| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,458,921 | T→C | 13.7% | K6K (AAA→AAG) | nusG ← | transcription termination factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,458,921 | 0 | T | C | 13.7% | 128.1 / 13.5 | 51 | K6K (AAA→AAG) | nusG | transcription termination factor |
| Reads supporting (aligned to +/- strand): ref base T (20/24); new base C (4/3); total (24/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01 | |||||||||||
ATGCTCACGCAGCGACGTTGCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGAACCAGAATACCATC > W3110S.gb/3458852‑3458990 | atGCTCACGCAGCGACGTTGCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCtttt > 1:1937981/1‑71 (MQ=255) gCTCACGCAGCGACGTTGCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCtttt > 1:2736813/1‑69 (MQ=255) cTCACGCAGCGACGTTGCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCtttt < 1:4111785/68‑1 (MQ=255) cTCACGCAGCGACGTTGCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAg < 1:2199785/71‑1 (MQ=255) cAGCGACGTTGCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCtt < 1:1088162/71‑1 (MQ=255) cAGCGACGTTGCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCtt < 1:4174209/71‑1 (MQ=255) aGCGACGTTGCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCtt > 1:2383455/1‑70 (MQ=255) aGCGACGTTGCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTCTTAGGAGCTTc < 1:1802759/71‑1 (MQ=255) gACGTTGCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTCTTAGGAGCTTCAGa > 1:2021695/1‑71 (MQ=255) aCGTTGCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTa < 1:2142826/59‑1 (MQ=255) cGTTGCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAg > 1:3967818/1‑68 (MQ=255) ttGCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCttttt < 1:4057170/55‑1 (MQ=255) ttGCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACa < 1:2200804/69‑1 (MQ=255) gCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACAtct > 1:3334920/1‑70 (MQ=255) gCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTCTTAGGAGCTTCAGACAtct < 1:2554253/70‑1 (MQ=255) tACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCttt > 1:1643589/1‑49 (MQ=255) gcgGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAAc < 1:3078403/71‑1 (MQ=255) cTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAg < 1:2603601/45‑1 (MQ=255) cTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACAt < 1:4045785/58‑1 (MQ=255) cTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAg < 1:4172144/63‑1 (MQ=255) cTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAg < 1:3954427/71‑1 (MQ=255) tCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGc > 1:4366803/1‑47 (MQ=255) tCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCt < 1:868834/66‑1 (MQ=255) cAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCa > 1:2294400/1‑50 (MQ=255) cAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACAtctc > 1:341332/1‑58 (MQ=255) cAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCa > 1:886357/1‑59 (MQ=255) cAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGc > 1:2435274/1‑70 (MQ=255) aaaaCCGGAAAACGCCTGAACGACGTACCAGCGCTTCTTAGGAGCTTCAGACATCTCAGAACCTCAGGc > 1:3306151/1‑69 (MQ=255) aaaCCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTa > 1:3609024/1‑39 (MQ=255) aaaCCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACAt < 1:568812/53‑1 (MQ=255) aaaCCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAg < 1:3390113/71‑1 (MQ=255) aaCCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCt < 1:402457/44‑1 (MQ=255) aaCCGGAAAACGCCTGAACGACGTACCAGCGCTTCTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGt < 1:4058230/71‑1 (MQ=255) aCCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGt > 1:1797637/1‑70 (MQ=255) cGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGAt < 1:2727220/71‑1 (MQ=255) ggAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTc > 1:530000/1‑59 (MQ=255) ggAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATa < 1:1231367/71‑1 (MQ=255) gAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATa > 1:1384795/1‑70 (MQ=255) aaaCGCCTGAACGACGTACCAGCGCTTTTTAGGAGCtt < 1:2886015/38‑1 (MQ=255) aaaCGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAg > 1:3149040/1‑71 (MQ=255) aaaCGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACATCAGGCCAGTGATAAAg < 1:79474/71‑1 (MQ=255) aaCGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAg > 1:82007/1‑70 (MQ=255) ccTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATAc > 1:321555/1‑71 (MQ=255) cTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATAcc < 1:1063934/71‑1 (MQ=255) gAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAg > 1:3552022/1‑71 (MQ=255) aaCGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCa < 1:17811/69‑1 (MQ=255) acgacgTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGc < 1:2593070/71‑1 (MQ=255) acgacgTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGc < 1:4038577/71‑1 (MQ=255) acgacgTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGc > 1:749837/1‑71 (MQ=255) gacgTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAg > 1:63682/1‑46 (MQ=255) cgTACCAGCGCTTCTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGAAc > 1:948618/1‑71 (MQ=255) gcgcTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATAcc < 1:2589351/56‑1 (MQ=255) gcgcTTCTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGAACCAGAATa > 1:4502981/1‑71 (MQ=255) cgcTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGAACCAGAATAc < 1:453473/71‑1 (MQ=255) gcTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGaa > 1:667837/1‑61 (MQ=255) cTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGAACCAGaa > 1:4041921/1‑66 (MQ=255) tttttAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCa > 1:1852015/1‑53 (MQ=255) tttAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGAACCAGAATACCAt < 1:2110969/69‑1 (MQ=255) tttAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGAACCAGAATACCATc > 1:3185228/1‑70 (MQ=255) tttAGGAGCTTCAGACATCTCAGAACATCAGGCCAGTGATAAAGGATAACAGGCGAACCAGaa > 1:3056412/1‑63 (MQ=255) | ATGCTCACGCAGCGACGTTGCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGAACCAGAATACCATC > W3110S.gb/3458852‑3458990 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |