| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,218,735 | G→T | 8.5% | W288C (TGG→TGT) | metA → | homoserine transsuccinylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,218,735 | 0 | G | T | 8.5% | 210.2 / 4.3 | 71 | W288C (TGG→TGT) | metA | homoserine transsuccinylase |
| Reads supporting (aligned to +/- strand): ref base G (38/25); new base T (4/2); total (42/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01 | |||||||||||
CGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATC > W3110S.gb/4218665‑4218803 | cGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTgg > 1:2119309/1‑71 (MQ=255)cGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTgg > 1:4086917/1‑71 (MQ=255) gCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGc < 1:322322/71‑1 (MQ=255) cacaATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCt > 1:1286394/1‑71 (MQ=255) cacaATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCt > 1:2896532/1‑71 (MQ=255) acaATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGCCTc < 1:3025247/71‑1 (MQ=255) caATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTc > 1:2983589/1‑70 (MQ=255) aTGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAAc > 1:2759493/1‑71 (MQ=255) aTGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAAc > 1:1754823/1‑71 (MQ=255) aTGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAAc < 1:1776311/71‑1 (MQ=255) aTGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAAc > 1:3983228/1‑71 (MQ=255) gATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTa > 1:3741704/1‑71 (MQ=255) aTCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGTCTCAACTa < 1:3414343/70‑1 (MQ=255) aTCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGTCTCAACTAt < 1:1354855/71‑1 (MQ=255) aTCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTAt < 1:1518906/71‑1 (MQ=255) tCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTa < 1:1199251/69‑1 (MQ=255) cGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTAc > 1:2478453/1‑71 (MQ=255) aaaaTACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTc > 1:4261271/1‑71 (MQ=255) aaaTACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTAtt < 1:473140/65‑1 (MQ=255) aaaTACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTc > 1:2600070/1‑70 (MQ=255) aTACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGTCTCAACTATTACGTCTAc > 1:346261/1‑71 (MQ=255) aTACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTAc > 1:3620831/1‑71 (MQ=255) cacCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTAc > 1:499119/1‑68 (MQ=255) cgcgAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCt > 1:2458733/1‑49 (MQ=255) cgcgAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACt > 1:1932804/1‑54 (MQ=255) gcgagcgaGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCa > 1:4061157/1‑71 (MQ=255) cgagcgagCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTAc > 1:2740247/1‑57 (MQ=255) cgagcgagCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCAc > 1:583005/1‑71 (MQ=255) cgagcgagCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCAc > 1:3733751/1‑71 (MQ=255) agcgagcTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTAt < 1:3150566/52‑1 (MQ=255) gcgagcTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGcc < 1:2941841/71‑1 (MQ=255) cgagcTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCa > 1:4113345/1‑61 (MQ=255) cgagcTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCa < 1:1618362/71‑1 (MQ=255) gagcTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCa > 1:2645456/1‑70 (MQ=255) gcTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATAc < 1:2990404/71‑1 (MQ=255) gcTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATAc < 1:1048512/71‑1 (MQ=255) cTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATAc < 1:3208781/70‑1 (MQ=255) cTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACg < 1:617708/71‑1 (MQ=255) tGGCGTAGTCACGGTAATTTACTGTTTACCAACTGTCTCAACTATTACGTCTACCAGATCACGCCATACGa > 1:1548395/1‑71 (MQ=255) gCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGAt > 1:1930864/1‑70 (MQ=255) gCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATc > 1:51939/1‑71 (MQ=255) gCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATc < 1:3409921/71‑1 (MQ=255) tAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAg < 1:3514290/52‑1 (MQ=255) tAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCa > 1:647062/1‑61 (MQ=255) cACGGTAATTTACTGTTTACCAACTGTCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGca > 1:212637/1‑71 (MQ=255) cACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTAcc > 1:984707/1‑46 (MQ=255) cACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGca > 1:3451347/1‑71 (MQ=255) aCGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTc > 1:757567/1‑41 (MQ=255) aCGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCa > 1:3837808/1‑46 (MQ=255) ggTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACAt < 1:2601297/71‑1 (MQ=255) ggTAATTTACTGTTTACCAACTGGCTCAACTATTACCTCTACCAGATCACGCCATACGATCTACGGCACAt < 1:3059187/71‑1 (MQ=255) gTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGAt < 1:715544/46‑1 (MQ=255) tAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGa < 1:968815/71‑1 (MQ=255) tAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGa < 1:156223/71‑1 (MQ=255) tAATTTACTGTTTACCAACTGCCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGa < 1:3626097/71‑1 (MQ=255) aaTTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGaa > 1:2249195/1‑71 (MQ=255) aTTTACTGTTTACCTACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGaa > 1:1928773/1‑70 (MQ=255) aTTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAAt > 1:1170580/1‑71 (MQ=255) ttACTGTTTACCAACTGGCTCAACTATTACGTCTACCAg < 1:2913705/39‑1 (MQ=255) tACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATcc > 1:3844216/1‑70 (MQ=255) tACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATcc < 1:3984597/70‑1 (MQ=255) aCTGTTTACCAACTGTCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCaa > 1:2492745/1‑71 (MQ=255) tttACCAACTGGCTCAACTATTACGTCTACCAGAt > 1:244533/1‑35 (MQ=255) tttACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGaa > 1:3337046/1‑62 (MQ=255) tttACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACg < 1:2013130/69‑1 (MQ=255) tttACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACg < 1:344473/69‑1 (MQ=255) tACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTg > 1:1000152/1‑70 (MQ=255) tACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTg < 1:646761/70‑1 (MQ=255) tACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTg < 1:1719704/70‑1 (MQ=255) tACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTg > 1:3562671/1‑70 (MQ=255) ccAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATc > 1:1447841/1‑60 (MQ=255) aCTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTaa > 1:2177339/1‑71 (MQ=255) aCTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTaa > 1:801489/1‑71 (MQ=255) ggCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGa > 1:2489201/1‑51 (MQ=255) gCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAAtc < 1:1566849/69‑1 (MQ=255) | CGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATC > W3110S.gb/4218665‑4218803 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |