| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,012,376 | G→A | 12.7% | A500T (GCC→ACC) | rbbA → | fused ribosome‑associated ATPases |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,012,376 | 0 | G | A | 12.7% | 155.1 / 12.4 | 55 | A500T (GCC→ACC) | rbbA | fused ribosome‑associated ATPases |
| Reads supporting (aligned to +/- strand): ref base G (21/27); new base A (3/4); total (24/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01 | |||||||||||
CATCTCACTGATGCACGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGCAACGAAGCCGAAG > W3110S.gb/4012306‑4012445 | cATCTCACTGATGCACGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAg < 1:2530825/71‑1 (MQ=255) cACTGATGCACGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAgccgc > 1:3596028/1‑70 (MQ=255) aCTGATGCACGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAgcc < 1:1971975/67‑1 (MQ=255) aCTGATGCACGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCa < 1:1713591/71‑1 (MQ=255) aTGCACGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGGACTGGTTGAGAAACGCGGAGCCGCCAGTCt < 1:1462034/71‑1 (MQ=255) gCCGGAAAAGTGCTTGCCAGAGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGaa > 1:422027/1‑69 (MQ=255) ccGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGaa > 1:3515421/1‑68 (MQ=255) ggAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAgg > 1:2321812/1‑70 (MQ=255) ggAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGc < 1:3296945/71‑1 (MQ=255) aaGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCAttt < 1:2420878/71‑1 (MQ=255) aaGTGCTTGCCAGCGGTACACCGCAGGAACTGGGTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCAttt < 1:77182/71‑1 (MQ=255) gTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTAt < 1:3573356/71‑1 (MQ=255) tGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATc < 1:2309158/71‑1 (MQ=255) cTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTg < 1:1471800/54‑1 (MQ=255) gCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGaa < 1:4063298/54‑1 (MQ=255) gCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGcc < 1:2778695/69‑1 (MQ=255) gCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCt < 1:2901557/70‑1 (MQ=255) gCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTa > 1:3500686/1‑71 (MQ=255) gCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTa > 1:2144497/1‑71 (MQ=255) gCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTa > 1:2260360/1‑71 (MQ=255) ccAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAaga > 1:1997309/1‑55 (MQ=255) aGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCAt < 1:1858668/58‑1 (MQ=255) gCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTg < 1:569528/71‑1 (MQ=255) gCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTg > 1:3880029/1‑71 (MQ=255) gCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAACCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTg < 1:1462063/71‑1 (MQ=255) cGGTACACCGCAGGAACTGGTTGAGAAACGCGGAACCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTg > 1:878674/1‑70 (MQ=255) tACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTa < 1:4004429/56‑1 (MQ=255) acacCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAgg < 1:82684/70‑1 (MQ=255) acacCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAgg > 1:3906360/1‑70 (MQ=255) cacCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCAttt < 1:91740/53‑1 (MQ=255) aGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCggca < 1:3252179/71‑1 (MQ=255) aGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCggca < 1:4107620/71‑1 (MQ=255) ggAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCggcag > 1:4146149/1‑71 (MQ=255) ggAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCggcag > 1:4141077/1‑71 (MQ=255) ggAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCggcag < 1:2940368/71‑1 (MQ=255) gAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCgg < 1:1450121/67‑1 (MQ=255) gAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCgg < 1:3169262/67‑1 (MQ=255) aCTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCggcaggg > 1:2301031/1‑70 (MQ=255) aCTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCggcaggg > 1:613314/1‑70 (MQ=255) tGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCggcaggg > 1:1510548/1‑68 (MQ=255) tGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCggcaggg > 1:2715643/1‑68 (MQ=255) gTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGc > 1:1330063/1‑52 (MQ=255) ttGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGc < 1:861158/71‑1 (MQ=255) tGAGAAACGCGGAACCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGc > 1:767856/1‑70 (MQ=255) tGAGAAACGCGGAACCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGc > 1:1013118/1‑70 (MQ=255) gagaAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGc > 1:2770061/1‑41 (MQ=255) gagaAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTAttt > 1:3507845/1‑47 (MQ=255) agaAACGCGGAACCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGGAAc < 1:2718823/71‑1 (MQ=255) agaAACGCGGAACCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGCAAc < 1:1099475/71‑1 (MQ=255) gaAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGCAAcg < 1:2108340/71‑1 (MQ=255) aaCGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGCAAcga < 1:1601151/70‑1 (MQ=255) gcgGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCt > 1:1062140/1‑36 (MQ=255) gcgGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGa > 1:2840885/1‑46 (MQ=255) cgGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGCAAcgaagccg < 1:1439531/71‑1 (MQ=255) cgGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGCAAcgaagccg < 1:1093912/71‑1 (MQ=255) ggAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGCAAcgaagccg < 1:691705/70‑1 (MQ=255) ggAACCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGCAAcgaagccga < 1:3783706/71‑1 (MQ=255) aGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGCAAcgaagccgaag > 1:1966307/1‑71 (MQ=255) | CATCTCACTGATGCACGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGCAACGAAGCCGAAG > W3110S.gb/4012306‑4012445 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |