| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,915,241 | A→G | 12.3% | E48G (GAG→GGG) | glyQ → | glycine tRNA synthetase, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,915,241 | 0 | A | G | 12.3% | 167.5 / 13.6 | 65 | E48G (GAG→GGG) | glyQ | glycine tRNA synthetase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base A (27/30); new base G (5/3); total (32/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCATTGTTCAACCATTGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGCCCGACCGATGGTCGCTAC > W3110S.gb/3915175‑3915308 | ccATTGTTCAACCATTGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCt < 1:3067803/70‑1 (MQ=255)ccATTGTTCAACCATTGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTg < 1:539991/71‑1 (MQ=255) aTTGTTCAACCATTGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCt < 1:2949987/68‑1 (MQ=255) cAACCATTGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGCCAg < 1:4039037/70‑1 (MQ=255) cAACCATTGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAg < 1:827801/70‑1 (MQ=255) aaCCATTGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTgg > 1:4566463/1‑63 (MQ=255) aaCCATTGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGaa > 1:4212920/1‑71 (MQ=255) aCCATTGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAAc > 1:2979235/1‑71 (MQ=255) ccATTGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAAcc > 1:2463782/1‑71 (MQ=255) ccATTGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAAcc > 1:3531490/1‑71 (MQ=255) ccATTGGACATGAAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAAcc > 1:4102757/1‑71 (MQ=255) cATTGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCg < 1:2840036/71‑1 (MQ=255) ggACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGCCAGAACCGATg < 1:2016114/70‑1 (MQ=255) ggACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATgg > 1:21625/1‑71 (MQ=255) ggACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATgg > 1:3532797/1‑71 (MQ=255) ggACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATg > 1:3503423/1‑70 (MQ=255) aCATGGAAGTCGGCGCGTGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATggcg < 1:923754/71‑1 (MQ=255) cATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGCCAGAACCGATggcgg > 1:3469975/1‑71 (MQ=255) ggAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGgct > 1:3425323/1‑70 (MQ=255) aaGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTg < 1:1483888/47‑1 (MQ=255) gTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGAt < 1:160581/59‑1 (MQ=255) gcgGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGCCAGAACCGATGGCGGCTGCTTATGtt > 1:4245245/1‑69 (MQ=255) ggAAGCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATg < 1:4608193/51‑1 (MQ=255) ggAACCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGCCAg < 1:2454484/43‑1 (MQ=255) gAACCTCTCACCCAATGACCTGTCTGCGCGATCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCt > 1:3468450/1‑71 (MQ=255) gAACCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGtt < 1:1317128/65‑1 (MQ=255) aCCTCTCACCCAATGACCTTTCTGCGCGGGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCa > 1:1266343/1‑65 (MQ=255) ccTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAAc < 1:3008339/42‑1 (MQ=255) ccTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGAt > 1:1903607/1‑46 (MQ=255) tctcACCCAATGACCTGTCTGCGCGATCTGGGGCCAGa < 1:3917498/38‑1 (MQ=255) ctcACCCAATGACCTGTCTGCGCGGGCTGGGGCCAGAACCg > 1:3745343/1‑41 (MQ=255) ctcACCCAATGACCTGTCTGCGCGATCTGGGGCCAGAACCGATGGCGGCTGCTTa < 1:1056570/55‑1 (MQ=255) ctcACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCtt > 1:2116824/1‑66 (MQ=255) ctcACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCtcgt > 1:1770257/1‑71 (MQ=255) ctcACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCtcg < 1:1368518/70‑1 (MQ=255) tcACCCAATGACCTGTCTGCGCGATCTGGGGCCAGAACCGATGGCGGCTGCTTa < 1:4432742/54‑1 (MQ=255) tcACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGAt > 1:2513126/1‑42 (MQ=255) tcACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATggcgg > 1:1968851/1‑47 (MQ=255) tcACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCtcg < 1:84763/69‑1 (MQ=255) cACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCtcgtcg > 1:657681/1‑71 (MQ=255) cACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCtcgtcg > 1:3986464/1‑71 (MQ=255) aCCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCtcgtc < 1:3300468/69‑1 (MQ=255) aCCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGc > 1:3682105/1‑71 (MQ=255) cccAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGgctgct < 1:2818571/49‑1 (MQ=255) cccAATGACCTGGCTGCGCGAGCGGGGGCCAGAACCGATgg < 1:1688040/41‑1 (MQ=25) ccAATGACCTGTCTGCGCGGGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGca > 1:957578/1‑58 (MQ=255) ccAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGccc > 1:899472/1‑71 (MQ=255) ccAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGcc > 1:1215509/1‑70 (MQ=255) cAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGa > 1:1106977/1‑36 (MQ=255) cAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGa > 1:460688/1‑36 (MQ=255) cAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTa > 1:2743991/1‑49 (MQ=255) aTGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTAt < 1:3477871/48‑1 (MQ=255) aTGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGCccgac > 1:4368824/1‑71 (MQ=255) tGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATggc < 1:6238/37‑1 (MQ=255) aCCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGCccgaccg < 1:4249214/70‑1 (MQ=255) cTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGCCCGACCGATg < 1:4508480/71‑1 (MQ=255) cTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGCCCGACCGATg < 1:906411/71‑1 (MQ=255) gTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATg > 1:1740452/1‑42 (MQ=255) tCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGCCCGACCGATgg < 1:3585449/69‑1 (MQ=255) tGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGCCCGACCGATg > 1:4278549/1‑66 (MQ=255) tGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGCCCGACCGATg < 1:226996/66‑1 (MQ=255) cgcgAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGCCCGACCGATGGTCGc < 1:2935541/69‑1 (MQ=255) cgcgAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGCCCGACCGATGGTCGCt < 1:3188956/70‑1 (MQ=255) cgcgAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGCCCGACCGATGGTCGCTa < 1:2725159/71‑1 (MQ=255) gAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGCCCGACCGATGGTCGCTAc < 1:204347/69‑1 (MQ=255) aGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGCccga > 1:3539610/1‑54 (MQ=255) | CCATTGTTCAACCATTGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGCCCGACCGATGGTCGCTAC > W3110S.gb/3915175‑3915308 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |