| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,472,611 | G→A | 15.5% | G144G (GGC→GGT) | btuB ← | vitamin B12/cobalamin outer membrane transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,472,611 | 0 | G | A | 15.5% | 113.7 / 12.7 | 45 | G144G (GGC→GGT) | btuB | vitamin B12/cobalamin outer membrane transporter |
| Reads supporting (aligned to +/- strand): ref base G (17/21); new base A (3/4); total (20/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.77e-01 | |||||||||||
TTGCTTCCCCACCCTGCTGAAATTTCCGTTCCGGGTTCATCGCGCGTCGTGATGATATTCACCACCCCGCCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGATATATTCAACACGCTGGACAAGCGCAA > W3110S.gb/3472543‑3472681 | ttGCTTCCCCACCCTGCTGAAATTTCCGTTCCGGGTTCATCGCGCGTCGTGATGATATTCACCACCCCGcc < 1:3802195/71‑1 (MQ=255) tgctgAAATTTCCGTTCCGGGTTCATCGCGCGTCGTGATGATATTCACCACCCCGCCTATTGCATCGGAAc > 1:2754331/1‑71 (MQ=255) gAAATTTCCGTTCCGGGTTCATCGCGCGTCGTGATGATATTCACCACCCCGCCTATTGCATCGGAACCAt > 1:3576507/1‑70 (MQ=255) gAAATTTCCGTTCCGGGTTCATCGCGCGTCGTGATGATATTCACCACCCCGCCTATTGCATCGGAACCAt > 1:175722/1‑70 (MQ=255) aaaTTTCCGTTCCGGGTTCATCGCGCGTCGTGATGATATTCACCACCCCACCTATTGCATCGGAACCATa < 1:3161077/70‑1 (MQ=255) tttCCGTTCCGGGTTCATCGCGCGTCGTGATGATATTCACCACCCCGCCTATTGCATCGGAACCATAAACa < 1:1877741/71‑1 (MQ=255) tttCCGTTCCGGGTTCATCGCGCGTCGTGATGATATTCACCACCCCGCCTATTGCATCGGAACCATAAACa < 1:740064/71‑1 (MQ=255) ccgttccgGGTTCATCGCGCGTCGTGATGATATTCACCACCCCGCCTATTGCATCGGAACCATAAACAg < 1:4267718/69‑1 (MQ=255) cgttccgGGTTCATCGCGCGTCGTGATGATATTCACCACCCCGCCTATTGCAt > 1:1868634/1‑53 (MQ=255) cgttccgGGTTCATCGCGCGTCGTGATGATATTCACCACCCCGCCTATTGCATCGGAACCATAAACAGCg < 1:3682337/70‑1 (MQ=255) ttccgGGTTCATCGCGCGTCGTGATGATATTCACCACCCCGCCTATTGCATCGGAACCATAAACAGCg < 1:333277/68‑1 (MQ=255) ttccgGGTTCATCGCGCGTCGTGATGATATTCACCACCCCGCCTATTGCATCGGAACCATAAACAGCGGAg < 1:1928278/71‑1 (MQ=255) tccgGGTTCATCGCGCGTCGTGATGATATTCACCACCCCGCCTa < 1:2036961/44‑1 (MQ=255) tccgGGTTCATCGCGCGTCGTGATGATATTCACCACCCCGCCTAt > 1:1165898/1‑45 (MQ=255) ccgGGTTCATCGCGCGTCGTGATGATATTCACCACCCCGCCTATTGCATCGGAACCATa < 1:2922426/59‑1 (MQ=255) ccgGGTTCATCGCGCGTCGTGATGATATTCACCACCCCACCTATTGCATCGGAACCATAAACAGCGGAgcg > 1:4472316/1‑71 (MQ=255) cgGGTTCATCGCGCGTCGTGATGATATTCACCACCCCGCCTATTGCATCGGAACCATAAACAGCGGAgcg < 1:4384579/70‑1 (MQ=255) cgGGTTCATCGCGCGTCGTGATGATATTCACCACCCCACCTATTGCATCGGAACCATAAACAGCGGAgcgc < 1:2813526/71‑1 (MQ=255) gggTTCATCGCGCGTCGTGATGATATTCACCACCCCGCCTATTGCATCGGAACCATaa > 1:2049283/1‑58 (MQ=255) gggCTCATCGCGCGTCGTGATGATATTCACCACCCCGCCTATTGCATCGGAACCATAAACAGCGGAGcgcg < 1:3695200/71‑1 (MQ=255) ttCATCGCGCGTCGTGATGATATTCACCACCCCGCCTATTGCATCGGAACCATAAACAGCGGAGcgcg < 1:2332830/68‑1 (MQ=255) tCATCGCGCGTCGTGATGATATTCACCACCCCGCCTATTGCATCGGAACCATAAACAGCGGAGCGCGGcc > 1:155958/1‑70 (MQ=255) cgcgTCGTGATGATATTCACCACCCCACCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGat < 1:2438413/71‑1 (MQ=255) gcgTCGTGATGATATTCACCACCCCGCCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGata > 1:3317586/1‑71 (MQ=255) cgtcgtGATGATATTCACCACCCCGCCTATTGCATCGGAACCATAAACAGCGGAgc > 1:387711/1‑56 (MQ=255) gtcgtGATGATATTCACCACCCCGCCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGatat < 1:2261983/70‑1 (MQ=255) cgtGATGATATTCACCACCCCGCCTATTGCATCGGAACCATAAACAGCGGAg < 1:972212/52‑1 (MQ=255) cgtGATGATATTCAACACCCCGCCTATTGCATCGGAACCATAAACAGCg > 1:911255/1‑49 (MQ=255) gatgatATTCACCACCCCGCCTATTGCATCGGAACCATaaa > 1:347252/1‑41 (MQ=255) atgatATTCACCACCCCGCCTATTGCATCGGAACCATAAACAGCGGAg > 1:1750751/1‑48 (MQ=255) atgatATTCACCACCCCGCCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGATATATTCaa > 1:4346298/1‑70 (MQ=255) atgatATTCACCACCCCGCCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGATATATTCaa > 1:3244239/1‑70 (MQ=255) tgatATTCACCACCCCACCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGATATATTCAaca > 1:3475371/1‑71 (MQ=255) atatTCACCACCCCGCCTATTGCATCGGAACCATAAACAGCGGAgcg < 1:2603604/47‑1 (MQ=255) atatTCACCACCCCGCCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGATATATTCAacac < 1:3764373/70‑1 (MQ=255) tatTCACCACCCCGCCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGATATATTCAACACGc < 1:195642/71‑1 (MQ=255) tatTCACCACCCCACCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGATATATTCAACACGc > 1:3091842/1‑71 (MQ=255) atTCACCACCCCGCCTATTGCATCGGAACCATAAACAGc > 1:4550399/1‑39 (MQ=255) ttCACCACCCCACCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGATATATTCAACACGCTg < 1:2869519/71‑1 (MQ=255) accaccCCGCCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGATATATTCAACACGCTGGAc < 1:2095846/71‑1 (MQ=255) accaccCCGCCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGATATATTCAACACGCTGGAc < 1:609372/71‑1 (MQ=255) caccCCGCCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGATATATTCAACACGCTGGACaa < 1:2915821/71‑1 (MQ=255) caccCCGCCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGATATATTCAACACGCTGGACaa < 1:4239146/71‑1 (MQ=255) ccGCATATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGATATATTCAACACGCTGGACAAgcg > 1:2931004/1‑70 (MQ=255) cGCCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGATATATTCAACACGCTGGa > 1:4139522/1‑63 (MQ=255) gCCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGATATATTCAACACGCTGGACAAGCGCaa > 1:4097727/1‑71 (MQ=255) | TTGCTTCCCCACCCTGCTGAAATTTCCGTTCCGGGTTCATCGCGCGTCGTGATGATATTCACCACCCCGCCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGATATATTCAACACGCTGGACAAGCGCAA > W3110S.gb/3472543‑3472681 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |