| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,012,376 | G→A | 9.4% | A500T (GCC→ACC) | rbbA → | fused ribosome‑associated ATPases |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,012,376 | 0 | G | A | 9.4% | 230.4 / 10.7 | 74 | A500T (GCC→ACC) | rbbA | fused ribosome‑associated ATPases |
| Reads supporting (aligned to +/- strand): ref base G (28/39); new base A (5/2); total (33/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.31e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
ATCTCACTGATGCACGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCA‑TTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGCAACGAAGCCGA > W3110S.gb/4012307‑4012443 | aTCTCACTGATGCACGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAgc > 1:1100094/1‑71 (MQ=255) tcACTGATGCACGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAaccgc > 1:2044593/1‑71 (MQ=255) aCTGATGCACGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAg < 1:1514773/65‑1 (MQ=255) aCTGATGCACGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCa < 1:3865230/71‑1 (MQ=255) cTGATGCACGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAACCGCCAg > 1:2581693/1‑71 (MQ=255) tGATGCACGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGt > 1:4309698/1‑71 (MQ=255) tGATGCACGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGt > 1:2481405/1‑71 (MQ=255) tGATGCACGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGt > 1:2216268/1‑71 (MQ=255) cACGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGa < 1:4140233/71‑1 (MQ=255) aCGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAgc > 1:3034048/1‑58 (MQ=255) aCGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTgg < 1:4430216/69‑1 (MQ=255) cGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTgg > 1:3887074/1‑68 (MQ=255) gCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTgg < 1:4178913/67‑1 (MQ=255) gCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAACCGCCAGTCTGGaa > 1:3467875/1‑69 (MQ=255) cGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAgg > 1:1073578/1‑71 (MQ=255) cGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAgg < 1:488207/71‑1 (MQ=255) ggAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAgg > 1:4021303/1‑70 (MQ=255) ggAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGc < 1:92226/71‑1 (MQ=255) gAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTgg < 1:1959841/63‑1 (MQ=255) gAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCa > 1:4088991/1‑71 (MQ=255) aaaaGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAagag < 1:2385783/67‑1 (MQ=255) aaGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAgg < 1:1460204/66‑1 (MQ=255) aaGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCA‑ttt < 1:4528557/71‑1 (MQ=255) aaGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCA‑tt > 1:156090/1‑70 (MQ=255) aGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCA‑TTTa > 1:1700671/1‑71 (MQ=255) gCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCt > 1:2575656/1‑54 (MQ=255) cTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAaga > 1:2282359/1‑59 (MQ=255) tGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCA‑TTTATCGcc > 1:3475786/1‑70 (MQ=255) tGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCA‑TTTATCGcc > 1:1043421/1‑70 (MQ=255) gCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCA‑TTTATCGcc < 1:3643597/69‑1 (MQ=255) gCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCA‑TTTATCGCCTa > 1:2372235/1‑71 (MQ=255) gCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAACCGCCAGTCTGGAAGAGGCA‑TTTATCGCCTa > 1:4371731/1‑71 (MQ=255) cAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCA‑TTTATCGCCTAtt < 1:4221126/71‑1 (MQ=255) gCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCA‑TTTATCGCCTAtt > 1:1444732/1‑69 (MQ=255) gCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCA‑TTTATCGCCTATTTg < 1:3885545/71‑1 (MQ=255) gCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCA‑TTTATCGCCTATTTg < 1:2978109/71‑1 (MQ=255) gCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAACCGCCAGTCTGGAAGAGGCA‑ttt < 1:4536850/59‑1 (MQ=255) cGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCA‑TTTATCGCCTATTTg > 1:2680751/1‑70 (MQ=255) aggTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCA‑TTTATCGCCTATTTg > 1:1191591/2‑70 (MQ=255) acacCGCAGGAACTGGTTGAGAAACGCGGAACCGCCAGTCTGGAAGAGGCA‑TTTATCGCCTATTTGCAgg > 1:333886/1‑70 (MQ=255) cacCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCA‑TTTATCGCCTATTTGCAg < 1:3256425/68‑1 (MQ=255) acCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCA‑TTTATCGCCTATTTGCAGGAAg > 1:3260163/1‑71 (MQ=255) ccGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGaa < 1:268521/42‑1 (MQ=255) cGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCA‑t < 1:2933804/48‑1 (MQ=255) cAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCA‑TTTATCGCCTATTTGCAGGAAGCgg > 1:2158961/1‑70 (MQ=255) gAACTGGTTGAGAAACGCGGAACCGCCAGTCTGGAAGAGGCA‑TTTATc > 1:4416762/1‑48 (MQ=255) aaCTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCA‑TTTATCGCCTATTTGCAGGAAGCggcaggg > 1:3121323/1‑71 (MQ=255) aCTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAaga > 1:3124612/1‑36 (MQ=255) aCTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCA‑TTTATCGCCTATTTGCAGGAAGc > 1:3953749/1‑63 (MQ=255) aCTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCA‑TTTATCGCCTATTTGCAGGAAGCggcaggg > 1:2688326/1‑70 (MQ=255) aCTGGTTGAGAAACGCGGAGCCGCCAGTCTCGAAGAGGCA‑TTTATCGCCTATTTGCAGGAAGCggcagggc < 1:4215182/71‑1 (MQ=255) cTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTTATCGCCTATTTGCAGGAAGCggcaggg > 1:293597/1‑70 (MQ=255) cTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCA‑TTTATCGCCTATTTGCAGGAAGCggcagggca < 1:2717548/71‑1 (MQ=255) cTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCA‑TTTATCGCCTATTTGCAGGAAGCggcagggca < 1:1654888/71‑1 (MQ=255) cTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCA‑TTTATCGCCTATTTGCAGGAAGCggcagggca < 1:2444069/71‑1 (MQ=255) cTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCA‑TTTATCGCCTATTTGCAGGAAGCggcagggca < 1:3333810/71‑1 (MQ=255) ggTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCA‑TTTATCGCCTATTTGCAGGAAGCggcagggca > 1:2895825/1‑69 (MQ=255) ggTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCA‑TTTATCGCCTATTTGCAGGAAGCggcagggca > 1:602267/1‑69 (MQ=255) gTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCA‑TTTATCGCCTATTTGCAGGAAGCggcagggcag < 1:1798560/69‑1 (MQ=255) gTTGAGAAACGCGGAACCGCCAGTCTGGAAGAGGCA‑TTTATCGCCTATTTGCAGGAAGCggcagggcag < 1:743188/69‑1 (MQ=255) ttGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCA‑TTTATCGCCTATTTg < 1:345205/50‑1 (MQ=255) ttGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCA‑TTTATCGCCTATTTGCAGGAAGCggcaggg < 1:4159733/65‑1 (MQ=255) ttGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCA‑TTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGc < 1:1874177/71‑1 (MQ=255) tGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCA‑TTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGc > 1:3004803/1‑70 (MQ=255) gagaAACGCGGAGCCGCCAGTCTGGAAGAGGCA‑TTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGCa < 1:4051510/70‑1 (MQ=255) gagaAACGCGGAGCCGCCAGTCTGGAAGAGGCA‑TTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGCa < 1:1832528/70‑1 (MQ=255) agaAACGCGGAGCCGCCAGTCTGGAAGAGGCA‑ttt < 1:622479/35‑1 (MQ=255) gaAACGCGGAGCCGCCAGTCTGGAAGAGGCA‑TTTATCGCCt < 1:3145767/41‑1 (MQ=255) gaAACGCGGAGCCGCCAGTCTGGAAGAGGCA‑TTTATCGCCTATTTGCAGGAAGCggcaggg < 1:1663728/61‑1 (MQ=255) gaAACGCGGAGCCGCCAGTCTGGAAGAGGCA‑TTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGCAAcg < 1:2753011/71‑1 (MQ=255) gaAACGCGGAGCCGCCAGTCTGGAAGAGGCA‑TTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGCAAcg < 1:4184919/71‑1 (MQ=255) aaCGCGGAGCCGCCAGTCTGGAAGAGGCA‑TTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGCAAcgaa < 1:3050729/71‑1 (MQ=255) aaCGCGGAGCCGCCAGTCTGGAAGAGGCA‑TTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGCAAc > 1:3985870/1‑68 (MQ=255) gcgGAGCCGCCAGTCTGGAAGAGGCA‑TTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGCAAcgaagcc < 1:3019027/71‑1 (MQ=255) ggAGCCGCCAGTCTGGAAGAGGCA‑TTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGCAAcgaagccga < 1:943319/71‑1 (MQ=255) ggAGCCGCCAGTCTGGAAGAGGCA‑TTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGCAAcgaagccga < 1:1410362/71‑1 (MQ=255) ggAGCCGCCAGTCTGGAAGAGGCA‑TTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGCAAcgaagccga < 1:4021655/71‑1 (MQ=255) ggAGCCGCCAGTCTGGAAGAGGCA‑TTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGCAAcgaagccga < 1:2147559/71‑1 (MQ=255) gccgccAGTCTGGAAGAGGCA‑TTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGCAAcgaagccga > 1:1906260/1‑68 (MQ=255) | ATCTCACTGATGCACGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCA‑TTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGCAACGAAGCCGA > W3110S.gb/4012307‑4012443 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |