Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 3,824,374 C→T 100% pseudogene (507/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,824,3740CT100.0% 186.4 / NA 66pseudogene (507/687 nt)rphECK3633:JW3618:b3643; ribonuclease PH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/38);  total (28/38)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCAAGCTGAAAACCAATCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGAATTGTGAACGGCGAAGCGGTTTGCGATCTGGAATACGTTGAAGACTCTGCCGCAGAGACCGACATGAACGTAGTGATGA  >  W3110S.gb/3824310‑3824441
                                                                |                                                                   
gCAAGCTGAAAACCAACCCGATGAAAGGTATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGca                                                               <  1:834686/71‑2 (MQ=255)
gCAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCACAGTTTCTGTCGGTATTGTAAACGGTGAAGca                                                               <  1:1203430/71‑2 (MQ=255)
  aaGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGcaat                                                             <  1:140021/71‑4 (MQ=255)
     cTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGcaat                                                             <  1:1717685/68‑4 (MQ=255)
     cTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTg                                                          >  1:4091924/1‑71 (MQ=255)
         aaaCCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGAt                                                      >  1:2765275/1‑71 (MQ=255)
             cAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTgg                                                  <  1:2354774/71‑1 (MQ=255)
              aacccGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGa                                                 <  1:760545/68‑1 (MQ=255)
               acccGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGaa                                                <  1:3916880/69‑1 (MQ=255)
                 ccGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGaa                                                                  >  1:2769127/1‑51 (MQ=255)
                 ccGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGAt                                                      <  1:415317/63‑1 (MQ=255)
                    aTGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGt                                           >  1:286995/1‑71 (MQ=255)
                    aTGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGt                                           >  1:2788350/1‑71 (MQ=255)
                     tGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTg                                                          >  1:2777044/1‑55 (MQ=255)
                     tGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGtt                                          >  1:309222/1‑71 (MQ=255)
                     tGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGtt                                          <  1:1625172/71‑1 (MQ=255)
                     tGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGtt                                          <  1:1810266/71‑1 (MQ=255)
                     tGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGtt                                          <  1:2209204/71‑1 (MQ=255)
                     tGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGt                                           >  1:2818997/1‑70 (MQ=255)
                       aaaGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTg                                         <  1:32826/70‑1 (MQ=255)
                       aaaGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTg                                         <  1:502093/70‑1 (MQ=255)
                       aaaGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTg                                         >  1:2778388/1‑70 (MQ=255)
                       aaaGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTg                                         <  1:4200508/70‑1 (MQ=255)
                        aaGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGaa                                       <  1:689048/71‑1 (MQ=255)
                         aGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGa                                                                   <  1:1104164/42‑1 (MQ=37)
                         aGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGa                                        >  1:2159003/1‑69 (MQ=255)
                           ggATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGAc                                    <  1:2703001/71‑1 (MQ=255)
                            gATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGAct                                   <  1:4224753/71‑1 (MQ=255)
                              tGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGaa                                       <  1:1463095/65‑1 (MQ=255)
                               ggTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGa                                        <  1:2484374/63‑1 (MQ=255)
                                gTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGcaa                                                              <  1:2699879/40‑3 (MQ=255)
                                gTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTgg                                                  <  1:4472966/52‑1 (MQ=255)
                                 tAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGtt                                          >  1:2286174/1‑59 (MQ=255)
                                    ccGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCa                           <  1:3503062/71‑1 (MQ=255)
                                         gTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGAc                      >  1:4060063/1‑71 (MQ=255)
                                          tttCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGAcc                     <  1:116376/71‑1 (MQ=255)
                                           ttCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGAcc                     >  1:2135402/1‑70 (MQ=255)
                                            tCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGaa                                                >  1:443394/1‑42 (MQ=19)
                                            tCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGaa                                                <  1:1477228/42‑1 (MQ=19)
                                            tCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGtt                                          >  1:727257/1‑48 (MQ=25)
                                            tCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGaa                                       >  1:3504584/1‑51 (MQ=255)
                                            tCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCg                             <  1:3258081/61‑1 (MQ=255)
                                            tCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCg                             <  1:585111/61‑1 (MQ=255)
                                             cTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGa                   <  1:4021500/70‑1 (MQ=255)
                                             cTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGa                   <  1:2209524/70‑1 (MQ=255)
                                               gTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCa                           >  1:78123/1‑60 (MQ=255)
                                                tCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATg               <  1:2989136/71‑1 (MQ=255)
                                                 cGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGaa                                       <  1:1820818/46‑1 (MQ=25)
                                                 cGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATg               >  1:1787282/1‑70 (MQ=255)
                                                    taTTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACg           >  1:1139677/2‑71 (MQ=255)
                                                     aTTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACg           >  1:3243315/1‑70 (MQ=255)
                                                      ttGTAAACGGTGAAGCAATTTGCGATCTGGAATAc                                             >  1:1591977/1‑35 (MQ=16)
                                                      ttGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACAt                <  1:3321201/64‑1 (MQ=255)
                                                      ttGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACg           >  1:175693/1‑69 (MQ=255)
                                                           aaCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGCCCGACATGAACGTAGtgat    >  1:498858/1‑71 (MQ=255)
                                                           aaCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGa              <  1:2565892/61‑1 (MQ=255)
                                                           aaCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGtgat    <  1:231623/71‑1 (MQ=255)
                                                           aaCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGtgat    <  1:4065390/71‑1 (MQ=255)
                                                           aaCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGtgat    <  1:3992509/71‑1 (MQ=255)
                                                           aaCGGTGAAGCAATTTGCGATCTGGAATACGTCGAAGACTCAGCCGCAGAGACCGACATGAACGTAGtgat    <  1:3842845/71‑1 (MQ=255)
                                                            aCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCa                           >  1:4531103/1‑47 (MQ=37)
                                                            aCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGAc                  >  1:684489/1‑56 (MQ=255)
                                                            aCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGtgatg   <  1:1045025/71‑1 (MQ=255)
                                                            aCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGtgat    >  1:2686611/1‑70 (MQ=255)
                                                            aCGGTGAAGCAATTTGAGATCTGGAATACGTTGAAGACTCAGCCGc                            >  1:4247706/1‑46 (MQ=255)
                                                             cGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTTAGCCGCAGACACCGACATGAACGTAGtgatga  >  1:233203/1‑71 (MQ=255)
                                                                |                                                                   
GCAAGCTGAAAACCAATCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGAATTGTGAACGGCGAAGCGGTTTGCGATCTGGAATACGTTGAAGACTCTGCCGCAGAGACCGACATGAACGTAGTGATGA  >  W3110S.gb/3824310‑3824441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: