| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,915,241 | A→G | 15.0% | E48G (GAG→GGG) | glyQ → | glycine tRNA synthetase, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,915,241 | 0 | A | G | 15.0% | 146.4 / 19.4 | 60 | E48G (GAG→GGG) | glyQ | glycine tRNA synthetase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base A (22/29); new base G (5/4); total (27/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.29e-01 | |||||||||||
CCATTGTTCAACCATTGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGCCCGACCGATGGTCGCTAC > W3110S.gb/3915175‑3915308 | ccATTGTTCCACCATTGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTg > 1:2915615/1‑71 (MQ=255) aTTGTTCAACCATTGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTggg < 1:3905994/71‑1 (MQ=255) cAACCATTGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGCCCTGTCTGCGCGAGCTGGGGCCa > 1:2434627/1‑69 (MQ=255) cAACCATTGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAg > 1:3862122/1‑70 (MQ=255) aTTGGACATGGAAGTCGGCGCTGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGa < 1:1007297/71‑1 (MQ=255) aTTGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGa < 1:1053096/71‑1 (MQ=255) tGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATg > 1:640005/1‑71 (MQ=255) ggACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATgg > 1:2645522/1‑71 (MQ=255) gACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGCCAGAACCGATggc < 1:2313306/71‑1 (MQ=255) aCATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTg < 1:3433452/53‑1 (MQ=255) aCATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATggcg < 1:2083083/71‑1 (MQ=255) aCATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATggc < 1:436350/70‑1 (MQ=255) aTGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTg < 1:4224124/51‑1 (MQ=255) tGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCt < 1:2799812/49‑1 (MQ=255) ggAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGCCAGAACCGATGGCGgctg > 1:2229075/1‑71 (MQ=255) aaGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGCCAGAACCGATGGCGgctgct > 1:3964239/1‑71 (MQ=255) aaGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGATCTGGGGCCAGAACCGATGGCGgctgct < 1:1836558/71‑1 (MQ=255) aGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATggcg < 1:4192648/64‑1 (MQ=255) tCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTa < 1:832959/70‑1 (MQ=255) tCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTAt < 1:2826999/71‑1 (MQ=255) ggCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAAcc < 1:1350061/53‑1 (MQ=255) ggCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATg < 1:2058437/70‑1 (MQ=255) ggCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGt < 1:1000782/71‑1 (MQ=255) gcgcGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGtt < 1:2739882/71‑1 (MQ=255) cgcgGGAACCTCTCACCCAATGACCTGTCTGAGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTc < 1:3572137/71‑1 (MQ=255) cgagggAACCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTc < 1:3235307/68‑1 (MQ=255) ggAACCTCTCACCCAATGACCTGTCTGCGCGAGCTg < 1:2496556/36‑1 (MQ=255) aCCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATggcg > 1:800158/1‑51 (MQ=255) aCCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCtt < 1:924614/70‑1 (MQ=255) ctctCACCCAATGACCTGTCTGCGCGGGCTGGGGCCAGAAcc < 1:1596093/42‑1 (MQ=255) ctctCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTtctc > 1:3333055/1‑71 (MQ=255) tctcACCCAATGACCTGTCTGCGCGGGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCtcg > 1:1937859/1‑71 (MQ=255) tcACCCAATGACCTGTCTGCGCGGGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCtcgtc > 1:2451494/1‑71 (MQ=255) tcACCCAATGACCTGTCTGCGCGGGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCtcg < 1:306310/69‑1 (MQ=255) tcACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGgctgc > 1:3379636/1‑51 (MQ=255) cACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGtt < 1:4162671/57‑1 (MQ=255) ccAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATggcggc < 1:3913514/44‑1 (MQ=255) cAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGCccg < 1:834271/71‑1 (MQ=255) cAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGCccg < 1:584946/71‑1 (MQ=255) aaTGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCtcgtc > 1:652071/1‑65 (MQ=255) aTGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGtt > 1:2481222/1‑51 (MQ=255) aTGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCtt < 1:935762/58‑1 (MQ=255) aTGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGCccgac > 1:2609198/1‑71 (MQ=255) aTGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGCccgac < 1:738403/71‑1 (MQ=255) aTGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGCccgac > 1:686315/1‑71 (MQ=255) tGACCTGTCTGCGCGATCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGCccgacc < 1:798039/71‑1 (MQ=255) tGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGCccgacc > 1:716848/1‑71 (MQ=255) aCCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGCccgaccga > 1:3678861/1‑71 (MQ=255) aCCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGCccgaccga > 1:3421892/1‑71 (MQ=255) ccTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGCccgaccga < 1:3303709/70‑1 (MQ=255) gTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGCCCGACCGAt < 1:2064304/68‑1 (MQ=255) tCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGCCCGACCGATGGTc > 1:3030832/1‑71 (MQ=255) tGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTAt > 1:2691296/1‑38 (MQ=255) tGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGcc > 1:2497770/1‑46 (MQ=255) tGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGcc > 1:1667434/1‑46 (MQ=255) tGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTtctc > 1:3247575/1‑51 (MQ=255) tGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGCCCGACCGATGGTCGc > 1:2778437/1‑71 (MQ=255) cgcgAGCTGGGGCCAGAACCGATGGCGTCTGCTTATGTTCAGCCTTCTCGTCGCCCGACCGATGGTCGc > 1:1494048/1‑69 (MQ=255) gcgAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGCccgaccga > 1:1923806/1‑61 (MQ=255) gcgAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGCCCGACCGATGGTCGCTAc < 1:1432040/71‑1 (MQ=255) | CCATTGTTCAACCATTGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGCCCGACCGATGGTCGCTAC > W3110S.gb/3915175‑3915308 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |