| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,584,576 | G→A | 8.9% | P21S (CCA→TCA) | hemN ← | coproporphyrinogen III oxidase, SAM and NAD(P)H dependent, oxygen‑independent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,584,576 | 0 | G | A | 8.9% | 135.8 / 3.8 | 45 | P21S (CCA→TCA) | hemN | coproporphyrinogen III oxidase, SAM and NAD(P)H dependent, oxygen‑independent |
| Reads supporting (aligned to +/- strand): ref base G (19/22); new base A (2/2); total (21/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-01 | |||||||||||
TAAAAACGCCTGTTCGCCGAAGTCTTCTGAAAACTCCAGCGCGGTCGGGTACGAGGTGTATCGTGGCCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAGTCGATTTGCTGTACAGACATGCTCACTCCT > W3110S.gb/3584511‑3584646 | tAAAAACGCCTGTTCGCCGAAGTCTTCTGAAAACTCCAGCGCGGTCGGGTACGAGGTGTATCGTGGCCCgg < 1:1044158/71‑1 (MQ=255)tAAAAACGCCTGTTCGCCGAAGTCTTCTGAAAACTCCAGCGCGGTCGGGTACGAGGTGTATCGTGGCCCg > 1:2744098/1‑70 (MQ=255)tAAAAACGCCTGTTAGCCGAAGTCTTCTGAAAACTCCAGCGCGGTCGGGTACGAGGTGTATCGTGGCCCg > 1:518516/1‑70 (MQ=255) aaaaCGCCTGTTCGCCGAAGTCTTCTGAAAACTCCAGCGCGGTCGGGTACGAGGTGTATCGTGGCCCGGaa > 1:1166437/1‑71 (MQ=255) aCGCCTGTTCGCCGAAGTCTTCTGAAAACTCCAGCGCGGTCGGGTACGAGGTGTATCGTGGCCCGGaa < 1:2931846/68‑1 (MQ=255) gTTCGCCGAAGTCTTCTGAAAACTCCAGCGCGGTCGGGTACGAGGTGTATCGTGGCCCGGAATAGTTatat > 1:1224835/1‑71 (MQ=255) ttCGCCGAAGTCTTCTGAAAACTCCAGCGCGGTCGGGTACGAGGTGTATCGTGGCCCGGAATAGTTatat < 1:1703351/70‑1 (MQ=255) tCGCCGAAGTCTTCTGAAAACTCCAGCGCGGTCGGGTACGAGGTGTATCGTGGc > 1:192380/1‑54 (MQ=255) tCGCCGAAGTCTTCTGAAAACTCCAGCGCGGTCGGGTACGAGGTGTATCGTGGCCCGGAATAGTTATAttt > 1:4363751/1‑71 (MQ=255) gCCGAAGTCTTCTGAAAACTCCAGCGCGGTCGGGTACGAGGTGTATCGTGGCCCGGAATAGTTATATTTCt < 1:1286046/71‑1 (MQ=255) cGAAGTCTTCTGAAAACTCCAGCGCGGTCGGGTACGAGGTGTATCGTgg < 1:1046015/49‑1 (MQ=255) aaGTCTTCTGAAAACTCCAGCGCGGTCGGGTACGAGGTGTATCGTGGCCCGGAATAGTTATATTTCTGGAt > 1:2859910/1‑71 (MQ=255) aaGTCTTCTGAAAACTCCAGCGCGGTCGGGTACGAGGTGTATCGTGGCCCGGAATAGTTATATTTCTGGAt > 1:2501867/1‑71 (MQ=255) aaGTCTTCTGAAAACTCCAGCGCGGTCGGGTACGAGGTGTATCGTGACCCGGAATAGTTATATTTCTGGAt < 1:1238583/71‑1 (MQ=255) gTCTTCTGAAAACTCCAGCGCGGTCGGGTACGAGGTGTATCGTGACCCGGAATAGTTATATTTCTGGATCa < 1:729705/71‑1 (MQ=255) tctGAAAACTCCAGCGCGGTCGGGTACGAGGTGTATCGTGACCCGGAATa > 1:3509103/1‑50 (MQ=255) ctGAAAACTCCAGGGCGGTCGGGTACGAGGTGTATCGTGGCCCGGAATAGTTATATTTCTGGATCAGGGc < 1:2228135/70‑1 (MQ=255) ctGAAAACTCCAGCGCGGTCGGGTACGATGTGTATCGTGGCCCGGAATAGTTATATTTCTGGATCAgg < 1:201848/68‑1 (MQ=255) gAAAACTCCAGCGCGGTCGGGTACGAGGTGTATCGTGGccc > 1:4052320/1‑41 (MQ=255) gAAAACTCCAGCGCGGTCGGGTACGAGGTGTATCGTGGCCCgg < 1:1890699/43‑1 (MQ=255) aaaaCTCCAGCGCGGTCGGGTACGAGGTGTATCGTGGCCCGGAATAGTTATATTTCTGGATCAGGGc > 1:3927397/1‑67 (MQ=255) aaaaCTCCAGCGCGGTCGGGTACGAGGTGTATCGTGGCCCGGAATAGTTATATTTCTGGATCAGGGCCAg < 1:1226142/70‑1 (MQ=255) aaaaCTCCAGCGCGGTCGGGTACGAGGTGTATCGTGGCCCGGAATAGTTATATTTCTGGATCAGGGCCAGa < 1:4581644/71‑1 (MQ=255) aaaCTCCAGCGCGGTCGGGTACGAGGTGTATCGTGGCCCGGAATAGTTATAATTCTGGATCAGGGc > 1:571419/1‑66 (MQ=255) aaCTCCAGCGCGGTCGGGTACGAGGTGTATCGTGGCCCGGAATAGTTATATTTCTGGATCAGGGCCAGATc > 1:2379205/1‑71 (MQ=255) aCTCCAGCGCGGTCGGGTACGAGGTGTATCGTGGCCCGGAATAGTTATAttt < 1:4400610/52‑1 (MQ=255) cTCCAGCGCGGTCGGGTACGAGGTGTATCGTGGCCCGGAATAGTTATATTTCt > 1:1031319/1‑53 (MQ=255) cTCCAGCGCGGTCGGGTACGAGGTGTATCGTGGCCCGGAATAGTTATATTTCTGGATCAGGGCCAGATccc < 1:4226242/71‑1 (MQ=255) tCCAGCGCGGTCGGGTACGAGGTGTATCGTGGCCCGGAATAGTTATATTTc < 1:3423097/51‑1 (MQ=255) cAGCGCGGTCGGGTACGAGGTGTATCGTGGCCCGGAATAGTTATATTTCTGGATCAGGGCCAGATc > 1:1782989/1‑66 (MQ=255) gcgcGGTCGGGTACGAGGTGTATCGTGGCCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAGTCg < 1:3732152/71‑1 (MQ=255) cgcgGTCGGGAACGAGGTGTATCGTGGCCCGGAATa < 1:3354542/36‑1 (MQ=255) tCGGGTACGAGGTGTATCGTGGCCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAGTc < 1:1803597/64‑1 (MQ=255) gggTACGAGGTGTATCGTGGCCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAGTCGATTTGCTg > 1:873951/1‑71 (MQ=255) gggTACGAGGTGTATCGTGACCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAGTCGATTTGCTg > 1:1148558/1‑71 (MQ=255) ggTACGAGGTGTATCGTGGCCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAGTCGATTTGCTGt < 1:21787/71‑1 (MQ=255) tACGAGGTGTATCGTGGCCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAGTCGATTTGCTGTa > 1:553112/1‑70 (MQ=255) cGAGGTGTATCGTGGCCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAGTCGATTTGCTGTACAg < 1:3914337/71‑1 (MQ=255) cGAGGTGTATCGTGGCCAGGAATAGTTATATTTCTGGATGAGGGCCAGATCCCAGTCGATTTGCTGTa > 1:2996466/1‑68 (MQ=255) ggTGTATCGTGGCCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAGTCGATTTGCTGTACAGACa > 1:1549258/1‑71 (MQ=255) ggTGTATCGTGGCCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCAAGTCGATTTGCTGTACAGACa < 1:1755149/71‑1 (MQ=255) tgtATCGTGGCCCGGAATAGTTATATTTCTGGATCAGGGACAGATCCCAGTCGATTTGCTGTACAGACATg < 1:4345649/71‑1 (MQ=255) tCGTGGCCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAGTCGAtt > 1:3976422/1‑52 (MQ=255) tCGTGGCCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAGTCGATTTGCTg < 1:208606/57‑1 (MQ=255) gTGGCCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAGTCGATTTGCTGTACAGACATg < 1:1043173/65‑1 (MQ=255) gTGGCCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAGTCGATTTGCTGTACAGACATGCTCAc < 1:2214028/70‑1 (MQ=255) gCCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAGTCGATTTGCTGTACAGACATGCTCACTCCt < 1:3966139/71‑1 (MQ=255) | TAAAAACGCCTGTTCGCCGAAGTCTTCTGAAAACTCCAGCGCGGTCGGGTACGAGGTGTATCGTGGCCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAGTCGATTTGCTGTACAGACATGCTCACTCCT > W3110S.gb/3584511‑3584646 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |