Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 3,824,368 G→A 100% pseudogene (501/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,824,3680GA100.0% 180.0 / NA 61pseudogene (501/687 nt)rphECK3633:JW3618:b3643; ribonuclease PH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/37);  total (24/37)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GTGGAAAACGGCAAGCTGAAAACCAATCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGAATTGTGAACGGCGAAGCGGTTTGCGATCTGGAATACGTTGAAGACTCTGCCGCAGAGACCGACATGAACG  >  W3110S.gb/3824300‑3824432
                                                                    |                                                                
gtggAAAACGGCAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTaaa                                                                >  1:4207383/1‑71 (MQ=255)
    aaaaCGGCAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACggt                                                            <  1:4312769/71‑2 (MQ=255)
       aCGGCAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACggtg                                                           >  1:438505/1‑67 (MQ=255)
          gCAAGCTGAAAACCAACCCGATGAAAGGTATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGca                                                      <  1:834686/71‑2 (MQ=255)
          gCAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCACAGTTTCTGTCGGTATTGTAAACGGTGAAGca                                                      <  1:1203430/71‑2 (MQ=255)
            aaGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGcaat                                                    <  1:140021/71‑4 (MQ=255)
               cTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGcaat                                                    <  1:1717685/68‑4 (MQ=255)
               cTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTg                                                 >  1:4091924/1‑71 (MQ=255)
                  aaaaCCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTaaa                                                                >  1:2805649/1‑53 (MQ=255)
                   aaaCCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGAt                                             >  1:2765275/1‑71 (MQ=255)
                     aCCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACggt                                                            <  1:3230139/54‑2 (MQ=255)
                       cAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTgg                                         <  1:2354774/71‑1 (MQ=255)
                        aacccGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGa                                        <  1:760545/68‑1 (MQ=255)
                         acccGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGaa                                       <  1:3916880/69‑1 (MQ=255)
                           ccGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACggtg                                                           >  1:1889710/1‑47 (MQ=38)
                           ccGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACgg                                                             <  1:3476162/47‑1 (MQ=39)
                           ccGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGaa                                                         >  1:2769127/1‑51 (MQ=255)
                           ccGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGAt                                             <  1:415317/63‑1 (MQ=255)
                           ccGATGAAAGGGATGGTAGCCGCAGTTTCTCTCGGTATTGTAAACggt                                                            <  1:2182992/48‑2 (MQ=37)
                              aTGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGt                                  >  1:286995/1‑71 (MQ=255)
                              aTGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGt                                  >  1:2788350/1‑71 (MQ=255)
                               tGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTg                                                 >  1:2777044/1‑55 (MQ=255)
                               tGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGtt                                 <  1:2209204/71‑1 (MQ=255)
                               tGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGtt                                 >  1:309222/1‑71 (MQ=255)
                               tGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGtt                                 <  1:1810266/71‑1 (MQ=255)
                               tGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGtt                                 <  1:1625172/71‑1 (MQ=255)
                               tGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGt                                  >  1:2818997/1‑70 (MQ=255)
                                 aaaGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACggtg                                                           <  1:823771/43‑3 (MQ=38)
                                 aaaGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTg                                >  1:2778388/1‑70 (MQ=255)
                                 aaaGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTg                                <  1:4200508/70‑1 (MQ=255)
                                 aaaGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTg                                <  1:32826/70‑1 (MQ=255)
                                 aaaGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTg                                <  1:502093/70‑1 (MQ=255)
                                  aaGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGaa                              <  1:689048/71‑1 (MQ=255)
                                   aGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGa                                                          <  1:1104164/42‑1 (MQ=37)
                                   aGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGa                               >  1:2159003/1‑69 (MQ=255)
                                     ggATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGAc                           <  1:2703001/71‑1 (MQ=255)
                                      gATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGAct                          <  1:4224753/71‑1 (MQ=255)
                                        tGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGaa                              <  1:1463095/65‑1 (MQ=255)
                                         ggTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGa                               <  1:2484374/63‑1 (MQ=255)
                                          gTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGcaa                                                     <  1:2699879/40‑3 (MQ=255)
                                          gTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTgg                                         <  1:4472966/52‑1 (MQ=255)
                                           tAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGtt                                 >  1:2286174/1‑59 (MQ=255)
                                              ccGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCa                  <  1:3503062/71‑1 (MQ=255)
                                                   gTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGAc             >  1:4060063/1‑71 (MQ=255)
                                                    tttCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGAcc            <  1:116376/71‑1 (MQ=255)
                                                     ttCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGAcc            >  1:2135402/1‑70 (MQ=255)
                                                      tCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGaa                                       <  1:1477228/42‑1 (MQ=19)
                                                      tCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGaa                                       >  1:443394/1‑42 (MQ=19)
                                                      tCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGtt                                 >  1:727257/1‑48 (MQ=25)
                                                      tCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGaa                              >  1:3504584/1‑51 (MQ=255)
                                                      tCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCg                    <  1:3258081/61‑1 (MQ=255)
                                                      tCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCg                    <  1:585111/61‑1 (MQ=255)
                                                       cTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGa          <  1:4021500/70‑1 (MQ=255)
                                                       cTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGa          <  1:2209524/70‑1 (MQ=255)
                                                         gTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCa                  >  1:78123/1‑60 (MQ=255)
                                                          tCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATg      <  1:2989136/71‑1 (MQ=255)
                                                           cGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGaa                              <  1:1820818/46‑1 (MQ=25)
                                                           cGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATg      >  1:1787282/1‑70 (MQ=255)
                                                              taTTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACg  >  1:1139677/2‑71 (MQ=255)
                                                               aTTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACg  >  1:3243315/1‑70 (MQ=255)
                                                                ttGTAAACGGTGAAGCAATTTGCGATCTGGAATAc                                    >  1:1591977/1‑35 (MQ=16)
                                                                ttGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACAt       <  1:3321201/64‑1 (MQ=255)
                                                                ttGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACg  >  1:175693/1‑69 (MQ=255)
                                                                    |                                                                
GTGGAAAACGGCAAGCTGAAAACCAATCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGAATTGTGAACGGCGAAGCGGTTTGCGATCTGGAATACGTTGAAGACTCTGCCGCAGAGACCGACATGAACG  >  W3110S.gb/3824300‑3824432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: