| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 58,662 | G→A | 24.6% | V63V (GTG→GTA) | yabP → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 58,662 | 0 | G | A | 24.6% | 108.5 / 33.9 | 57 | V63V (GTG→GTA) | yabP | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (22/21); new base A (6/8); total (28/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.60e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01 | |||||||||||
GGAGACGTTACGCCATCTGTTCTGGTCAGATAAACAGACTGAGGCTTATAAACTTCTGTTCAATTTCGTGAATAACCAGACTGGCAACATCAACGCCTCAGAATACTTTACTGGGGCTATCAACGAGAATGAGAGAGAA > W3110S.gb/58593‑58731 | ggAGACGTTACGCCATCTGTTCTGGTCAGATAAACAGACTGAGGCTTATAAACTTCTGTTCAATTTCGTg < 1:2688425/70‑1 (MQ=255) agaCGTTACGCCATCTGTTCTGGTCAGATAAACAGACTGAGGCTTATAAACTTCTGTTCAATTTCGTAAAt < 1:3576439/71‑1 (MQ=255) aCGTTACGCCATCTGTTCTGGTCAGATAAACAGACTGAGGCTTATAAACTTCTGTTCAATTTCGTGAATaa > 1:4286560/1‑71 (MQ=255) ttACGCCATCTGTTCTGGTCAGATAAACAGACTGAGGCTTATAAACTTCTGTTCAATTTCGTGAATAACCa < 1:1181276/71‑1 (MQ=255) ttACGCCATCTGTTCTGGTCAGATAAACAGACTGAGGCTTATAAACTTCTGTTCAATTTCGTGAATAACCa < 1:130695/71‑1 (MQ=255) cATCTGTTCTGGTCAGATAAACAGACTGAGGCTTATAAACTTCTGTTCAATTTCGTAAATAACCAGACTgg < 1:110174/71‑1 (MQ=255) aTCTGTTCTGGTCAGATAAACAGACTGAGGCTTATAAACTTCTGTTCAATTTCGTGAATAACCAGACTGGc < 1:4228433/71‑1 (MQ=255) aTCTGTTCTGGTCAGATAAACAGACTGAGGCTTATAAACTTCTGTTCAATTTCGTGAATAACCAGACTGGc < 1:4233536/71‑1 (MQ=255) aTCTGTTCTGGTCAGATAAACAGACTGAGGCTTATAAACTTCTGTTCAATTTCGTGAATAACCAGACTGGc < 1:1312154/71‑1 (MQ=255) cTGTTCTGGTCAGATAAACAGACTGAGGCTTATAAACTTCAGTTCAATTTCGTGAATAACCAGACTGGCaa > 1:4263959/1‑71 (MQ=255) tGTTCTGGTCAGATAAACAGACTGAGGCTTATAAACTTCTGTTCAATTTCGTGAATAACCAGACTGGc > 1:2953097/1‑68 (MQ=255) cTGGTCAGATAAACAGACTGAGGCTTATAAACTTCTGTTCAATTTCGTGAATAACCAGACTGGCAACATc < 1:4203722/70‑1 (MQ=255) cTGGTCAGATAAACAGACTGAGGCTTATAAACTTCTGTTCAATTTCGTGAATAACCAGACTGGCAACATc < 1:3736416/70‑1 (MQ=255) cTGGTCAGATAAACAGACTGAGGCTTATAAACTTCTGTTCAATTTCGTGAATAACCAGACTGGCAACATc < 1:3537273/70‑1 (MQ=255) gTCAGATAAACAGACTGAGGCTTATAAACTTCTGTTCAATATCGTGAATAACCAGACTGGCAACATCAACg > 1:2879971/1‑71 (MQ=255) tCAGATAAACAGACTGAGGCTTATAAACTTCTGTTCAATTTCGTGAATAACCAGACTGGCAACATc < 1:1593826/66‑1 (MQ=255) tCAGATAAACAGACTGAGGCTTATAAACTTCTGTTCAATTTCGTGAATAACCAGACTGGCAACATc < 1:3207613/66‑1 (MQ=255) tCAGATAAACAGACTGAGGCTTATAAACTTCTGTTCAATTTCGTAAATAAcc < 1:914976/52‑1 (MQ=255) aGATAAACAGACTGAGGCTTATAAACTTCTGTTCAATTTCGTGAATAACCAGACTGGCAACATCAACGcc < 1:1260782/70‑1 (MQ=255) tAAACAGACTGAGGCTTATAAACTTCTGTTCAATTTCGTGAATAACCAGACTGGCAACATCAACGCCTCAg < 1:3632422/71‑1 (MQ=255) tAAACAGACTGAGGCTTATAAACTTCTGTTCAATTTCGTAAATAACCAGACTGGCAACATCAACGCCTCAg < 1:3417533/71‑1 (MQ=255) aaaCAGACTGAGGCTTATAAACTTCTGTTCAATTTCGTAAATAACCAGACTGGCAACATCAACGCCTCAg > 1:1273233/1‑70 (MQ=255) aaaCAGACTGAGGCTTATAAACTTCTGTTCAATTTCGTAAATAACCAGACTGGCAACATCAACGCCTCAg > 1:2888874/1‑70 (MQ=255) aCAGACTGAGGCTTATAAACTTCTGTTCAATTTCGTGAATAACCAGACTGGCAACa > 1:2410985/1‑56 (MQ=255) aCAGACTGAGGCTTATAAACTTCTGTTCAATTTCGTAAATAACCAGAc < 1:2166498/48‑1 (MQ=255) aCAGACTGAGGCTTATAAACTTCTGTTCAATTTCGTAAATAACCAGAc < 1:3669643/48‑1 (MQ=255) aGACTGAGGCTTATAAACTTCTGTTCAATTTCGTGAATAACCAGACTgg > 1:714636/1‑49 (MQ=255) aGACTGAGGCTTATAAACTTCTGTTCAATTTCGTGAATAACCAGACTGGCAACATCAACGCCTCAGAATAc > 1:1025582/1‑71 (MQ=255) gACTGAGGCTTATAAACTTCTGTTCAATTTCGTGAATAACCAGAc > 1:2120597/1‑45 (MQ=255) cTGAGGCTTATAAACTTCTTTTCAATTTCGTAAATAACCAGACTGGCAACATCAACGCCTCAGAATACttt < 1:158327/71‑1 (MQ=255) tGAGGCTTATAAACTTCTGTTCAATTTCGTGAATaa > 1:1723243/1‑36 (MQ=255) tGAGGCTTATAAACTTCTGTTCAATTCCGTGAATaa < 1:757896/36‑1 (MQ=255) aGGCTTATAAACTTCTGTTCAATTTCGTGAATAACCAGACTGGc < 1:2025423/44‑1 (MQ=255) ggCTTATAAACTTCTGTTCAATTTCGTGAATAACCAGACTGGCAACATCAACGc > 1:2001665/1‑54 (MQ=255) gCTTATAAACTTCTGTTCAATTTCGTGAATAACCAGACTGGCAACATCAACGCCTCAGAATACTTTACTgg > 1:286485/1‑71 (MQ=255) tataAACTTCTGTTCAATTTCGTGAATAACCAGACTGGCAACATCAACGCCTCAGAATACTTTACTGGGGc > 1:2350032/1‑71 (MQ=255) ataAACTTCTGTTCAATTTCGTGAATAACCAGACTGGCAACATCaa > 1:422135/1‑46 (MQ=255) ataAACTTCTGTTCAATTTCGTAAATAACCAGACTGGCAACATCAACTCCTCAGAATACt > 1:235809/1‑60 (MQ=255) taAACTTCTGTTCAATTTCGTGAATAACCAGACTGGCaa > 1:2402484/1‑39 (MQ=255) taAACTTCTGTTCAATTTCGTGAATAACCAGACTGGCAACATCAACGCCTCa > 1:1337340/1‑52 (MQ=255) taAACTTCTGTTCAATTTCGTGAATAACCAGACTGGCAACATCAACGCCTCAGAATACTTTACTg > 1:3315072/1‑65 (MQ=255) aaaCTTCTGTTCAATTTCGTGAATAACCAGACTGGCAACATCaa > 1:217949/1‑44 (MQ=255) aaaCTTCTGTTCAATTTCGTGAATAACCAGACTGGCAACATCAACGCCTCAGAATACTTTACTg < 1:416193/64‑1 (MQ=255) ttCTGTTCAATTTCGTGAATAACCAGACTGGCAACATCAACGc > 1:3010307/1‑43 (MQ=255) ttCTGTTCAATTTCGTAAATAACCAGACTGGCAACATCaa > 1:1439605/1‑40 (MQ=255) tCTGTTCAATTTCGTGAATAACCAGACTGGCAACATCAACGCCTCAGAATACTTTa > 1:857182/1‑56 (MQ=255) cTGTTGAATTTCGTGAATAACCAGACTGGCAACATCAACGCCTCAGAATACTTTACTGGGGCTATCAAc > 1:1960610/1‑69 (MQ=255) cTGTTCAATTTCGTAAATAACCAGACTGGCAACATCAACGCCTCAGAATACTTTACTGGGGCTATCAAc < 1:4417807/69‑1 (MQ=255) gTTCAATTTCGTGAATAACCAGACTGGCAACATCAACGCCTCAGAATACtt > 1:2458242/1‑51 (MQ=255) ttCAATTTCGTGAATAACCAGACTGGCAACATCAACGCCTCAGAATACTTTACTGGGGCTATCAACga < 1:1608828/68‑1 (MQ=255) cAATTTCGTAAATAACCAGACTGGCAACATCAACGCCTCAGAATACTTTACTGGGGCTATCAACGAGAAt > 1:1877988/1‑70 (MQ=255) aaTTTCGTGAATAACCAGACTGGCAACATCAACGCCTCAGAATACTTTACTGGGGCTATCAACGAGAATga < 1:3832651/71‑1 (MQ=255) aaTTTCGTGAATAACCAGACTGGCAACATCAACGCCTCAGAATACTTTACTGGGGCTATCAACGAGAATga < 1:1638989/71‑1 (MQ=255) aTTTCGTGAATAACCAGACTGGCAACATCAACGCCTCAGAATACTTTACTGGGGCTATCAACGAGAATgag < 1:1235608/71‑1 (MQ=255) tttCGTGAATAACCAGACTGGCAACATCAACGCCTCAGAATACTTTACTg < 1:4600835/50‑1 (MQ=255) ttCGTGAATAACCAGACTGGCAACATCAACGCCTc > 1:1212484/1‑35 (MQ=255) ttCGTAAATAACCAGACTGGCAACATCAACGCCTCAGAATACTTTACTGGGGCTAt > 1:2182764/1‑56 (MQ=255) tGAATAACCAGACTGGCAACATCAACGCCTCAGAATACTTTACTGGGGCTATCAACGAGAATGAGAGAGaa < 1:4348569/71‑1 (MQ=255) gAATAACCAGACTGGCAACATCAACGCCTCAGAATACt > 1:2457440/1‑38 (MQ=255) | GGAGACGTTACGCCATCTGTTCTGGTCAGATAAACAGACTGAGGCTTATAAACTTCTGTTCAATTTCGTGAATAACCAGACTGGCAACATCAACGCCTCAGAATACTTTACTGGGGCTATCAACGAGAATGAGAGAGAA > W3110S.gb/58593‑58731 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |