| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,093,241 | C→A | 8.6% | G207C (GGT→TGT) | gntT ← | gluconate transporter, high‑affinity GNT I system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,093,241 | 0 | C | A | 8.6% | 224.3 / 4.8 | 69 | G207C (GGT→TGT) | gntT | gluconate transporter, high‑affinity GNT I system |
| Reads supporting (aligned to +/- strand): ref base C (33/30); new base A (2/4); total (35/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.28e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.00e-01 | |||||||||||
AGAGGTCCAGACGCTGACGCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAACCGGACCGGCGAGAATCACGGTCGG > W3110S.gb/4093173‑4093310 | agagGTCCAGACGCTGACGCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCtt < 1:2069556/71‑1 (MQ=255) gTCCAGACGCTGACGCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCt < 1:2521722/66‑1 (MQ=255) cAGACGCTGACGCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAAt > 1:161298/1‑71 (MQ=255) cAGACGCTGACGCCAAAGCTCGGCATCTCTTCTTCGCTG‑AGGTTTTCGCGCTGTAGAGACATTCCGGAAtt < 1:2684691/71‑1 (MQ=255) aGACGCTGACGCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAAtt < 1:2391335/71‑1 (MQ=255) aGACGCTGACGCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACATTCCGGAAtt < 1:4204637/71‑1 (MQ=255) gACGCTGACGCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTg > 1:3255493/1‑71 (MQ=255) aCGCTGACGCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTgg > 1:3439028/1‑71 (MQ=255) gCTGACGCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGaa > 1:977623/1‑65 (MQ=255) gCTGACGCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCt < 1:650564/71‑1 (MQ=255) gCTGACGCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCt < 1:1845803/71‑1 (MQ=255) tGACGCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAAtt > 1:3376058/1‑65 (MQ=255) aCGCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTa < 1:1732867/69‑1 (MQ=255) cGCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATc > 1:4291340/1‑70 (MQ=255) gCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGa > 1:4609495/1‑71 (MQ=255) ccAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGa > 1:1250959/1‑70 (MQ=255) cAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATa > 1:2927984/1‑71 (MQ=255) cAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATa > 1:1261844/1‑71 (MQ=255) cAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATa > 1:1408459/1‑71 (MQ=255) aaaGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATAc > 1:3414112/1‑71 (MQ=255) aaGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCTCTGTAGAGACCTTACGGAATTGGCTTATCg > 1:4470787/1‑66 (MQ=255) aaGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATAcc > 1:976529/1‑71 (MQ=255) aaGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATAcc < 1:4483552/71‑1 (MQ=255) aGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCt > 1:2243704/1‑71 (MQ=255) aGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCt > 1:190639/1‑71 (MQ=255) gCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTcc < 1:2834792/49‑1 (MQ=255) gCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAac < 1:2794584/71‑1 (MQ=255) gCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACATTCCGGAATTGGCTTATCGATACCTTTCaa > 1:4159512/1‑70 (MQ=255) cATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAaca > 1:1200231/1‑71 (MQ=255) aTCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAacac > 1:1002755/1‑71 (MQ=255) tctcTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAAtt < 1:1659902/47‑1 (MQ=255) tcttctTCGCTGAAGGTTTTCGCGCTGTAGAGACATTCCGGAATTGGCTTATCGATACCTTTCAACAcgc > 1:3008377/1‑70 (MQ=255) ttcttcGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGaa < 1:77249/41‑1 (MQ=255) ttcttcGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATc < 1:3658003/51‑1 (MQ=255) ttcttcGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAacac > 1:2183459/1‑66 (MQ=255) ttcttcGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACAcgc < 1:1988532/68‑1 (MQ=255) ttcatcGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAg > 1:1623254/1‑71 (MQ=255) tcttcGCTGAAGGTTTTCGCGCTGTAGAGACCTTGCGGAATTGGCTTATCGATACCTTTCAACACGCGAGc > 1:4157900/1‑71 (MQ=255) tcttcGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCgg < 1:1811896/38‑1 (MQ=255) cttcGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGa < 1:366000/68‑1 (MQ=255) cttcGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCg > 1:2564704/1‑71 (MQ=255) tcGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTc > 1:3606111/1‑57 (MQ=255) cGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGt < 1:718226/69‑1 (MQ=255) gCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTaaa < 1:71254/71‑1 (MQ=255) gCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTaaa < 1:76735/71‑1 (MQ=255) gCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTaa < 1:951521/70‑1 (MQ=255) tGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTc < 1:2018292/53‑1 (MQ=255) gAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAaccg > 1:14302/1‑71 (MQ=255) aaGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGa > 1:3524823/1‑43 (MQ=255) ggTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAaccggac > 1:2431388/1‑71 (MQ=255) ggTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAaccgga > 1:496670/1‑70 (MQ=255) gTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAaccggacc < 1:2746481/71‑1 (MQ=255) ttttCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAaccggacc < 1:3658524/70‑1 (MQ=255) ttCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAaccggaccgg > 1:1151735/1‑70 (MQ=255) tCGCGCTGTAGAGACATTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAACCGGACCGGc < 1:4443956/70‑1 (MQ=255) gcgcTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTaa < 1:2676189/57‑1 (MQ=255) cTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAaccgg > 1:3142748/1‑59 (MQ=255) cTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAACCGGACCGGCGAGAAt < 1:3061771/71‑1 (MQ=255) tAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAACCGGACCGGCGAGAAt > 1:4494890/1‑68 (MQ=255) tAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAACCGGACCGGCGAGAATCAc < 1:4345893/71‑1 (MQ=255) gagaCCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAACCGGACCGGCga < 1:4525121/62‑1 (MQ=255) gagaCCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAACCGGACCGGCGAGAATCACgg > 1:431669/1‑71 (MQ=255) gagaCCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAACCGGACCGGCGAGAATCACg < 1:3581929/70‑1 (MQ=255) gaCCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAacc > 1:2210381/1‑50 (MQ=255) gaCCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAACCGGACCGGCGAGAATCACGGTc < 1:3615053/71‑1 (MQ=255) gaCCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAACCGGACCGGCGAGAATCACGGTc > 1:196790/1‑71 (MQ=255) gaCATTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAACCGGACCGGCGAGAATCACGGTc < 1:1453850/71‑1 (MQ=255) aCCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTaa < 1:4353998/46‑1 (MQ=255) aCCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAaccggaccgg < 1:381149/56‑1 (MQ=255) cTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAACCGGACCGGCg > 1:1115708/1‑56 (MQ=255) cTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAACCGGACCGGCGAGAATCACGGTCgg > 1:2846543/1‑70 (MQ=255) cTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAACCGGACCGGCGAGAATCACGGTCgg > 1:2124182/1‑70 (MQ=255) | AGAGGTCCAGACGCTGACGCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAACCGGACCGGCGAGAATCACGGTCGG > W3110S.gb/4093173‑4093310 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |