| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 71,214 | T→C | 51.1% | F276F (TTT→TTC) | araC → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 71,214 | 0 | T | C | 51.1% | 7.2 / 131.1 | 88 | F276F (TTT→TTC) | araC | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base T (24/19); new base C (19/26); total (43/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
GGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACAA > W3110S.gb/71146‑71284 | ggTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCg > 1:2528592/1‑71 (MQ=255) ttttGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGt > 1:1190881/1‑70 (MQ=255) ttGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGcc > 1:1142817/1‑71 (MQ=255) ttGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGcc > 1:4264195/1‑71 (MQ=255) ttGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGcc > 1:3639008/1‑71 (MQ=255) ttGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGc < 1:2561113/70‑1 (MQ=255) tGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGcc < 1:1894802/70‑1 (MQ=255) gACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCgg > 1:3893690/1‑71 (MQ=255) cGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGtt < 1:2463305/71‑1 (MQ=255) aTCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTtgt < 1:515647/71‑1 (MQ=255) aTCAACTCTATTTCTCGCG‑GTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTtgtg < 1:1211173/71‑1 (MQ=255) aCTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCgg < 1:19709/63‑1 (MQ=255) tctATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTgaaga > 1:2458288/1‑70 (MQ=255) ctATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTgaa > 1:438859/1‑67 (MQ=255) tATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTgaagaa < 1:1419445/69‑1 (MQ=255) tATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTgaagaa < 1:1360350/69‑1 (MQ=255) tATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGaaa > 1:3086791/1‑70 (MQ=255) tATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGaaa > 1:3030251/1‑70 (MQ=255) tATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGaaa > 1:3149851/1‑70 (MQ=255) tttCTCGCGGGTATTTAAATAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGaaaa < 1:563159/69‑1 (MQ=255) tttCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGaaaa < 1:3958265/69‑1 (MQ=255) tttCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAg > 1:4480953/1‑71 (MQ=255) ttCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGt > 1:3502836/1‑71 (MQ=255) tctcGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGt < 1:2687299/70‑1 (MQ=255) tctcGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGt > 1:2275071/1‑70 (MQ=255) ctcGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTg > 1:3038363/1‑70 (MQ=255) gcgTGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAat < 1:3895845/70‑1 (MQ=255) gcgAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAatg < 1:2675538/71‑1 (MQ=255) gcgAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAatg < 1:3578753/71‑1 (MQ=255) ttAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGtt < 1:1930414/45‑1 (MQ=255) tAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGcc > 1:1428562/1‑70 (MQ=255) aaaaaaTGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGtg > 1:4361205/1‑42 (MQ=255) aaaaaTGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTc < 1:1754009/71‑1 (MQ=255) tGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCTGTCAAGtt < 1:2379224/71‑1 (MQ=255) tGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGtt < 1:1050342/71‑1 (MQ=255) gCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGa > 1:68548/1‑51 (MQ=255) cACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTgaaga > 1:2554581/1‑42 (MQ=255) cACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTg < 1:3530426/70‑1 (MQ=255) aCCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGaaaa < 1:1784285/44‑1 (MQ=255) ccGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCa > 1:2817484/1‑71 (MQ=255) cGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAg < 1:1184051/44‑1 (MQ=255) cGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAg < 1:327579/44‑1 (MQ=255) cGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTTAATGATGTAGCCGTCAAGTTGTCAt < 1:3736590/71‑1 (MQ=255) cGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCa > 1:4188276/1‑70 (MQ=255) cGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGa < 1:2527642/47‑1 (MQ=255) cGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCAt < 1:1086833/71‑1 (MQ=255) ggggCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCAt > 1:1862494/1‑70 (MQ=255) gggCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTg > 1:938993/1‑44 (MQ=255) gggCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATaa > 1:1468122/1‑71 (MQ=255) gggCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGaaa < 1:1455348/39‑1 (MQ=255) gggCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGaaa > 1:1269222/1‑39 (MQ=255) gggCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATaa < 1:3602746/71‑1 (MQ=255) ggCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAAt < 1:3892948/71‑1 (MQ=255) ggCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTc > 1:2722721/1‑66 (MQ=255) ggCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAAa < 1:368998‑M1/71‑2 (MQ=255) ccAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTg < 1:43006/71‑1 (MQ=255) ccAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGaaaaa < 1:2087049/38‑1 (MQ=255) ccAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAAagg < 1:4577005‑M1/71‑4 (MQ=255) cAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTgg > 1:2700420/1‑71 (MQ=255) cAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTgg > 1:1055326/1‑71 (MQ=255) aGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTgg > 1:41173/1‑70 (MQ=255) aGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGt < 1:963422/38‑1 (MQ=255) aGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAAagggt > 1:3601592‑M1/1‑66 (MQ=255) gCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAatgat < 1:1567908/44‑1 (MQ=255) gCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAatg < 1:815351/42‑1 (MQ=255) cccGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATaa < 1:3972539/64‑1 (MQ=255) cccGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAAagggtcc > 1:40117‑M1/1‑64 (MQ=255) ccGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAAagggtccc > 1:4594555‑M1/1‑63 (MQ=255) cgagcgagTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAAc > 1:1189812/1‑70 (MQ=255) cgagcgagTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAAc > 1:88110/1‑70 (MQ=255) cgagcgagTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACg < 1:3387115/71‑1 (MQ=255) cgagcgagTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAg > 1:3540285/1‑53 (MQ=255) cgagcgagTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAAagggtccc > 1:3476460‑M1/1‑62 (MQ=255) cgagcgacTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAAc > 1:409047/1‑70 (MQ=255) gagcgagTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGt < 1:1549478/66‑1 (MQ=255) agcgagTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGaa > 1:3617069/1‑71 (MQ=255) agcgagTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGaa < 1:1545164/71‑1 (MQ=255) gcgagTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTc < 1:4345889/55‑1 (MQ=255) gcgagTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAAagggtccctaag > 1:1646799‑M1/1‑59 (MQ=255) cgagTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTc < 1:2447351/46‑1 (MQ=255) cgagTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAAagggtccctaagt < 1:1151462‑M1/71‑14 (MQ=255) cgagTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAAag > 1:1621110‑M1/1‑58 (MQ=255) gagTTCCGTGCCGGTTGTGAAGAAAAAGTGAatgat < 1:1028980/36‑1 (MQ=255) agTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGcc > 1:1500478/1‑41 (MQ=255) agTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGc > 1:526623/1‑40 (MQ=255) gTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGa > 1:2144731/1‑71 (MQ=255) gTTCCGTGCCGGTTGTGAAGAAAAAGTGAatgatg < 1:3141213/35‑1 (MQ=255) gTTCCGTGCCGGTTGTGAAG‑AAAAGTGAATGATGTAGCCGTCAAGTTGTCATa < 1:672348/53‑1 (MQ=255) tttCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCaa < 1:2881378/44‑1 (MQ=255) tttCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTc < 1:1048387/50‑1 (MQ=255) tCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACaa > 1:4095204/1‑71 (MQ=255) tCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACaa > 1:1412089/1‑71 (MQ=255) | GGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACAA > W3110S.gb/71146‑71284 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |