| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,836,867 | A→G | 8.7% | K206K (AAA→AAG) | rfaB → | UDP‑D‑galactose:(glucosyl)lipopolysaccharide‑1, 6‑D‑galactosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,836,867 | 0 | A | G | 8.7% | 118.2 / 4.5 | 46 | K206K (AAA→AAG) | rfaB | UDP‑D‑galactose:(glucosyl)lipopolysaccharide‑1, 6‑D‑galactosyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (17/25); new base G (2/2); total (19/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01 | |||||||||||
CCCGAGGAAGGTGAAACGGCAACGTTTATTTATGTTGGGCGTATGAAATTTGAAGGCCAGAAAAGAGTTAAAGACCTGCTTGATGGCTTATCACAAGCGAAAGGTAACTGGAAACTCCATGTATTGGGTGATGGCTCCGATT > W3110S.gb/3836796‑3836937 | cccGAGGAAGGTGAAACGGCAACGTTTA‑TTATGTTGGGCGTATGAAATTTGAAGGCCAGAAAAGAGTTaaa < 1:613770/71‑1 (MQ=255) cGAGGAAGGTGAAACGGCAACGTTTATTTATGTTGGGCGTATGAAATTTGAAGGCCAGAAAAGAGTTAAAg < 1:1975910/71‑1 (MQ=255) gAGGAAGGTGAAACGGCAACGTTTATTTATGTTGGGCGTATGAAATTTGAAGGCCAGAAAAGAGTTAAAGa > 1:3980878/1‑71 (MQ=255) aGGTGAAACGGCAACGTTTATTTATGTTGGGCGTATGAAATTTGAAGGCCAGAAAAGAGTTAAAGACCTGc > 1:231947/1‑71 (MQ=255) aaCGGCAACGTTTATTTATGTTGGGCGTATGAAATTTGAAGGCCAGAAAAGAGTTAAAGACCTGCTTGATg < 1:3613925/71‑1 (MQ=255) aaCGGCAACGTTTATTTATGTTGGGCGTATGAAATTTGAAGGCCAGAAAAGAGTTAAAGACCTGCTTGATg > 1:1678600/1‑71 (MQ=255) aCGGCAACGTTTATTTATGTTGGGCGTATGAAATTTGAAGGCCAGAAAAGAGTTAAAGACCTGCTTGATgg < 1:2307670/71‑1 (MQ=255) gTTTATTTATGTTGGGCGTATGAAATTTGAAGGCCAGAAAAGAGTTAAAGACCTGCTTGATGGCTTATcac < 1:4058308/71‑1 (MQ=255) tttatttaTGTTGGGCGTATGAAATTTGAAGGCCAGAAAAGAGTTaaa < 1:336426/48‑1 (MQ=255) tttatttaTGTTGGGCGTATGAAATTTGAAGGCCAGAAAAGAGTTAAAGACCTGCTTGATGGCTTATcaca < 1:2928742/71‑1 (MQ=255) tttatttaTGTTGGGCGTATGAAATTTGAAGGCCAGAAAAGAGTTAAAGACCTGCTTGATGGCTTATcac > 1:652376/1‑70 (MQ=255) tatttatGTTGGGCGTATGAAATTTGAAGGCCAGAAAAGAGTTAAAGACCTGCGTGATGGCTTa > 1:3055159/1‑64 (MQ=255) tttatGTTGGGCGTATGAAATTTGAAGGCCAGAAAAGAGTTAAAGACCTGc > 1:3194250/1‑51 (MQ=255) atGTTGGGCGTATGAAATTTGAAGGCCAGAAAAGAGTTAAAGACCTGCTTGATGGCTTATCACAAGCGaa < 1:3554009/70‑1 (MQ=255) ttGGGCGTATGAAATTTGAAGGACAGAAAAGAGTTAAAGACCTGCTTGATGGCTTATCACaa > 1:3864818/1‑62 (MQ=255) tGGGAGTATGAAATTTGAAGGCCAGAAAAGAGTTAAAGACCTGCTTGATGGCTTATCACAAGCGAAAGGt < 1:510003/70‑1 (MQ=255) ggCGTATGAAATTTGAAGGCCAGAAAAGAGTTAAAGACCTGCTTGAt > 1:368970/1‑47 (MQ=255) ggCGTATGAAATTTGAAGGCCAGAAAAGAGTTAAAGACCTGCTTGATGGCTTATCACAAGCGAAAGGTaa < 1:732428/70‑1 (MQ=255) ggCGTATGAAATTTGAAGGCCAGAAAAGAGTTAAAGACCTGCTTGATGGCTTATCACAAGCGAAAGGTaa < 1:1502813/70‑1 (MQ=255) ggCGTATGAAATTTGAAGGCCAGAAAAGAGTTAAAGACCTGCTTGATGGCTTATCACAAGCGAAAGGTAAc < 1:2123743/71‑1 (MQ=255) tATGAAATTTGAAGGCCAGAAAAGAGTTAAGGACCTGCtt < 1:1398618/40‑1 (MQ=255) tATGAAATTTGAAGGCCAGAAAAGAGTTAAAGACCTGCTTGATGGCTTATCACAAGCGAAAGGTAACTGGa < 1:4017822/71‑1 (MQ=255) tATGAAATTTGAAGGCCAGAAAAGAGTTAAAGACCTGCTTGATGGCTTATCACAAGCGAAAGGTAACTGGa < 1:1306894/71‑1 (MQ=255) tGAAATTTGAAGGCCAGAAAAGAGTTAAGGACCTGCTTGa > 1:3103991/1‑40 (MQ=255) tGAAATTTGAAGGCCAGAAAAGAGTTAAAGACCTGCTTGATGGCTTATCACAAGCGAAAGGt < 1:110717/62‑1 (MQ=255) gAAATTTGAAGGCCAGAAAAGAGTTAAAGACCTGCTTGATGGCTTATCACAAGCGAAAGGTAACTGGAAAc > 1:3215086/1‑71 (MQ=255) aaaTTTGAAGGCCAGAAAAGAGTTAAAGACCTGCTTGATGGCTTATca > 1:123469/1‑48 (MQ=255) aaaTTTGAAGGCCAGAAAAGAGTTAAAGACCTGCTTGATGGCTTATCACAAGCGAAAGGTAACTGGAAACt > 1:395324/1‑71 (MQ=255) aaaTTTGAAGGCCAGAAAAGAGTTAAAGACC‑‑‑‑TGATGGCTTATCACAAGCGAAAGGTAACTGGAAACTCCa < 1:3076573/70‑1 (MQ=255) aTTTGAAGGCCAGAAAAGAGTTAAAGACCTGCTTGATGGCTTATCACAAGCGAAAGGTAACTGGAAACTcc < 1:1766680/71‑1 (MQ=255) aTTTGAAGGCCAGAAAAGAGTTAAAGACCTGCTTGATGGCTTATCACAAGCGAAAGGTAACTGGAAACTc < 1:3910943/70‑1 (MQ=255) tttGAAGGCCAGAAAAGAGTTAAACACCTGCTTGATGGCTTATCACaa < 1:102550/48‑1 (MQ=255) tGAAGGCCAGAAAAGAGTTAAAGACCTGCTTGATGGc > 1:1767546/1‑37 (MQ=255) gAAGGCCAGAAAAGAGTTAAAGACCTGCTTGATGGCTTATCACAAGCGAAAGGTAACTGGAAACTCCATg < 1:1871485/70‑1 (MQ=255) aaGGCCAGAAAAGAGTTAAAGACCTGCTTGATGGCTTATCACAAGCGAAAGGTAACTGGAAACTCCATGt < 1:263929/70‑1 (MQ=255) aaGGCCAGAAAAGAGTTAAAGACCTGCTTGATGGCTTATCACAAGCGAAAGGTAACTGGAAACTCCATGt < 1:2414610/70‑1 (MQ=255) ccAGAAAAGAGTTAAAGACCTGCTTGATGGCTTATCACAAGCGAAAGGTAACTGGa < 1:2439766/56‑1 (MQ=255) ccAGAAAAGAGTTAAAGACCTGCTTGATGGCTTATCACAAGCGAAAGGTAACTGGAAACTCCATGTATTgg < 1:1410940/71‑1 (MQ=255) cAGAAAAGAGTTAAAGACCTGCTTGATGGCTTATCACAAGCGAAAGGTAACTGGAAACTCCATGTATTgg > 1:1917064/1‑70 (MQ=255) gAAAAGAGTTAAGGACCTGCTTGATGGCTTATCACAAGCGAAAGGTAACTGGAAACTCCATGTATTGGGt > 1:2083359/1‑70 (MQ=255) gAAAAGAGTTAAAGACCTGCTTGATGGCTTATCACAAGCGAAAGGTAACTGGAAACTCCATGTATTGGGt > 1:2806998/1‑70 (MQ=255) aaaaGAGTTAAAGACCTGCTTGATGGCTTATCACAAGCGAAAg < 1:3535024/43‑1 (MQ=255) aaaGAGTTAAAGACCTGCTTGATGGCTTATCACAAGCGAAAGGTAACTGGAAACTCCATGTATTGGGTGAt > 1:2582447/1‑71 (MQ=255) gagTTAAAGACCTGCTTGATGGCTTATCACAAGCGAAAGGTAACTGGAAACTCCATGTATTGGGTGATGGc < 1:3112805/71‑1 (MQ=255) ttAAGGACCTGCTTGATGGCTTATCACAAGCGAAAGGTAACTGGAAACTCCATGTATTGGGt < 1:1016591/62‑1 (MQ=255) ttAAAGACCTGCTTGATGGCTTATCACAAGCGAAAGGTAACTGGAAACTCCATGTAt > 1:1808994/1‑57 (MQ=255) ttAAAGACCTGCTTGATGGCTTATCACAAGCGAAAGGTAACTGGAAACTCCATGTATTGGGTGATGGCTcc < 1:3587718/71‑1 (MQ=255) tAAAGACCTGCTTGATGGCTTATCACAAGCGAAAGGTAACTGGaaa > 1:1529956/1‑46 (MQ=255) aaaGACCTGCTTGATGGCTTATCACAAGCGAAAGGTAACTGGAAACTcc > 1:3054874/1‑49 (MQ=255) aaaGACCTGCTTGATGGCTTATCACAAGCGAAAGGTAACTGGAAACTCCa > 1:377073/1‑50 (MQ=255) aaaGACCTGCTTGATGGCTTATCACAAGCGAAAGGTAACTGGAAACTCCa > 1:2118309/1‑50 (MQ=255) aaaGACCTGCTTGATGGCTTATCACAAGCGAAAGGTAACTGGAAACTCCATGTATTGGGTGATGGCTCCg < 1:19823/70‑1 (MQ=255) aaaGACCTGCTTGATGGCTTATCACAAGCGAAAGGTAACTGGAAACTCCATGTATTGGGTGATGGCTCCGa > 1:1592486/1‑71 (MQ=255) aaGACCTGTTTGATGGCTTATCACAAGCGAAAGGTAACTGGAAACTCCATGTATTGGGTGATGGCTCCGa < 1:621229/70‑1 (MQ=255) aGACCTGCTTGATGGCTTATCACAAGCGAAAGGTAAc > 1:3982180/1‑37 (MQ=255) aGACCTGCTTGATGGCTTATCACAAGCGAAAGGTAAc > 1:2772383/1‑37 (MQ=255) aGACCTGCTTGATGGCTTATCACAAGCGAAAGGTAACTGGAAACTCCATGTATTg < 1:3774677/55‑1 (MQ=255) aGACCTGCTTGATGGCTTATCACAAGCGAAAGGTAACTGGAAACTCCATGTATTGGGTGATGGCTCCGAtt < 1:3775660/71‑1 (MQ=255) | CCCGAGGAAGGTGAAACGGCAACGTTTATTTATGTTGGGCGTATGAAATTTGAAGGCCAGAAAAGAGTTAAAGACCTGCTTGATGGCTTATCACAAGCGAAAGGTAACTGGAAACTCCATGTATTGGGTGATGGCTCCGATT > W3110S.gb/3836796‑3836937 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |