| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,606,089 | G→A | 11.1% | N37N (AAC→AAT) | dnaT ← | DNA biosynthesis protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,606,089 | 0 | G | A | 11.1% | 104.9 / 4.3 | 36 | N37N (AAC→AAT) | dnaT | DNA biosynthesis protein |
| Reads supporting (aligned to +/- strand): ref base G (16/16); new base A (2/2); total (18/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01 | |||||||||||
CAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTACCAGGGCGT > W3110S.gb/4606020‑4606159 | cAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGt > 1:400493/1‑71 (MQ=255) gCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGt > 1:1192470/1‑69 (MQ=255) cTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAg < 1:3853291/71‑1 (MQ=255) ttcttcCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCa < 1:258132/70‑1 (MQ=255) cttcCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCaaa > 1:1008997/1‑70 (MQ=255) cttcCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCaaa < 1:2405973/70‑1 (MQ=255) cttcCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCaaa < 1:1831399/70‑1 (MQ=255) ttcCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATa > 1:3573059/1‑71 (MQ=255) ccAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATAc < 1:1175/70‑1 (MQ=255) ccAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTATTAGCAAATa < 1:3500254/69‑1 (MQ=255) aGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTATTAGCAAATACgg > 1:4216027/1‑70 (MQ=255) gcCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATAAGGCAAc > 1:736823/1‑71 (MQ=255) gcagTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACcac > 1:2221297/1‑70 (MQ=255) ttCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGc > 1:1360055/1‑71 (MQ=255) ttCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGc > 1:3800848/1‑71 (MQ=255) cAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCt > 1:2041540/1‑71 (MQ=255) ccAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTATTAGc < 1:2499382/46‑1 (MQ=255) gcgtgcaggcgtgACGGCATAAAACGCCGGGGCATTGt < 1:2674885/38‑1 (MQ=255) gcgtgcaggcgtgACGGCATAAAACGCCGGGGCATTGTTAGCaa > 1:1554529/1‑44 (MQ=255) gcgtgcaggcgtgACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCtt < 1:1427137/71‑1 (MQ=255) caggcgtgACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGc < 1:546833/70‑1 (MQ=255) caggcgtgACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGc > 1:3334831/1‑70 (MQ=255) aggcgtgACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCa < 1:1786384/71‑1 (MQ=255) gcgtgACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAg > 1:179076/1‑70 (MQ=255) gtgACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAAc > 1:2263516/1‑71 (MQ=255) aCGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTc > 1:2416060/1‑53 (MQ=255) tAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGTg < 1:4132202/71‑1 (MQ=255) aaaaCGCCGGGGCATTATTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGa > 1:2753500/1‑71 (MQ=255) aaaCGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGAt > 1:1490236/1‑71 (MQ=255) aaaCGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGAt > 1:3463273/1‑71 (MQ=255) aCGCCTGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCg < 1:418877/71‑1 (MQ=255) aCGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAAGGGTTTGGTGATCg < 1:4276757/71‑1 (MQ=255) ggggCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGTTATCGTGTAc < 1:1872339/71‑1 (MQ=255) gggCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTAcc < 1:3363629/71‑1 (MQ=255) ttGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTAcc < 1:1098233/66‑1 (MQ=255) ttGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTACCAgg < 1:590115/69‑1 (MQ=255) gTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTACCAGGGCGt > 1:1208742/1‑71 (MQ=255) gTTAGCAAATACGGCAACCACACCGCC‑TCAGCTTTTGCCAGAACGGTTTGGTGATCGTCTa > 1:498581/1‑61 (MQ=255) | CAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTACCAGGGCGT > W3110S.gb/4606020‑4606159 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |