| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,477,260 | G→A | 12.1% | intergenic (‑45/+236) | pyrL ← / ← yjgH | pyrBI operon leader peptide/predicted mRNA endoribonuclease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,477,260 | 0 | G | A | 12.1% | 168.3 / 12.2 | 58 | intergenic (‑45/+236) | pyrL/yjgH | pyrBI operon leader peptide/predicted mRNA endoribonuclease |
| Reads supporting (aligned to +/- strand): ref base G (25/26); new base A (4/3); total (29/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
ACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCAAGGTA > W3110S.gb/4477190‑4477326 | aCAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAg < 1:2987456/71‑1 (MQ=255)aCAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAg < 1:3260861/71‑1 (MQ=255)aCAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAg < 1:1713715/71‑1 (MQ=255) cAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAgc > 1:3859729/1‑71 (MQ=255) cAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAg < 1:598693/70‑1 (MQ=255) aaaaTGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAgcg > 1:4179631/1‑71 (MQ=255) aaaaTGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAgcg > 1:1733894/1‑71 (MQ=255) aTGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAgcggc > 1:107669/1‑70 (MQ=255) cGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAg > 1:967119/1‑71 (MQ=255) gAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAg > 1:2408684/1‑70 (MQ=255) gAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAg > 1:3382938/1‑70 (MQ=255) cacacTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCAtt > 1:1063209/1‑71 (MQ=255) cacacTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCAtt > 1:3484954/1‑71 (MQ=255) cacacTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCAtt > 1:589269/1‑71 (MQ=255) acacTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCAttt < 1:3490389/71‑1 (MQ=255) acacTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCAttt < 1:309578/71‑1 (MQ=255) cacTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTg < 1:4148331/71‑1 (MQ=255) tGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGc < 1:2531557/63‑1 (MQ=255) cATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCa < 1:297773/55‑1 (MQ=255) cATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCa < 1:2762312/55‑1 (MQ=255) tACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATAtt > 1:4268373/1‑71 (MQ=255) cATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCAtt > 1:1133483/1‑57 (MQ=255) cATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATAtttt > 1:67857/1‑71 (MQ=255) cATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGCATTTGATGCAATAtttt > 1:1066989/1‑71 (MQ=255) aTCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCAttt < 1:947274/57‑1 (MQ=255) aTCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCAttt < 1:779490/57‑1 (MQ=255) aTCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCAttt < 1:680971/57‑1 (MQ=255) aTCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTg > 1:2784959/1‑71 (MQ=255) tcctccCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGt < 1:3066685/71‑1 (MQ=255) ctccCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCa < 1:2325773/71‑1 (MQ=255) gCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCa > 1:3543030/1‑70 (MQ=255) aaaTTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCAttt > 1:1281316/1‑46 (MQ=255) aaaTTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAAc > 1:2683637/1‑71 (MQ=255) aaTTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACg > 1:143509/1‑71 (MQ=255) aTTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCAtt > 1:796171/1‑43 (MQ=255) aTTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTg < 1:2618290/45‑1 (MQ=255) aTTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACgg < 1:2050006/71‑1 (MQ=255) aTTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACgg > 1:3917846/1‑71 (MQ=255) aTTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACgg > 1:389089/1‑71 (MQ=255) aTTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACgg > 1:471436/1‑71 (MQ=255) aTTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACgg > 1:2419202/1‑71 (MQ=255) ttGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGc < 1:3392463/39‑1 (MQ=255) ttGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAata < 1:4248189/52‑1 (MQ=255) ttGTCCGGCATTATACTCATCGTAAGAAGCGGCGCAAGCATTTGa > 1:2738704/1‑45 (MQ=255) tCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGttt < 1:3584028/70‑1 (MQ=255) ccGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGa > 1:2225478/1‑41 (MQ=255) cGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCa < 1:3498248/55‑1 (MQ=255) cGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTAtt > 1:3059416/1‑71 (MQ=255) cATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTg > 1:1643510/1‑70 (MQ=255) ttATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATTTTTCGt < 1:1832591/48‑1 (MQ=255) ttATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGt < 1:1867300/48‑1 (MQ=255) ttATACTCATCGTCAGAAACTGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTa < 1:2280541/64‑1 (MQ=255) ttATACTCATCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTa < 1:1429580/64‑1 (MQ=255) tataCTCATCGTCAGAAGCGGCGCAAGCATTTGATGc < 1:4249947/37‑1 (MQ=255) tataCTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGt < 1:1080976/47‑1 (MQ=255) ataCTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAgcgc < 1:617724/52‑1 (MQ=255) taCTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTaa < 1:1514488/71‑1 (MQ=255) tCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAg > 1:2024250/1‑71 (MQ=255) tCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAAc < 1:3884665/49‑1 (MQ=255) tCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTc < 1:2479052/70‑1 (MQ=255) tCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCa < 1:3186584/71‑1 (MQ=255) tCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCa < 1:4011402/46‑1 (MQ=255) gTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAgc > 1:2250016/1‑41 (MQ=255) gTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGt > 1:4133516/1‑67 (MQ=255) gTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGt > 1:3551531/1‑67 (MQ=255) aGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCAAGGta > 1:1300226/1‑71 (MQ=255) | ACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCAAGGTA > W3110S.gb/4477190‑4477326 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |