| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,244,991 | T→A | 5.9% | I286F (ATC→TTC) | xylE ← | D‑xylose transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,244,991 | 0 | T | A | 5.9% | 184.7 / 2.5 | 67 | I286F (ATC→TTC) | xylE | D‑xylose transporter |
| Reads supporting (aligned to +/- strand): ref base T (33/30); new base A (2/2); total (35/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCC > W3110S.gb/4244922‑4245060 | cccccAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATg < 1:1638606/71‑1 (MQ=255)cccccAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATg < 1:622427/71‑1 (MQ=255) ccAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGgag < 1:2232543/71‑1 (MQ=255) aGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGgag > 1:4214928/1‑69 (MQ=255) gTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGagag < 1:1373561/68‑1 (MQ=255) gTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCAt < 1:2314162/71‑1 (MQ=255) tGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTAc < 1:3215100/70‑1 (MQ=255) gAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGAAGGAGAGCATTa < 1:2122908/68‑1 (MQ=255) acacTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATTGAGAGCATTACGCCg < 1:2314116/71‑1 (MQ=255) acTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGAt < 1:87901/71‑1 (MQ=255) acTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGAt < 1:2080991/71‑1 (MQ=255) ttCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTa > 1:3743619/1‑71 (MQ=255) ttCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTa > 1:40694/1‑71 (MQ=255) ccGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTa < 1:3884453/69‑1 (MQ=255) gcgTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATcac > 1:847147/1‑71 (MQ=255) gtagtaCAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATca > 1:905434/1‑68 (MQ=255) tagtaCAGCACCACATTGATGCCGACAAATTGCTGGAAGATg < 1:543405/42‑1 (MQ=255) agtaCAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATcacgcc > 1:660729/1‑70 (MQ=255) agtaCAGCACCACATTGATGCCGACAAATTGCTGGAAGAAGGagag > 1:1780264/1‑46 (MQ=255) agtaCAGCACCACATTGATGCCGACAAATTGCTGGAAGAAGGAGAGc > 1:434606/1‑47 (MQ=255) taCAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCAt < 1:3389001/47‑1 (MQ=255) aCAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTAc > 1:4332926/1‑49 (MQ=255) cAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCa > 1:2423856/1‑44 (MQ=255) cAGCACCACATTGATGCCGACAAATAGCTGGAAGATGga > 1:1596692/1‑39 (MQ=255) aGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACa > 1:898243/1‑57 (MQ=255) caccacATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATcac > 1:4227074/1‑60 (MQ=255) acctcATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATca < 1:3022060/58‑1 (MQ=255) accacATTGATGCCGACAAATTGCTGGAAGATGGAGAGCAt > 1:3178293/1‑41 (MQ=255) ccacATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCaaa > 1:658831/1‑70 (MQ=255) acaTTGATGCCGACAAATTGCTGGAAGATGGAGAGCAt < 1:86876/38‑1 (MQ=255) acaTTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACAt > 1:2679518/1‑71 (MQ=255) acaTTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACAt < 1:1112963/71‑1 (MQ=255) acaTTGATGCCGACAAATTGCTGGAAGAAGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACAt < 1:2651402/71‑1 (MQ=255) caTTGATGCCGACAAATTGCTGGAAGATGGAGAGCa > 1:484201/1‑36 (MQ=255) caTTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATcacgccc < 1:2276497/59‑1 (MQ=255) caTTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATc < 1:2270668/71‑1 (MQ=255) caTTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATc < 1:1768968/71‑1 (MQ=255) aTTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTAc < 1:1984876/48‑1 (MQ=255) ttGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTAc < 1:1893014/47‑1 (MQ=255) gATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATc > 1:3916881/1‑49 (MQ=255) gATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATcag > 1:493924/1‑69 (MQ=255) gATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATca > 1:471492/1‑68 (MQ=255) aTGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATcagca < 1:1157461/70‑1 (MQ=255) tGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAga < 1:3907229/71‑1 (MQ=255) cGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAgacga > 1:212565/1‑71 (MQ=255) gACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAAt < 1:1199617/42‑1 (MQ=255) aCAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATc < 1:116104/60‑1 (MQ=255) cAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATcacg > 1:849827/1‑44 (MQ=255) cAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATc > 1:3679494/1‑41 (MQ=255) cAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCaaa > 1:3589897/1‑55 (MQ=255) aaaTTGCTGGAAGATGGAGAGCATTACGCCGATTACa > 1:1728674/1‑37 (MQ=255) aaTTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATcag < 1:3015180/59‑1 (MQ=255) aTTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGaccacc < 1:2987874/70‑1 (MQ=255) aTTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCg > 1:1648836/1‑71 (MQ=255) ttGCTGGAAGATGGAGAGCATTACGCCGATTACAAt < 1:642407/36‑1 (MQ=255) tGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACAt > 1:71857/1‑53 (MQ=255) gCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGtt > 1:1415999/1‑71 (MQ=255) gCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGt > 1:29898/1‑70 (MQ=255) cTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGttt < 1:497952/71‑1 (MQ=255) cTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGttt < 1:1505935/71‑1 (MQ=255) gAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAAc > 1:2362503/1‑46 (MQ=255) aaGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATca > 1:3630070/1‑49 (MQ=255) aaGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCg > 1:740244/1‑71 (MQ=255) aGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCgg > 1:1737970/1‑71 (MQ=255) aGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCgg > 1:4256382/1‑71 (MQ=255) aTGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGcc < 1:3224232/71‑1 (MQ=255) aTGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGc > 1:2177947/1‑70 (MQ=255) | CCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCC > W3110S.gb/4244922‑4245060 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |