| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,580,803 | C→T | 16.7% | pseudogene (1987/2721 nt) | yjiV → | ECK4334:JW5954:b4486; conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,580,803 | 0 | C | T | 16.7% | 105.4 / 13.0 | 42 | pseudogene (1987/2721 nt) | yjiV | ECK4334:JW5954:b4486; conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (13/22); new base T (3/4); total (16/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAAATGGGGGTTGATACCGATCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGCCGGAGTGGCACCTGAATGACGAACTGGTGGTGCGTAGTCTCGGTTATAAAACGGTAGAACTGAACGAGTT > W3110S.gb/4580734‑4580873 | gAAATGGGGGTTGATACCGATCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGc < 1:4215406/70‑1 (MQ=255)gAAATGGGGGTTGATACCGATCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGc < 1:3116004/70‑1 (MQ=255) aaTGGGGGTTGATACCGATCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGCCgg < 1:334431/71‑1 (MQ=255) gggTTGATACCGATCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGCCGGAGTgg < 1:747028/71‑1 (MQ=255) gATACCGATCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGCCGGAg > 1:529039/1‑63 (MQ=255) aTACCGATCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGCCGGAGTGGCACCt < 1:861847/70‑1 (MQ=255) ccGATCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGCCGGAGTGGCACCTGAAt > 1:3839346/1‑71 (MQ=255) ccGATCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGCCGGAGTGGCACCTGAAt < 1:3465237/71‑1 (MQ=255) ccGATCGCGTGATGACGCTGGCCTCTCGCAGCCAG‑AGGCAACGATTCCGGGGTCGGAGTGGCACCTGa > 1:3109149/1‑68 (MQ=255) cGATCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGTCGGAGTGGCACCTGAAt > 1:3924137/1‑70 (MQ=255) aTCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGCCGGAGTGGCACCTGAATGAc > 1:4109353/1‑71 (MQ=255) atgaCGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGTCGGAGTGGCACCTGAATGACGAACtggt < 1:3072257/71‑1 (MQ=255) atgaCGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGCCGGAGTGGCACCTGAATGACGAACtgg < 1:3455244/70‑1 (MQ=255) tgaCGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGCCGGAGTGGCACCTGAATGACg < 1:3153630/63‑1 (MQ=255) tgaCGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGCCGGAGTGGCACCTGAATGACg < 1:1075911/63‑1 (MQ=255) gaCGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGCCGGAGTGGCACCTGAATGACGAACtggtg > 1:2193213/1‑70 (MQ=255) aCGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGTCGGAGTGGCACCTGAATGACGAACTggtggt < 1:4246986/71‑1 (MQ=255) cGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGCCGGAGTGGCACCTGAATGACGa > 1:1867408/1‑61 (MQ=255) gCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGCCGGAGTGGCACCTGAATGACGAACtg < 1:2802853/64‑1 (MQ=255) cTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGCCGGAGTGGCACCTGAATGACGAACTGGTGGTGCg < 1:4205371/71‑1 (MQ=255) tGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGCCGGAGTGGCACCTGAATGACGAACTGGTGGTGCg > 1:2306059/1‑70 (MQ=255) ccTCTCGCAGCCAGCAGGCAACGATTCCGGGGCCGGAGTGGCACCTGAATGACGAACTGGTGGTGCGt < 1:1446999/68‑1 (MQ=255) tcGCAGCCAGCAGGCAACGATTCCGGGGTCGGAGTGGCACCTGAATGACGAACTGGTGGTGCGTAGTCTCg > 1:4084892/1‑71 (MQ=255) gCAGCCAGCAGGCAACGATTCCGGGGCCGGAGTGGCACCTGAATGACGAACTGGTGGTGCGTAGtctc < 1:1370697/68‑1 (MQ=255) gCAGCCAGCAGGCAACGATTCCGGGGCCGGAGTGGCACCTGAATGACGAACTGGTGGTGCGTAGtctc < 1:2219368/68‑1 (MQ=255) gCAGCCAGCAGGCAACGATTCCGGGGCCGGAGTGGCACCTGAATGACGAACTGGTGGTGCGTAGTCTCgg < 1:2507881/70‑1 (MQ=255) agcagGCAACGATTCCGGGGCCGGAGTGGCACCTGAATGACGAACTGGTGGTGCGTAGTCTCGGTtata > 1:2856725/1‑69 (MQ=255) gcagGCAACGATTCCGGGGTCGGAGTGGCACCTGAATGACGAACTGGTGGTGCg < 1:2174142/54‑1 (MQ=255) agGCAACGATTCCGGGGCCGGAGTGGCACCTGAATGACGAACTGGTGGTGCGTAGTCTCGGTTATAAAACg > 1:639497/1‑71 (MQ=255) ggCAACGATTCCGGGGCCGGAGTGGCACCTGAATGACGAACTGGTGGTGCGTAGTCTCGGTTATAAAACgg > 1:247259/1‑71 (MQ=255) gCAACGATTCCGGGGCCGGAGTGGCACCTGAATGACGAACTGGTGGTGCGTAGTCTCGGTTATaaaa < 1:3074998/67‑1 (MQ=255) aaCGATTCCGGGGCCGGAGTGGCACCTGAATGACGAACTGGTGGTGCGTAGTCTCGGTTATAAAACgg > 1:4141674/1‑68 (MQ=255) ccGATTCCGGGGCCGGAGTGGCACCTGAATGACGAACtggt < 1:4179065/40‑1 (MQ=255) aCGATTCCGGGGTCGGAGTGGCACCTGAATGACGAACTggtggt < 1:949382/44‑1 (MQ=255) cGATTCCGTGGCCGGAGTGGCACCTGAATGACGAACTGGTGGTGCGTAGTCTCGGTTATAAAACGGTAGaa > 1:3638174/1‑71 (MQ=255) cGATTCCGGGGCCGGAGTGGCACCTGAATGACGAACTGGTGGTGCGTAGTCTCGGTTATAAAACGGTAGaa > 1:3022199/1‑71 (MQ=255) aTTCCGGGGCCGGAGTGGCACCTGAATGACGAACTGGTGGTGCGTAGTCTCGGtt < 1:2826181/55‑1 (MQ=255) ccGGGGCCGGAGTGGCACCTGAATGACGAACTGGTGGTGCGTAGTCTCGGTTATAAAACGGTAGAACTGa < 1:1692163/70‑1 (MQ=255) ccGGGGCCGGAGTGGCACCTGAATGACGAACTGGTGGTGCGTAGTCTCGGTTATAAAACGGTAGAACTGa < 1:1272822/70‑1 (MQ=255) ggggCCGGAGTGGCACCTGAATGACGAACTGGTGGTGCGTAGTCTCGGTTATAAAACGGTAGAACTGAACg < 1:3975184/71‑1 (MQ=255) ggggCCGGAGTGGCACCTGAATGACGAACTGGTGGTGCGTAGTCTCGGTTATAAAACGGTAGAACTGAACg < 1:3152458/71‑1 (MQ=255) gggCCGGAGTGGCACCTGAATGACGAACTGGTGGTGCGTAGTCTCGGTTATAAAACGGTAGAACTGAACg < 1:1812370/70‑1 (MQ=255) ggCCGGAGTGGCACCTGAATGACGAACTGGTGGTGCGTAGTCTCGGTTATAAAACGGt < 1:1206509/58‑1 (MQ=255) ggCCGGAGTGGCACCTGAATGACGAACTGGTGGTGCGTAGTCTCGGTTATAAAACGGTAGAACTGAACGAg > 1:3339559/1‑71 (MQ=255) ccGGAGTGGCACCTGAATGACGAACTGGTGGTGCGTAGTCTCGGTTATAAAACGGTAGAACTGAACGAGtt < 1:3006134/71‑1 (MQ=255) | GAAATGGGGGTTGATACCGATCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGCCGGAGTGGCACCTGAATGACGAACTGGTGGTGCGTAGTCTCGGTTATAAAACGGTAGAACTGAACGAGTT > W3110S.gb/4580734‑4580873 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |