| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,915,028 | C→T | 6.7% | intergenic (+24/‑71) | ysaB → / → glyQ | hypothetical protein/glycine tRNA synthetase, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,915,028 | 0 | C | T | 6.7% | 287.7 / 8.1 | 90 | intergenic (+24/‑71) | ysaB/glyQ | hypothetical protein/glycine tRNA synthetase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base C (44/40); new base T (3/3); total (47/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01 | |||||||||||
TTCTTTTGACGCGGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCACACGTATCCAGCACGAAATAAT > W3110S.gb/3914959‑3915098 | ttCTTTTGACGCGGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACt < 1:2222393/71‑1 (MQ=255)ttCTTTTGACGCGGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACt < 1:312897/71‑1 (MQ=255) cTTTTGACGCGGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTg < 1:2222160/70‑1 (MQ=255) cTTTTGACGCGGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTg > 1:1254243/1‑70 (MQ=255) ttGACGCGGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTATTGtat < 1:4179687/70‑1 (MQ=255) ttGACGCGGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGtac < 1:1344240/70‑2 (MQ=255) tGACGCGGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGtata < 1:471750/70‑1 (MQ=255) gACGCGGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTatat < 1:3470372/70‑1 (MQ=255) gACGCGGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTatat < 1:153361/70‑1 (MQ=255) cgGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTg < 1:673524/61‑1 (MQ=255) cgGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTtcc < 1:2596600/71‑1 (MQ=255) ggATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTtcca > 1:1537624/1‑71 (MQ=255) tGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTtccatc > 1:2898750/1‑70 (MQ=255) ccATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTtc < 1:2931001/63‑1 (MQ=255) ccATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGc < 1:581006/71‑1 (MQ=255) ccATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGc < 1:742026/71‑1 (MQ=255) ccATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGc < 1:952634/71‑1 (MQ=255) ccATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTACATCTTCCATCCAGc < 1:3542176/71‑1 (MQ=255) cATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCg < 1:1810019/71‑1 (MQ=255) aTTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTtc < 1:2007493/61‑1 (MQ=255) aTTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCgg > 1:59472/1‑71 (MQ=255) aTTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCgg > 1:337061/1‑71 (MQ=255) ttttttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAg < 1:3148622/67‑1 (MQ=255) ttttttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCggg > 1:3616327/1‑71 (MQ=255) ttttttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTACATc < 1:2870367/57‑1 (MQ=255) ttttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTATTGTATATCTTCCATCCAGCggg > 1:4220556/1‑69 (MQ=255) ttttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTATTGTATATCTTCCATCCAGCGGGt < 1:1167584/70‑1 (MQ=255) ttttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGta > 1:661060/1‑51 (MQ=255) ttttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGt > 1:2744915/1‑50 (MQ=255) ttttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGta > 1:184412/1‑71 (MQ=255) ttttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGta > 1:1082023/1‑71 (MQ=255) ttttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGta > 1:2901767/1‑71 (MQ=255) ttttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTACATCTTCCATCCAGCGGGta > 1:1617887/1‑71 (MQ=255) tttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGta > 1:1581747/1‑70 (MQ=255) tttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGta < 1:1866086/70‑1 (MQ=255) tttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGta < 1:833750/70‑1 (MQ=255) ttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGtata > 1:2538318/1‑71 (MQ=255) gCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATAc < 1:1121336/70‑1 (MQ=255) cATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTg < 1:228956/71‑1 (MQ=255) cATCTATCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTg < 1:2672976/71‑1 (MQ=255) cTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTtc < 1:2948998/50‑1 (MQ=255) cTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATc > 1:1033574/1‑71 (MQ=255) tttCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGtata > 1:2306290/1‑64 (MQ=255) tttCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATcc > 1:4173227/1‑71 (MQ=255) tttCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATcc > 1:55925/1‑71 (MQ=255) ttCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATccc > 1:2511640/1‑71 (MQ=255) ttCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATccc > 1:2916517/1‑71 (MQ=255) ttCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATccc > 1:1772628/1‑71 (MQ=255) tCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGta > 1:3497502/1‑60 (MQ=255) tCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTACATCTTCCATCCAGCGGGTATACTGAt < 1:1787207/67‑1 (MQ=255) cATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACt > 1:2418859/1‑35 (MQ=255) cATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTg > 1:3117821/1‑63 (MQ=255) cATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCccttc < 1:3972280/71‑1 (MQ=255) cATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCccttc > 1:679971/1‑71 (MQ=255) aTGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTg < 1:2297071/35‑1 (MQ=255) aTGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTg < 1:589530/35‑1 (MQ=255) aTGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGc > 1:196744/1‑52 (MQ=255) aTGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGc > 1:1583211/1‑52 (MQ=255) aTGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCccttcc < 1:3442423/71‑1 (MQ=255) gCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGta > 1:3028948/1‑35 (MQ=255) cGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCccttcctt < 1:914224/70‑1 (MQ=255) cGTTAAGCCCTGCTTTTTCCCGCTTCGTACTGTATATCTtcg > 1:3515465/1‑41 (MQ=39) gTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCtt > 1:708499/1‑39 (MQ=255) gTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTACATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTa < 1:2232836/71‑1 (MQ=255) aGCCCTGCTTTTTCCCGTTTCGTATTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATc < 1:1787174/71‑1 (MQ=255) aGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATc < 1:2797543/71‑1 (MQ=255) aGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATc < 1:1377686/71‑1 (MQ=255) gCCCTGCTTTTTCCCGTTTCGTATTGTATATCTtccat > 1:2991712/1‑38 (MQ=255) gCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGta > 1:2135617/1‑48 (MQ=255) cccTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGc < 1:1337230/42‑1 (MQ=255) cTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCcaca > 1:1019345/1‑71 (MQ=255) tGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGc < 1:3330589/39‑1 (MQ=255) tGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAAt < 1:4048059/65‑1 (MQ=255) gCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGtata > 1:293858/1‑45 (MQ=255) gCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGtata > 1:2659240/1‑45 (MQ=255) cTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCggg > 1:1972097/1‑40 (MQ=255) cTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCACACg > 1:4056110/1‑70 (MQ=255) tttttCCCGTTTCGTATTGTATATCTTCCATCCAGCGGGTATACTGAt > 1:745547/1‑48 (MQ=255) tttttCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTaaa > 1:3248453/1‑61 (MQ=255) tttttCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAAt < 1:1361834/62‑1 (MQ=255) tttCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGtata > 1:2331660/1‑41 (MQ=255) ttCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATcc < 1:4252796/47‑1 (MQ=255) tCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTa > 1:218499/1‑55 (MQ=255) cccGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCACACGTATCCa < 1:1306328/70‑1 (MQ=255) gTTTCGTACTGTATATCTTCCATCCAGCGGGtata > 1:4067981/1‑35 (MQ=255) gTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCACACGTATCCAGCAc > 1:2972373/1‑71 (MQ=255) tttCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCACACGTATCCAg < 1:1092727/67‑1 (MQ=255) ttCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCcct > 1:594780/1‑42 (MQ=255) tCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCcct > 1:1097892/1‑41 (MQ=255) tCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCACACGTATCCAGCACGa > 1:4116856/1‑70 (MQ=255) cTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCACACGTATCCAGCACGAaataat < 1:3986839/71‑1 (MQ=255) | TTCTTTTGACGCGGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCACACGTATCCAGCACGAAATAAT > W3110S.gb/3914959‑3915098 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |