| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,521,572 | G→A | 14.1% | L157L (CTG→CTA) | glpX → | fructose 1,6‑bisphosphatase II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,521,572 | 0 | G | A | 14.1% | 174.4 / 17.5 | 64 | L157L (CTG→CTA) | glpX | fructose 1,6‑bisphosphatase II |
| Reads supporting (aligned to +/- strand): ref base G (35/20); new base A (4/5); total (39/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.96e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
TGAACCTGCCGCTGGCGGATAACCTGCGCAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAATGCAGCAACTCGGCGTA > W3110S.gb/3521505‑3521638 | tGAACCTGCCGCTGGCGGATAACCTGCGCAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACg > 1:4117628/1‑71 (MQ=255) aaCCTGCCGCTGGCGGATAACCTGCGCAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACgg > 1:2935567/1‑70 (MQ=255) aaCCTGCCGCTGGCGGATAACCTGCGCAATGTAGCGGCGGCGCTCGGAAAACCGTTGAGCGAACTGACgg > 1:4122443/1‑70 (MQ=255) aCCTGCCGCTGGCGGATAACCTGCGCAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTAACGGTa < 1:3933328/71‑1 (MQ=255) cTGCCGCTGGCGGATAACCTGCGCAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTAACGGTAAc < 1:2486905/71‑1 (MQ=255) cGCTGGCGGATAACCTGCGCAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACGGTAACGAtt > 1:1495199/1‑71 (MQ=255) cTGGCGGATAACCTGCGCAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCt < 1:3477276/71‑1 (MQ=255) ggCGGATAACCTGCGCAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTgg > 1:794539/1‑71 (MQ=255) gCGGATAACCTGCGCAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTgg > 1:4440279/1‑70 (MQ=255) ggATAACCTGCGCAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTa < 1:2646022/71‑1 (MQ=255) ggATAACCTGCGCAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTa < 1:290807/71‑1 (MQ=255) gATAACCTGCGCAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTaa < 1:2468480/71‑1 (MQ=255) gATAACCTGCGCAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTaa < 1:1821530/71‑1 (MQ=255) aaCCTGCGCAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTAACGGTAACGATTCTg > 1:96570/1‑63 (MQ=255) aCCTGCGCAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAAcca > 1:1477781/1‑71 (MQ=255) tGCGCAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAAccacgc > 1:263074/1‑71 (MQ=255) tGCGCAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAAccacgc > 1:111196/1‑71 (MQ=255) gcgcAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACGGTAACGAtt > 1:214103/1‑55 (MQ=255) gcgcAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTAACGGTAAc < 1:4402551/51‑1 (MQ=255) cgcAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAAccacgcc < 1:1104704/70‑1 (MQ=255) gcAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAAccacgccac < 1:2973455/71‑1 (MQ=255) tGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCt > 1:154530/1‑55 (MQ=255) tGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATg > 1:2664155/1‑71 (MQ=255) gTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGAc > 1:1387786/1‑38 (MQ=255) gTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAAcca > 1:1176647/1‑60 (MQ=255) gcggcggcgCTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGt > 1:1476100/1‑71 (MQ=255) cggcggcgCTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTaa < 1:1492735/52‑1 (MQ=255) cggcggcgCTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGtt < 1:4428286/71‑1 (MQ=255) ggcggcgCTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCt < 1:1420346/45‑1 (MQ=255) gcggcgCTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTaaa > 1:4332671/1‑51 (MQ=255) cggcgCTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTAt > 1:684268/1‑70 (MQ=255) ggcgCTCGGCAAACCGTTGAGCGAACTGCCGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCg > 1:2959971/1‑71 (MQ=255) gcgcTCGGCAAACCGTTGAGCGAACTAACGGTAACGATTCTGGCTAAACCACGCCACGATg < 1:3486864/61‑1 (MQ=255) cgcTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCt > 1:3465992/1‑71 (MQ=255) gcTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTg > 1:893384/1‑40 (MQ=255) gcTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCg < 1:3199221/68‑1 (MQ=255) gcTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCg < 1:3979360/68‑1 (MQ=255) gcTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCt > 1:4436518/1‑70 (MQ=255) gcTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCt > 1:1996117/1‑70 (MQ=255) gcTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTg < 1:3249583/71‑1 (MQ=255) cTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGa < 1:524300/71‑1 (MQ=255) tCGGCAAACCGTTGAGCGAACTAACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGa > 1:452432/1‑70 (MQ=255) ggCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAAt > 1:1400573/1‑71 (MQ=255) ggCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAAt > 1:3064762/1‑71 (MQ=255) ggCAAACCGTTGAGCGAACTAACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGaaa > 1:3845019/1‑70 (MQ=255) gCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAATg < 1:85417/71‑1 (MQ=255) cAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAATg > 1:1043659/1‑70 (MQ=255) aCCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAATgcag > 1:4330867/1‑70 (MQ=255) ccGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAATgcagca < 1:4051727/71‑1 (MQ=255) cGTTGAGCGAACTGACGGTAACGATTCTGGCTAAAccac > 1:1387208/1‑39 (MQ=255) ttGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTa < 1:2796129/52‑1 (MQ=255) tGAGCGAACTAACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAATGCAGCAACTc > 1:4178351/1‑71 (MQ=255) gAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCg > 1:4411042/1‑47 (MQ=255) gAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATGGCTGAAATGCAGCAACTCg > 1:4152280/1‑71 (MQ=255) gAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCg > 1:3376046/1‑53 (MQ=255) gAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCg > 1:2747611/1‑53 (MQ=255) aGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAATGCAGCAACTCg < 1:1921430/70‑1 (MQ=255) gCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAATGCAGCAACTCGGc < 1:692506/71‑1 (MQ=255) gCGAACTAACGGTAACGATTCTGGCTAAAccacgccac < 1:3795895/38‑1 (MQ=255) gAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAATGCAGCAACTCGGCg > 1:4284084/1‑70 (MQ=255) gAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAATGCAGCAACTCGGCg > 1:4067976/1‑70 (MQ=255) aaCTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAATGCAGCAACTCGGCGTa > 1:134244/1‑71 (MQ=255) aaCTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAATGCAGCAACTCGGCGTa < 1:3662380/71‑1 (MQ=255) cTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAATGCAGCAACTCGGCGTa > 1:2631815/1‑69 (MQ=255) | TGAACCTGCCGCTGGCGGATAACCTGCGCAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAATGCAGCAACTCGGCGTA > W3110S.gb/3521505‑3521638 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |