| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 267,502 | G→T | 12.4% | P228H (CCT→CAT) | ykfA ← | predicted GTP‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 267,502 | 0 | G | T | 12.4% | 223.7 / 10.2 | 72 | P228H (CCT→CAT) | ykfA | predicted GTP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base G (24/38); new base T (4/5); total (29/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
GAGTACGGCCCCGACGGTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTAATCATCCGCTCGGCCATCAC > W3110S.gb/267432‑267572 | gAGTACGGCCCCGACGGTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAaaggaag > 1:4504498/1‑71 (MQ=255)gAGTACGGCCCCGACGGTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAaaggaag < 1:3230950/71‑1 (MQ=255) aGTACGGCCCCGACGGTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAaaggaagg < 1:1741564/71‑1 (MQ=255) tACGGCCCCGACGGTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAATGAt < 1:4371674/71‑1 (MQ=255) cGGCCCCGACGGTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATgg < 1:727933/71‑1 (MQ=255) ggCCCCGACGGTTTCACCAAAATCGCTACGAGCCAGTTCCCGGACTACCGTCGTGCGAAAGGAatgatg > 1:3296758/1‑69 (MQ=255) gCCCCGACGGTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAg < 1:3405861/71‑1 (MQ=255) cccGACGGTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGtt < 1:3806413/71‑1 (MQ=255) cccGACGGTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGtt < 1:772842/71‑1 (MQ=255) cccGACGGTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGtt < 1:284716/71‑1 (MQ=255) ccGACGGTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGtt < 1:252281/70‑1 (MQ=255) cGACGGTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGc > 1:2441432/1‑71 (MQ=255) cGACGGTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGc < 1:536189/71‑1 (MQ=255) gACGGTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGc > 1:1637414/1‑70 (MQ=255) aCGGTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGa > 1:4373833/1‑71 (MQ=255) aCGGTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGa > 1:1597666/1‑71 (MQ=255) cGGTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTg > 1:362370/1‑67 (MQ=255) tttCACCAAAATCTCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGacac > 1:1763707/1‑70 (MQ=255) tttCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGacac > 1:1202850/1‑70 (MQ=255) tCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGAcaccac > 1:777257/1‑71 (MQ=255) tCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGAcaccac > 1:1315008/1‑71 (MQ=255) tCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAACGATg > 1:4050276/1‑56 (MQ=255) aCCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGacac < 1:629174/66‑1 (MQ=255) aCCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGAcacca < 1:3448190/68‑1 (MQ=255) aCCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACaccacc > 1:3087983/1‑70 (MQ=255) aCCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCg < 1:4177810/71‑1 (MQ=255) ccAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATg < 1:3802589/53‑1 (MQ=255) cAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCggg > 1:57445/1‑71 (MQ=255) cAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCgg > 1:2177816/1‑70 (MQ=255) cAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCgg > 1:3012892/1‑70 (MQ=255) cAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCAACggg > 1:1688052/1‑71 (MQ=255) aaaTCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAATGATGGAGTTGCGACACCACCGGGCt > 1:1250712/1‑71 (MQ=255) aaTCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCtg < 1:4462824/71‑1 (MQ=255) aTCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAGAGGAAGGATGGAGTTGCGACACCACCGGGCtgg > 1:4079141/1‑71 (MQ=255) tCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCtggt < 1:604730/71‑1 (MQ=255) tCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCtggt < 1:3119421/71‑1 (MQ=255) gCTACGAGCCTTTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCtggtgg < 1:1118696/71‑1 (MQ=255) tACGAGCCTGTTCCCGGACTACCGTCGTGCGAAaggaagga > 1:928767/1‑41 (MQ=255) tACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGt > 1:2714390/1‑47 (MQ=255) tACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGt > 1:3914524/1‑47 (MQ=255) aCGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCt > 1:1451723/1‑71 (MQ=255) cGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAATGATGGAGTTGCGACACCACCGGGCtggt > 1:2512992/1‑65 (MQ=255) cGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCt > 1:4128932/1‑70 (MQ=255) cGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCt > 1:4246320/1‑70 (MQ=255) cGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCt < 1:20320/70‑1 (MQ=255) gAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACaccacc < 1:3058817/56‑1 (MQ=255) gCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACg > 1:4507633/1‑71 (MQ=255) ccTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGc < 1:1607888/64‑1 (MQ=255) ccTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGt > 1:1381316/1‑71 (MQ=255) cTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTg < 1:510299/71‑1 (MQ=255) gTTCCCGGACTACCGTCGTGCGAAAGGAATGATGGAGtt < 1:1713897/39‑1 (MQ=255) gTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCg > 1:1149417/1‑42 (MQ=255) ttCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTggc < 1:3973818/70‑1 (MQ=255) ttCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTgg < 1:2255104/69‑1 (MQ=255) ttCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTgg < 1:691478/69‑1 (MQ=255) cccGGACTACCGTCGTGCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTggcag < 1:3353535/70‑1 (MQ=255) cccGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCtggtg > 1:2838516/1‑56 (MQ=255) cGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTggcaggca < 1:1308812/71‑1 (MQ=255) gACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTggc < 1:2015575/64‑1 (MQ=255) gACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCAc > 1:3632771/1‑70 (MQ=255) aCTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACtt < 1:3777657/71‑1 (MQ=255) cTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTa < 1:2037990/71‑1 (MQ=255) tACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCtgg < 1:4430126/47‑1 (MQ=255) aCCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTggca < 1:231681/61‑1 (MQ=255) ccGTCGTGCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTaa > 1:56073/1‑69 (MQ=255) ccGTCGTGCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTaa < 1:4372228/69‑1 (MQ=255) ccGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTgg < 1:4444906/58‑1 (MQ=255) ccGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTaa < 1:3381265/69‑1 (MQ=255) cgtcgtGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTAatca < 1:665051/71‑1 (MQ=255) cgtcgtGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTAatca < 1:4424954/71‑1 (MQ=255) gtcgtGCGAAAGGAAGGATGGAGTTGCGACACCACCgg < 1:3113211/38‑1 (MQ=255) tcgtGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTggcaggc < 1:2817057/60‑1 (MQ=255) gtGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTAATCATCCg < 1:344593/71‑1 (MQ=255) cGAAAGGAATGATGGAGTTGCGACACCACCGGGCtg < 1:4535219/36‑1 (MQ=255) gAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTAat < 1:2414326/61‑1 (MQ=255) gAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTAATCATCCGCTCg < 1:3781760/71‑1 (MQ=255) aaaGGAAGGATGGAGTTGCGACACCACCGGGCtggt < 1:4121698/36‑1 (MQ=255) aggaatgaTGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTAATCATCCGCTCGGc > 1:2995573/1‑70 (MQ=255) aggaaggaTGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTg > 1:3204133/1‑45 (MQ=255) aggaaggaTGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTAATCATCCGCTCGGcc < 1:3474773/71‑1 (MQ=255) aggaaggaTGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTAATCATCCGCTCGGcc < 1:1654165/71‑1 (MQ=255) aggaaggaTGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTAATCATCCGCTCGGc > 1:383588/1‑70 (MQ=255) aggaaggaTGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTAATCATCCGCTCGGc > 1:3795748/1‑70 (MQ=255) gaatgaTGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTg < 1:3999684/43‑1 (MQ=255) ggaTGGAGTTGCGACACCACCGGGCTGGTGGCCTCAc < 1:1082633/37‑1 (MQ=255) ggaTGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTAATCATCCGCTCGGCCATCAc < 1:946531/71‑1 (MQ=255) | GAGTACGGCCCCGACGGTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTAATCATCCGCTCGGCCATCAC > W3110S.gb/267432‑267572 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |