| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 241,172 | C→T | 15.4% | G711D (GGT→GAT) | fadE ← | acyl coenzyme A dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 241,172 | 0 | C | T | 15.4% | 134.8 / 15.4 | 52 | G711D (GGT→GAT) | fadE | acyl coenzyme A dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (25/19); new base T (6/2); total (31/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.49e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01 | |||||||||||
GCAATCACATCCACCAGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTC > W3110S.gb/241103‑241242 | gcAATCACATCCACCAGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGAcc > 1:1275095/1‑71 (MQ=255) aaTCACATCCACCAGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGATcgc < 1:4341535/71‑1 (MQ=255) aaTCACATCCACCAGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACcgc < 1:403139/71‑1 (MQ=255) tCACATCCACCAGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGa > 1:2183975/1‑71 (MQ=255) acaTCCACCAGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGAcc < 1:1022474/71‑1 (MQ=255) aTCCACCAGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGAcc < 1:4198598/63‑1 (MQ=255) tCCACCAGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAAt < 1:3036567/71‑1 (MQ=255) tCCACCAGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAAt > 1:2961293/1‑71 (MQ=255) ccaccaGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGAcc > 1:260375/1‑61 (MQ=255) ccaccaGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATg > 1:2103973/1‑71 (MQ=255) caccaGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACGAATGc < 1:1691889/71‑1 (MQ=255) ccaGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCTATGCgg < 1:601000/71‑1 (MQ=255) caGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGAcc > 1:2222463/1‑57 (MQ=255) caGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCgg > 1:4002690/1‑70 (MQ=255) aGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCaa > 1:2821696/1‑64 (MQ=255) gcgcCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGATCGCGACCAATGCGGGaa > 1:3878250/1‑71 (MQ=255) ctctTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACggg < 1:760761/71‑1 (MQ=255) ctctTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACggg < 1:2252072/71‑1 (MQ=255) tctTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGaa > 1:3747475/1‑66 (MQ=255) ttCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGt < 1:580434/69‑1 (MQ=255) ttCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTgg > 1:4161398/1‑71 (MQ=255) ttCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTgg > 1:3534928/1‑71 (MQ=255) ccAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAAc > 1:1131906/1‑63 (MQ=255) gCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGATCGCGACCaa > 1:1140078/1‑51 (MQ=255) gCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTc > 1:2212768/1‑71 (MQ=255) gCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTc > 1:1910955/1‑71 (MQ=255) gCAAGCCAACCGGATTATGCTCGCGCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGtt < 1:2508483/70‑1 (MQ=255) cAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCg > 1:2549212/1‑71 (MQ=255) aaGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAAt > 1:1211153/1‑50 (MQ=255) aGCGAACCGGATTATGCTCGCTCGGGGTCAGGTACTGACCGCGACCAATGCGGGaa > 1:1308094/1‑56 (MQ=255) gCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCa > 1:853995/1‑71 (MQ=255) ccAACCGGATTATGCTCGCTCGGCGTCAGGTACTGAcc > 1:1611396/1‑38 (MQ=255) aaCCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACtt < 1:2624839/71‑1 (MQ=255) aaCCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACtt < 1:2630377/71‑1 (MQ=255) aCCGGATTATGCTCGCTCGGCGTCAGGTACTGATCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTg > 1:1637039/1‑71 (MQ=255) aCCGGATTATGCTCGCTCGGCGTCAGGTACTGATCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTg > 1:1642476/1‑71 (MQ=255) aCCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTg > 1:9117/1‑71 (MQ=255) ccGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTg > 1:1582464/1‑70 (MQ=255) cGGATTATGCTCGCTCGGCGTCAGGTACTGATCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTa < 1:3836192/71‑1 (MQ=255) gATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTaa > 1:1971847/1‑70 (MQ=255) tATGCTCGCTCGGCGTCAGGTACTGATCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATc > 1:4458834/1‑71 (MQ=255) tATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATc > 1:1293496/1‑71 (MQ=255) ttctcgctcgGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCtt < 1:87493/69‑1 (MQ=255) ctcgctcgGCGTCAGGTACTGACCGCGACCAATGCGGGAACgg > 1:3949595/1‑43 (MQ=255) ctcgctcgGCGTCAGGTACTGACCGCGACCAATGCGGGAACg < 1:109438/42‑1 (MQ=255) tcgctcgGCGTCAGGTACTGATCGCGACCAATGCGGGAACGGGt > 1:248527/1‑44 (MQ=255) cgGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTaaa < 1:3983107/58‑1 (MQ=255) ggCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACtt > 1:400376/1‑70 (MQ=255) gCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTa < 1:798817/54‑1 (MQ=255) tCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATg < 1:2752804/70‑1 (MQ=255) tCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGa > 1:177602/1‑71 (MQ=255) ggTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATcc < 1:1719165/71‑1 (MQ=255) gTACTGACCGCGACCAATGCGGGAACGGGTGGCGt < 1:963341/35‑1 (MQ=255) aCTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACtt > 1:2346220/1‑42 (MQ=255) tGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATCCAGCtt > 1:81313/1‑71 (MQ=255) gACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATCCAGCTTg > 1:4449904/1‑71 (MQ=255) aCCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCt < 1:2916210/47‑1 (MQ=255) ccGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGt > 1:3835641/1‑70 (MQ=255) ccGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTc < 1:4331572/71‑1 (MQ=255) | GCAATCACATCCACCAGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTC > W3110S.gb/241103‑241242 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |