832,784 | 0 | T | C | 7.3% | 181.2 / 6.7 | 68 | C156R (TGC→CGC) | rfaQ | lipopolysaccharide core biosynthesis protein |
| Reads supporting (aligned to +/- strand): ref base T (23/40); new base C (3/2); total (26/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.63e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
TTTATCGGTCCTTGAGCCATTAGGTATTACCGATTTCTACACCGACACAACAATGAGTTACGCCGAAGATTGCTGGAAGAAGATGCGCCGGGAATTAGATGCCCTGGGCGTAAAAGATCATTATGTTGTCATCCAACCGA > W3110S.gb/3832714‑3832853 | tttATCGGTCCTTGAGCCATTAGGTATTACCGATTTCTACACCGACACAACAATGAGTTACGCCGAAGAtt > 1:861933/1‑71 (MQ=255)tttATCGGTCCTTGAGCCATTAGGTATTACCGATTTCTACACCGACACAACAATGAGTTACGCCGAAGAtt < 1:4021299/71‑1 (MQ=255) tATCGGTCCTTGAGCCATTAGGTATTACCGATTTCTACACCGACACAACAATGAGTTACGCCGAAGATTGc > 1:79320/1‑71 (MQ=255) tATCGGTCCTTGAGCCATTAGGTATTACCGATTTCTACACCGACACAACAATGAGTTACGCCGAAGATCGc > 1:37217/1‑71 (MQ=255) ccTTGAGCCATTAGGTATTACCGATTTCTACACCGACACAACAATGAGTTACGCCGAAGATCGCTGgaag > 1:724913/1‑70 (MQ=255) cTTGAGCCATTAGGTATTACCGATTTCTACACCGACACAACAATGAGTTACGCCGAAGATTGCTGgaagaa < 1:352502/71‑1 (MQ=255) cTTGAGCCATTAGGTATTACCGATTTCTACACCGACACAACAATGAGTTACGCCGAAGATTGCTGgaagaa < 1:4131249/71‑1 (MQ=255) cTTGAGCCATTAGGTATTACCGATTTCTACACCGACACAACAATGAGTTACGCCGAAGATTGCTGgaaga < 1:477428/70‑1 (MQ=255) cTTGAGCCATTAGGTATTACCGATTTCTACACCGACACAACAATGAGTTACGCCGAAGATTGCTGgaag > 1:3439562/1‑69 (MQ=255) gAGCCATTAGGTATTACCGATTTCTACACCGACACAACAATGAGTTACGCCGAAGATTGCTGGaagaag < 1:1125466/69‑1 (MQ=255) gAGCCATTAGGTATTACCGATTTCTACACCGACACAACAATGAGTTACGCCGAAGATTGCTGGaagaag < 1:1248163/69‑1 (MQ=255) gAGCCATTAGGTATTACCGATTTCTACACCGACACAACAATGAGTTACGCCGAAGATTGCTGGaagaag < 1:4220315/69‑1 (MQ=255) gAGCCATTAGGTATTACCGATTTCTACACCGACACAACAATGAGTTACGCCGAAGATTGCTGGAagaaga < 1:250394/70‑1 (MQ=255) gAGCCATTAGGTATTACCGATTTCTACACCGACACAACAATGAGTTACGCCGAAGATTGCTGGAagaaga < 1:4019098/70‑1 (MQ=255) gAGCCATTAGGTATTACCGATTTCTACACCGACACAACAATGAGTTACGCCGAAGATTGCTGGAagaaga < 1:1151777/70‑1 (MQ=255) gAGCCATTAGGTATTACCGATTTCTACACCGACACAACAATGAGTTACGCCGAAGATTGCTGGAAGAAGAt > 1:4036878/1‑71 (MQ=255) aGCCATTAGGTATTACCGATTTCTACACCGACACAACAATGAGTTACGCCGAAGATTGCTGGAAGAAGAt < 1:2185573/70‑1 (MQ=255) aTTAGGTATTACCGATTTCTACACCGACACAACAATGAGTTACGCCGAAGATTGCTGGAAGAAGATGCGcc < 1:2118419/71‑1 (MQ=255) ttAGGTATTACCGATTTCTACACCGACACAACAATGAGTTACGCCGAAGATTGCTGGAAGAAGATGCGCCg > 1:1307951/1‑71 (MQ=255) aGGTATTACCGATTTCTACACCGACACAACAATGAGTTACGCCGAAGATTGCTGGAAGAAGATGCGCCgg > 1:4241173/1‑70 (MQ=255) aGGTATTACCGATTTCTACACCGACACAACAATGAGTTACGCCGAAGATTGCTGGAAGAAGATGCGCCgg > 1:4245961/1‑70 (MQ=255) aGGTATTACCGATTTCTACACCGACACAACAATGAGTTACGCCGAAGATTGCTGGAAGAAGATGCGCCgg > 1:1502274/1‑70 (MQ=255) aGGTATTACCGATTTCTACACCGACACAACAATGAGTTACGCCGAAGATTGCTGGAAGAAGATGCGCCgg > 1:608185/1‑70 (MQ=255) gTATTACCGATTTCTACACCGACACAACAATGAGTTACGCCGAAGATTGCTGGAAGAAGATGCGCCggg < 1:400676/69‑1 (MQ=255) tATTACCGATTTCTACACCGACACAACAATGAGTTACGCCGAAGATTGCTTGAAGAAGATGCGCCGGGAAt < 1:1126971/71‑1 (MQ=255) tATTACCGATTTCTACACCGACACAACAATGAGTTACGCCGAAGATTGCTGGAAGAAGATGCGCCGGGAAt < 1:1525354/71‑1 (MQ=255) tATTACCGATTTCTACACCGACACAACAATGAGTTACGCCGAAGATTGCTGGAAGAAGATGCGCCGGGAAt < 1:251905/71‑1 (MQ=255) ttACCGATTTCTACACCGACACAACAATGAGTTACGCCGAAGATTGCTGGAAGAAGATGCGCCGGGAAtt < 1:3936974/70‑1 (MQ=255) ttACCGATTTCTACACCGACACAACAATGAGTTACGCCGAAGATTGCTGGAAGAAGATGCGCCGGGAAt < 1:3669358/69‑1 (MQ=255) ttACCGATTTCTACACCGACACAACAATGAGTTACGCCGAAGATCGCTGGAAGAAGATGCGCCGGGa < 1:56967/67‑1 (MQ=255) aCCGATTTCTACACCGACACAACAATGAGTTACGCCGAAGATTGCTGGaagaag > 1:2183654/1‑54 (MQ=255) cGATTTCTACACCGACACAACAATGAGTTACGCCGAAGATTGCTGgaa > 1:2701807/1‑48 (MQ=255) cGATTTCTACACCGACACAACAATGAGTTACGCCGAAGATTGCTGgaa > 1:1281907/1‑48 (MQ=255) cGATTTCTACACCGACACAACAATGAGTTACGCCGAAGATTGCTGGAAGAAGAt > 1:1574445/1‑54 (MQ=255) cGAATTCTACACCGACACAACAATGAGTTACGCAGAAGATTGCTGgaa > 1:1561898/1‑48 (MQ=255) aTTTCTACACCGACACAACAATGAGTTACGCCGAAGATTGCTGGAAGAAGATGCGCCGGGAATTAGa > 1:2856863/1‑67 (MQ=255) ttCTACACCGACACAACAATGAGTTACGCCGAAGATTGCTGGAagaaga > 1:2834769/1‑49 (MQ=255) ttCTACACCGACACAACAATGAGTTACGCCGAAGATTGCTGGAAGAAGATGCGCCg > 1:3194012/1‑56 (MQ=255) ttCTACACCGACACAACAATGAGTTACGCCGAAGATTGCTGGAAGAAGATGCGCCGGGAAt > 1:2840564/1‑61 (MQ=255) ttCTACACCGACACAACAATGAG‑TACGCCGAAGATTGCTGGAAGAAGATGCGCCGGGAATTAGATGCCCt < 1:4071895/70‑1 (MQ=255) tCTACACCGACACAACAATGAGTTACGCCGAAGATTGCTGGAAGAAGATGCGCCGGGaa > 1:3669987/1‑59 (MQ=255) cacCGAAACAACAATGAGTTACGCCGAAGATTGCTGGAAGAAGATGCGCAGGGAATTAGATGCCCTGGGc < 1:1568072/70‑1 (MQ=255) acCGACACAACAATGAGTTACGCCGAAGATTGCTGGAAGAAGATGCGCCgg > 1:1214036/1‑51 (MQ=255) acCGACACAACAATGAGTTACGCCGAAGATTGCTGGAAGAAGATGCGCCGGGAAtt < 1:2364069/56‑1 (MQ=255) ccGACACAACAATGAGTTACGCCGAAGATTGCTGgaagaa < 1:4245559/40‑1 (MQ=255) cGACACAACAATGAGTTACGCCGAAGATTGCTGGAAGAAGATGCGCCGGGAATTAGATGCCCTGGGCGTaa < 1:3531673/71‑1 (MQ=255) cGACACAACAAAGAGTTACGCCGAAGATTGCTGGAAGAAGATGCGCCGGGAATTAGATGCCCTGGGCGTaa < 1:476567/71‑1 (MQ=255) gACACAACAATGAGTTACGCCGAAGATTGCTGGAAGAAGATGCGCCGGGAATTAGATGCCCTGGGCGTaaa < 1:2102668/71‑1 (MQ=255) acaacaATGAGTTACGCCGAAGATTGCTGgaagaa < 1:48470/35‑1 (MQ=255) acaacaATGAGTTACGCCGAAGATTGCTGGAAGAAGATGCGCCGGGAATTa < 1:3320266/51‑1 (MQ=255) caacaaTGAGTTACGCCGAAGATTGCTGGAAGAAGATGCGCCGGGAATTa < 1:2414775/50‑1 (MQ=255) caacaaTGAGTTACGCCGAAGATTGCTGGAAGAAGATGCGCCGGGAATTa < 1:2657154/50‑1 (MQ=255) caacaaTGAGTTACGCCGAAGATTGCTGGAAGAAGATGCGCCGGGAATTAGATGCCCTGGGCGTAAAAGAt < 1:1267464/71‑1 (MQ=255) acaaTGAGTTACGCCGAAGATTGCTGGAAGAAGATGCGCCGGGAATTAGATGCCCTGGGCGTAAAAGATCa < 1:2899058/71‑1 (MQ=255) caaTGAGTTACGCCGAAGATTGCTGGAAGAAGATGCGCCGGGAATTAGATGCCCTGGGCGTAAAAGATCAt < 1:3489435/71‑1 (MQ=255) caaTGAGTTACGCCGAAGATTGCTGGAAGAAGATGCGCCGGGAATTAGATGCCCTGGGCGTAAAAGATCAt > 1:542606/1‑71 (MQ=255) caaTGAGTTACGCCGAAGATTGCTGGAAGAAGATGCCCCGGGAATTAGATGCCCTGGGCGTAAAAGATCAt > 1:1659197/1‑71 (MQ=255) aaTGAGTTACGCCGAAGATTGCTGGAAGAAGATGCGCCGGGAATTAGGTGCCCTGGGCGTAAAAg < 1:4267999/65‑1 (MQ=255) aaTGAGTTACGCCGAAGATTGCTGGAAGAAGATGCGCCGGGAATTAGATGCCCTGGGCGTAAAAg < 1:806475/65‑1 (MQ=255) aaTGAGTTACGCCGAAGATTGCTGGAAGAAGATGCGCCGGGAATTAGATGCCCTGGGCGTAAAAGATCAt > 1:776357/1‑70 (MQ=255) aGTTACGCCGAAGATTGCTGGAAGAAGATGCGCCGGGAATTa < 1:430946/42‑1 (MQ=255) aGTTACGCCGAAGATTGCTGGAAGAAGATGCGCCGGGAATTAGATGCCCTGGGCGTAAAAGATGATTAtg < 1:546895/70‑1 (MQ=255) ttACGCCGAAGATTGCTGGAAGAAGATGCGCCGGGAATTAGa < 1:3901314/42‑1 (MQ=255) ttACGCCGAAGATTGCTGGAAGAAGATGCGCCGGGAATTAGATGCCCt < 1:789152/48‑1 (MQ=255) gCCGAAGATTGCTGGAAGAAGATGCGCCGGGAATTAGATGCCCTGGGCGTAAAAGATCATTATGTTGTCAt < 1:3191065/71‑1 (MQ=255) cGAAGATTGCTGGAAGAAGATGCGCCGGGAATTAGATGCCCTGGGCGTAAAAGATCATTATGTTGTCATcc < 1:11946/71‑1 (MQ=255) gAAGATCGCTGGAAGAAGATGCGCCGGGAATTAGATGCCCTGGGCGTaaaa > 1:1163799/1‑51 (MQ=255) aaGATTGCTGGAAGAAGATGCGCCGGGAATTAGATGCCCTGGGCGTaaaa > 1:2400701/1‑50 (MQ=255) gATTGCTGGAAGAAGATGCGCCGGGAATTAGATGCCCTGGGCGTAAAAGATCATTATGTTGTCATCCAAcc < 1:3424258/71‑1 (MQ=255) gATCGCTGGAAGAAGATGCGCCGGGAATTAGATGCCCTGGGCGTAAAAGATCATTATGTTGTCATCCAAcc < 1:428931/71‑1 (MQ=255) tGCTGGAAGAAGATGCGCCGGGAATTAGATGCCCTGGGCGTAAAAGATCATTATGTTGTCATCCaa < 1:2251612/66‑1 (MQ=255) tGCTGGAAGAAGATGCGCCGGGAATTAGATGCCCTGGGCGTAAAAGATCATTATGTTGTCATCCAACCGa > 1:1722706/1‑70 (MQ=255) tGCTGGAAGAAGATGCGCCGGGAATTAGATGCCCTGGGCGTAAAAGATCATTATGTTGTCATCCAACCGa < 1:2512275/70‑1 (MQ=255) | TTTATCGGTCCTTGAGCCATTAGGTATTACCGATTTCTACACCGACACAACAATGAGTTACGCCGAAGATTGCTGGAAGAAGATGCGCCGGGAATTAGATGCCCTGGGCGTAAAAGATCATTATGTTGTCATCCAACCGA > W3110S.gb/3832714‑3832853 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |