Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC W3110S.gb 3,261,134 Δ1,199 bp 100% insH[tdcD] insH, [tdcD]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ W3110S.gb 3261134–3262331 3262332 2–1199 55 [0] [0] 52 insH–[tdcD] insH,[tdcD]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb = 32611330 (0.000)30 (0.650) 22/94 NT 100% intergenic (‑59/‑69) tdcE/insH pyruvate formate‑lyase 4/2‑ketobutyrate formate‑lyase/IS5 element protein
?W3110S.gb 3262333 = 0 (0.000)coding (1183/1296 nt) tdcD propionate kinase/acetate kinase C, anaerobic

TCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/3261064‑3261133
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGA  >  W3110S.gb/3262333‑3262401
                                                                                                                                           
TCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAA                                                                       <  1:1547468/70‑1
  TACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTT                                                                    >  1:1163315/1‑71
    CCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTT                                                                   <  1:2485468/70‑1
    CCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTT                                                                   <  1:2479987/70‑1
    CCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTT                                                                    <  1:1284120/69‑1
    CCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTT                                                                    <  1:1581392/69‑1
    CCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAAC                                                                      <  1:1863500/67‑1
     CTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGC                                                                 >  1:3481803/1‑71
        CATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCT                                                               >  1:4581831/1‑70
         ATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTA                                                              <  1:3918042/70‑1
         ATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTA                                                              <  1:2668187/70‑1
            AAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAAT                                                           >  1:3926204/1‑70
             AAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGA                                                         <  1:941665/71‑1
             AAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGA                                                         <  1:2420254/71‑1
              AAATAATCTCTCTACAATACTTCAACTAAATTATTCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAA                                                        <  1:1210306/71‑1
              AAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAA                                                        >  1:4115574/1‑71
              AAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAA                                                        <  1:3734359/71‑1
              AAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGA                                                         >  1:4120980/1‑70
                 TAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGC                                                                 >  1:320678/1‑59
                 TAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTT                                                                   <  1:3827105/57‑1
                  AATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACT                                                                     <  1:1451353/54‑1
                         CTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAA                                                                       >  1:1959571/1‑45
                          TACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCA                                               <  1:3562122/68‑1
                          TACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATACA                                               <  1:4224558/68‑1
                            CAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCA                                          <  1:4482900/71‑1
                             AATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAA                                         <  1:1037101/71‑1
                               TACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAA                                                                       >  1:2037826/1‑39
                                    CAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTa                                                                   >  1:1735604/1‑37
                                    CAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTT                                  >  1:3287849/1‑71
                                    CAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGC                                                    >  1:1750091/1‑53
                                       CTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATG                                                          <  1:3253143/44‑1
                                        TAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCT                              >  1:3056975/1‑71
                                         AAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAA                                                        >  1:2538109/1‑44
                                          AATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGA                                                         <  1:266389/42‑1
                                            TTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGT                          >  1:816730/1‑71
                                            TTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGT                          >  1:2412250/1‑71
                                            TTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGT                          <  1:2453140/71‑1
                                                  AAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCG                    <  1:2727127/71‑1
                                                   AATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTC                            <  1:4009719/62‑1
                                                    ATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATGCAAAGCAATCATT                                    <  1:3749117/53‑1
                                                    ATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGG                   >  1:3749333/1‑70
                                                          CGGGCGCGTTAACTTTTCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAAC             <  1:222604/70‑1
                                                             GCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGT                          <  1:2881627/54‑1
                                                               GCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCAC        >  1:3881372/1‑70
                                                               GCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGC          <  1:2171982/68‑1
                                                                   TAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAAT    >  1:4268215/1‑70
                                                                   TAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAAT    >  1:2663131/1‑70
                                                                   TAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAAT    >  1:3597315/1‑70
                                                                   TAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAAT    >  1:3515057/1‑70
                                                                   TAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGT                          <  1:3605797/48‑1
                                                                   TAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTT                             >  1:3006195/1‑45
                                                                    AACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGA  >  1:871022/1‑71
                                                                    AACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGA  >  1:229971/1‑71
                                                                    AACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGT                      >  1:872670/1‑51
                                                                    AACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTT                                  <  1:3313238/39‑1
                                                                                                                                           
TCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/3261064‑3261133
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGA  >  W3110S.gb/3262333‑3262401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.