| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,533,883 | T→C | 8.2% | intergenic (‑24/+125) | yiiM ← / ← kdgT | conserved hypothetical protein/2‑keto‑3‑deoxy‑D‑gluconate transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,533,883 | 0 | T | C | 8.2% | 168.7 / 6.8 | 61 | intergenic (‑24/+125) | yiiM/kdgT | conserved hypothetical protein/2‑keto‑3‑deoxy‑D‑gluconate transporter |
| Reads supporting (aligned to +/- strand): ref base T (29/27); new base C (2/3); total (31/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
GATAAGCCTGAATCTTGCCTGTGTATACATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCGGTAAGTATCCAGGCAGGAACTC > W3110S.gb/3533814‑3533951 | gATAAGCCTGAATCTTGCCTGTGTATACATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTc < 1:1443319/71‑1 (MQ=255) tAAGCCTGAATCTTGCCTGTGTATACATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAAttt < 1:4304661/68‑1 (MQ=255) tAAGCCTGAATCTTGCCTGTGTATACATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTccc < 1:3418220/71‑1 (MQ=255) tAAGCCTGAATCTTGCCTGTGTATACATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTccc > 1:4519588/1‑71 (MQ=255) aaGCCTGAATCTTGCCTGTGTATACATCACCCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTccc > 1:1068702/1‑70 (MQ=255) aGCCTGAATCTTGCCTGTGTATACATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAAttt > 1:1222365/1‑66 (MQ=255) aGCCTGAATCTTGCCTGTGTATACATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTcccc < 1:132765/70‑1 (MQ=255) aGCCTGAATCTTGCCTGTGTATACATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTcccc < 1:2978461/70‑1 (MQ=255) aGCCTGAATCTTGCCTGTGTATACATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTCCCCCa > 1:1650069/1‑71 (MQ=255) gCCTGAATCTTGCCTGTGTATACATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCAt > 1:3676580/1‑71 (MQ=255) cTGAATCTTGCCTGTGTATACATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTa < 1:712341/71‑1 (MQ=255) cTGAATCTTGCCTGTGTATACATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTa < 1:3014583/71‑1 (MQ=255) tGAATCTTGCCTGTGTATACATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAAttt > 1:3443771/1‑62 (MQ=255) gAATCTTGCCTGTGTATACATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGa > 1:3332313/1‑71 (MQ=255) aaTCTTGCCTGTGTATACATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCa > 1:1631539/1‑65 (MQ=255) aTCTTGCCTGTGTATACATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCAt > 1:2598405/1‑65 (MQ=255) gCCTGTGTATACATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCatat > 1:1084011/1‑70 (MQ=255) tgtgTATACATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCatat < 1:4024308/67‑1 (MQ=255) gtgtATACATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTCCCCCATTAGACCAtatttat > 1:2719858/1‑70 (MQ=255) tgtATACATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCAtatttat < 1:3533081/69‑1 (MQ=255) tgtATACATCAACCGGATATCCCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATatttattt < 1:2849888/71‑1 (MQ=255) ataCATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGAcc > 1:4299541/1‑58 (MQ=255) ataCATCAACCCGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGAcc > 1:2804281/1‑58 (MQ=255) aCATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATg > 1:936554/1‑70 (MQ=255) cATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCAtattt < 1:3561238/61‑1 (MQ=255) cATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGt > 1:3176170/1‑70 (MQ=255) aTCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATAtttatttt > 1:2075249/1‑65 (MQ=255) tCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAg < 1:3393894/71‑1 (MQ=255) tCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTCCCCCATTAGACCATATTTATTTTATGTCAg < 1:3125892/71‑1 (MQ=255) aaCCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGc > 1:3304724/1‑71 (MQ=255) aaCCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGc > 1:3218439/1‑71 (MQ=255) aCCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGc > 1:3648854/1‑70 (MQ=255) aCCGGATATCGCATCCGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCg < 1:2739697/71‑1 (MQ=255) cGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTg < 1:3524653/71‑1 (MQ=255) cGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTg < 1:3722972/71‑1 (MQ=255) ggATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATAtttatttta > 1:2610662/1‑59 (MQ=255) tCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGtt < 1:1664259/70‑1 (MQ=255) cATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCata < 1:1879923/42‑1 (MQ=255) aTCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTcgc < 1:4501283/71‑1 (MQ=255) tGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCgg > 1:1443209/1‑70 (MQ=255) tGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCgg > 1:1182330/1‑70 (MQ=255) tGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCgg > 1:1500771/1‑70 (MQ=255) gTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGtt < 1:1444489/62‑1 (MQ=255) ttCCCTCTCCACGAGAAATTTCCCCATTAGACCAtatt < 1:373503/38‑1 (MQ=255) ttCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTcgcg < 1:2390714/67‑1 (MQ=255) ttCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCGGt < 1:717461/69‑1 (MQ=255) tCCCTCTCCACGTGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCGGTAAg > 1:1967328/1‑71 (MQ=255) tCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGt > 1:1632629/1‑47 (MQ=255) cccTCTCCACGAGAAATTTCCCCATTAGACCATatttattt > 1:3433892/1‑41 (MQ=255) cccTCTCCACGAGAAATTTCCCCATTAGACCATAtttattttat > 1:1883678/1‑44 (MQ=255) cccTCTCCACGAGAAATTTCCCCATTAGACCATAtttattttat > 1:2254933/1‑44 (MQ=255) ccTCTCCACGAGAAATTTCCCCATTAGACCAtatttatt < 1:328881/39‑1 (MQ=255) ctctCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCGGt < 1:147245/65‑1 (MQ=255) tctcCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCGGTAAGTATc > 1:1415637/1‑71 (MQ=255) tctcCACGAGAAATTCCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCGGTAAg < 1:2539332/67‑1 (MQ=255) ctcCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCGGTAAGTATc > 1:3966946/1‑70 (MQ=255) ccACGAGAAATTTCCCCATTAGACCATAtttatttt < 1:4599654/36‑1 (MQ=255) aCGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCg > 1:338590/1‑44 (MQ=255) cGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCGGTAAGTATCcagg > 1:4496178/1‑69 (MQ=255) gagaAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCGGTAAGTATCcaggca < 1:2893335/70‑1 (MQ=255) agaAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCGGTAAGTATCcaggcagg < 1:1585642/71‑1 (MQ=255) aaaTTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGttt < 1:1540038/47‑1 (MQ=255) aaTTCCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCGGTAAGTATCCAGGCAGGAAc < 1:2750746/71‑1 (MQ=255) aTTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCGGTAAGTATCCAGGCAGGAACt > 1:1310594/1‑71 (MQ=255) tttCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCGGTAAGTATCcagg < 1:904327/62‑1 (MQ=255) tttCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCGGTAAGTATCCAGGCAGGAACTc < 1:4165430/71‑1 (MQ=255) tttCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCGGTAAGTATCCAGGCAGGAACTc < 1:1879793/71‑1 (MQ=255) ttCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTc > 1:3038262/1‑45 (MQ=255) | GATAAGCCTGAATCTTGCCTGTGTATACATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCGGTAAGTATCCAGGCAGGAACTC > W3110S.gb/3533814‑3533951 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |