| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,164,007 | C→A | 7.6% | T10T (ACC→ACA) | yheO → | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,164,007 | 0 | C | A | 7.6% | 211.0 / 2.3 | 65 | T10T (ACC→ACA) | yheO | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (44/16); new base A (2/3); total (46/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01 | |||||||||||
AAGTTAACGACAGGCTCCTGAAAAGGAGTGTTTTTTTTCATGTCCAGGTCGCTTTTAACCAACGAAACCAGTGAGTTGGATTTACTGGATCAACGTCCTTTCGACCAGACCGATTTTGATATTCTGAAATCCTAC > W3110S.gb/4163939‑4164073 | aaGTTAACGACAGGCTCCTGAAAAGGAGTGTTTTTTTTCATGTCCAGGTCGCTTTTAACCAACGAAACCAg > 1:1137872/1‑71 (MQ=255) tAACGACAGGCTCCTGAAAAGGAGTGTTTTTTTTCATGTCCAGGTCGCTTTTAACCAACGAAACCAGTg > 1:2068589/1‑69 (MQ=255) aaCGACAGGCTCCTGAAAAGGAGTGTTTTTTTTCATGTCCAGGTCTCTTTTAACCAACGAAACCa > 1:3987608/1‑65 (MQ=255) aaCGACAGGCTCCTGAAAAGGAGTGTTTTTTTTCATGTCCAGGTCGCTTTTAACCAACGAAAcc > 1:1364960/1‑64 (MQ=255) aaCGACAGGCTCCTGAAAAGGAGTGTTTTTTTTCATGTCCAGGTCGCTTTTAACCAACGAAACCAGTGAGt > 1:3561885/1‑71 (MQ=255) cGACAGGCTCCTGAAAAGGAGTGTTTTTTTTCATGTCCAGGTCGCTTTTAACCAACGAAACCAGTGAGt > 1:3041349/1‑69 (MQ=255) aCAGGCTCCTGAAAAGGAGTGTTTTTTTTCATGTCCAGGTCGCTTTTAACCAACGAAACCAGTGAGTTGGa > 1:176305/1‑71 (MQ=255) aCAGGCTCCTGAAAAGGAGTGTTTTTTTTCATGTCCAGGTCGCTTTTAACCAACGAAACAAGTGAGTTGGa < 1:905334/71‑1 (MQ=255) ggCTCCTGAAAAGGAGTGTTTTTTTTCATGTCCAGGTCGCTTTTAACCAACGAAACCAGTGAGTTGGAtt > 1:4245375/1‑70 (MQ=255) cTGAAAAGGAGTGTTTTTTTTCATGTCCAGGTCGCTTTTAACCAACGAAACCAGTGAGTTGGAtt < 1:2406808/65‑1 (MQ=255) cTGAAAAGGAGTGTTTTTTTTCATGTCCAGGTCGCTTTTAACCAACGAAACCAGTGAGTTGGATTTACt < 1:3554938/69‑1 (MQ=255) cTGAAAAGGAGTGTTTTTTTTCATGTCCAGGTCGCTTTTAACCAACGAAACCAGTGAGTTGGATTTACTg < 1:1067274/70‑1 (MQ=255) cTGAAAAGGAGTGTTTTTTTTCATGTCCAGGTCGCTTTTAACCAACGAAACAAGTGAGTTGGATTTACTg > 1:2825467/1‑70 (MQ=255) tGAAAAGGAGTGTTTTTTTTCATGTCCAGGTCGCTTTTAACCAACGAAACCAGTGAGTTGGATTTACTGGa > 1:3519496/1‑71 (MQ=255) tGAAAAGGAGTGTTTTTTTTCATGTCCAGGTCGCTTTTAACCAACGAAACCAGTGAGTTGGATTTACTGGa > 1:2553089/1‑71 (MQ=255) gAAAAGGAGTGTTTTTTTTCATGTCCAGGTCGCTTTTAACCAACGAAAcc > 1:2367248/1‑50 (MQ=255) gAAAAGGAGTGTTTTTTTTCATGTCCAGGTCGCTTTTAACCAACGAAACCAGTGAGTTGGATTTACTGGAt > 1:1902444/1‑71 (MQ=255) gAAAAGGAGTGTTTTTTTTCATGTCCAGGTCGCTTTTAACCAACGAAACCAGTGAGTTGGATTTACTGGAt > 1:924950/1‑71 (MQ=255) aaaaGGAGTGTTTTTTTTCATGTCCAGGTCGCTTTTAACCAACGAAACCAGTGAGTTGGATTTACTGGa < 1:2989365/69‑1 (MQ=255) aaaaGGAGTGTTTTTTTTCATGTCCAGGTCGCTTTTAACCAACGAAACCAGTGAGTTGCATTTACTGGa > 1:1722028/1‑69 (MQ=255) aaaaGGAGTGTTTTTTTTCATGTCCAGGTCGCTTTTAACCAACGAAACAAGTGAGTTGGATTTACTGGAt > 1:590173/1‑70 (MQ=255) gAGTGTTTTTTTTCATGTCCAGGTCGCTTTTAACCAACGAAAcc < 1:1173665/44‑1 (MQ=255) gtgtTTTTTTTCATGTCCAGGTCGCTTTTAACCAACGAAACCAGTGAGTTGGATTTACTGGATCAACGTcc > 1:2318889/1‑71 (MQ=255) tgtTTTTTTTCATGTCCAGGTCGCTTTTAACCAACGAAACCAGTGAGTTGGATTTACTGGATCa < 1:2355362/64‑1 (MQ=255) gtTTTTTTTCATGTCCAGGTCGCTTTTAACCAACGAAACCAGTGAGTTGGATTTAc > 1:391234/1‑56 (MQ=255) gtTTTTTTTCATGTCCAGGTCGCTTTTAACCAACGAAACCAGTGAGTTGGATTTACTGGATCAACGTCCtt > 1:896473/1‑71 (MQ=255) ttttttttCATGTCCAGGTCGCTTTTAACCAACGAAACCAGTGAGTTGGATTTACTGGATCAACGTCCttt > 1:699101/1‑71 (MQ=255) ttttttCATGTCCAGGTCGCTTTTAACCAACGAAACCAGt > 1:64818/1‑40 (MQ=255) ttttttCATGTCCAGGTCGCTTTTAACCAACGAAACCAGTGAGTTGGATTTACTGGATCAACGt < 1:789329/64‑1 (MQ=255) ttttttCATGTCCAGGTCGCTTTTAACCAACGAAACCAGTGAGTTGGATTTACTGGATCAACGTCCTTTc > 1:2895242/1‑70 (MQ=255) ttttCATGTCCAGGTCGCTTTTAACCAACGAAACCAGTGAGTTGGAttt < 1:2420990/49‑1 (MQ=255) tttCATGTCCAGGTCGCTTTTAACCAACGAAACCAGt > 1:2155756/1‑37 (MQ=255) tttCATGTCCAGGTCGCTTTTAACCAACGAAACCAGTGAGTTGGAtt > 1:1263126/1‑47 (MQ=255) ttCATGTCCAGGTCGCTTTTAACCAACGAAACCAGTGAGTTGGATTTACTGGATCAACGTCCTTTCGAc > 1:1073750/1‑69 (MQ=255) tCATGTCCAGGTCGCTTTTAACCAACGAAACCAGTGAGTTGGATTTACTGGATCAACGTCCtt > 1:2205504/1‑63 (MQ=255) aTGTCCAGGTCGCTTTTAACCAACGAAACCAGTGAGTTGGATTTACTGGATc > 1:2891627/1‑52 (MQ=255) aTGTCCAGGTCGCTTTTAACCAACGAAACCAGTGAGTTGGATTTACTGGATCAACGTcc > 1:3732281/1‑59 (MQ=255) gTCGCTTTTAACCAACGAAACCAGTGAGTTGGATTTACTGGATCa < 1:2233737/45‑1 (MQ=255) gTCGCTTTTAACCAACGAAACCAGTGAGTTGGACTTACTGGATCa < 1:611000/45‑1 (MQ=255) cGCTTTTAACCAACGAAACCAGTGAGTTGGATTTACTGGATCAACGTCCtt > 1:1124321/1‑51 (MQ=255) cGCTTTTAACCAACGAAACCAGTGAGTTGGATTTACTGGATCAACGTCCTTTCGACCAGCCCGATTTTGa > 1:2254081/1‑70 (MQ=255) cGCTTTTAACCAACGAAACCAGTGAGTTGGATTTACTGGATCAACGTCCTTTCGACCAGACCGATTTTGa > 1:4233396/1‑70 (MQ=255) gCTTTTAACCAACGAAACCAGTGAGTTGGATTTACTGGATc > 1:3635373/1‑41 (MQ=255) gCTTTTAACCAACGAAACCAGTGAGTTGGATTTACTGGATCAACGTCCTTTCGACCAGACCGATTTTGata > 1:2564890/1‑71 (MQ=255) gCTTTTAACCAACGAAACCAGTGAGTTGGATTTACTGGATCAACGTCCTTTCGACCAGACCGATTTTGata > 1:1556459/1‑71 (MQ=255) cTTTTAACCAACGAAACAAGTGAGTTGGATTTACTGGATCAACGTCCTTTCGACCAGACCGAtttt < 1:223919/66‑1 (MQ=255) ttttAACCAACGAAACCAGTGAGTTGGATTTACTg < 1:1670997/35‑1 (MQ=255) ttttAACCAACGAAACCAGTGAGTTGGATTTACTGGATCAACGTCCTTTCGACCAGACCGATTTTGa < 1:3429401/67‑1 (MQ=255) tttAACCAACGAAACCAGTGAGTTGGATTTACTGGATCAACGTCCTTTCGACCAGACCGATTTTGATATTc > 1:99843/1‑71 (MQ=255) tttAACCAACGAAACCAGTGAGTTGGATTTACTGGATCAACGTCCTTTCGACCAGACCGATTTTGATATTc > 1:3049554/1‑71 (MQ=255) ttAACCAACGAAACCAGTGAGTTGGATTTACTGGATCAACGTCCTTTCGa > 1:1183810/1‑50 (MQ=255) ttAACCAACGAAACCAGTGAGTTGGATTTACTGGATCAACGTCCTTTCGACCAg > 1:2608726/1‑54 (MQ=255) ttAACCAACGAAACCAGTGAGTTGGATTTACTGGATCAACGTCCTTTCGACCAGACCGATTTTGATAtt < 1:244165/69‑1 (MQ=255) ttAACCAACGAAACCAGTGAGTTGGATTTACTGGATCAACGTCCTTTCGACCAGACCGATTTTGATATTCt > 1:2104362/1‑71 (MQ=255) ttAACCAACGAAACAAGTGAGTTGGATTTACTGGATCAACGTCCTTTCGACCAGACCGATTTTGATAtt < 1:38190/69‑1 (MQ=255) tAACCAACGAAACCAGTGAGTTGGATTTACTGGATCAACGTCCTTTCGACCAGAc > 1:850850/1‑55 (MQ=255) ccAACGAAACCAGTGAGTTGGATTTACTGGATCAACGTCCTTTCg < 1:439619/45‑1 (MQ=255) ccAACGAAACCAGTGAGTTGGATTTACTGGATCAACGTCCTTTCGAcc > 1:2022999/1‑48 (MQ=255) ccAACGAAACCAGTGAGTTGGATTTACTGGATCAACGTCCTTTCGAc > 1:2064268/1‑47 (MQ=255) cAACGAAACCAGTGAGTTGGATTTACTGGATCAACGTcc < 1:3979619/39‑1 (MQ=255) cAACGAAACCAGTGAGTTGGATTTACTGGATCAACGTCCTTTCGACCAGACCGATTTTGATATTCTGaaa > 1:182760/1‑70 (MQ=255) aCGAAACCAGTGAGTTGGATTTACTGGATCAACGTCCTTTCGACCAGACCGATTTTGATATTCTGAAATcc > 1:478422/1‑71 (MQ=255) aCGAAACCAGTGAGTTGGATTTACTGGATCAACGTCCTTTCGACCAGACCGATTTTGATATTCTGAAATcc > 1:3590484/1‑71 (MQ=255) aCGAAACCAGTGAGTTGGATTTACTGGATCAACGTCCTTTCGACCAGACCGATTTTGATATTCTGAAATcc > 1:798533/1‑71 (MQ=255) cGAAACCAGTGAGTTGGATTTACTGGATCAACGTCCTTTCGACCAGACCGAtt < 1:3748835/53‑1 (MQ=255) cGAAACCAGTGAGTTGGATTTACTGGATCAACGTCCTTTCGACCAGACCGATTTTGATATTCTGAAATcc > 1:3169499/1‑70 (MQ=255) aaaCCAGTGAGTTGGATTTACTGGATCAACGTCCTTTCGACCAGACCGATTTTGATATTCTGAAATCCTAc > 1:1623246/1‑71 (MQ=255) aCCAGTGAGTTGGATTTACTGGATCAACGTCCTTTCGACCAGACCGATTTTGata < 1:1144172/55‑1 (MQ=255) | AAGTTAACGACAGGCTCCTGAAAAGGAGTGTTTTTTTTCATGTCCAGGTCGCTTTTAACCAACGAAACCAGTGAGTTGGATTTACTGGATCAACGTCCTTTCGACCAGACCGATTTTGATATTCTGAAATCCTAC > W3110S.gb/4163939‑4164073 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |