| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,315,024 | C→T | 10.3% | D213N (GAC→AAC) | alsA ← | fused D‑allose transporter subunits and ATP‑binding components of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,315,024 | 0 | C | T | 10.3% | 205.8 / 11.3 | 68 | D213N (GAC→AAC) | alsA | fused D‑allose transporter subunits and ATP‑binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base C (31/30); new base T (5/2); total (36/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.34e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.48e-01 | |||||||||||
GTCATTTGACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGA > W3110S.gb/4314956‑4315094 | gTCATTTGACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCg > 1:569776/1‑70 (MQ=255)gTCATTTGACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCg > 1:430579/1‑70 (MQ=255) tCATTTGACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCg > 1:4467620/1‑69 (MQ=255) tCATTTGACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGc > 1:1768145/1‑70 (MQ=255) cATTTGACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCa > 1:210407/1‑70 (MQ=255) aTTTGACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTTGCaaa > 1:822380/1‑71 (MQ=255) aTTTGACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCaaa < 1:3167123/71‑1 (MQ=255) tttGACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACAGTATAGCGGTCGCAAAt < 1:4100456/71‑1 (MQ=255) ttGACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATa > 1:4358420/1‑71 (MQ=255) tGACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATa > 1:834740/1‑70 (MQ=255) tGACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATAc > 1:3746840/1‑71 (MQ=255) gACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATAc < 1:1556811/70‑1 (MQ=255) acaTCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGc > 1:194216/1‑70 (MQ=255) acaTCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCg < 1:4494577/71‑1 (MQ=255) acaTCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCg < 1:266859/71‑1 (MQ=255) aTCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACg < 1:2010450/66‑1 (MQ=255) cGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTTGCAAATACGGCGaa > 1:4496880/1‑69 (MQ=255) cGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGaa > 1:3087837/1‑69 (MQ=255) gCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTTGCAAATa > 1:3338527/1‑61 (MQ=255) gCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTTGCAAATACGGCGAAtt < 1:196706/70‑1 (MQ=255) gCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGTCGAAttt < 1:1002750/71‑1 (MQ=255) gCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAAttt < 1:2478883/71‑1 (MQ=255) cTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTc > 1:2600195/1‑71 (MQ=255) ttACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGaa < 1:2666667/66‑1 (MQ=255) tACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTc > 1:1910089/1‑69 (MQ=255) aCTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGc > 1:2988395/1‑71 (MQ=255) tATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATAc < 1:3108172/56‑1 (MQ=255) tGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAAc > 1:3676559/1‑71 (MQ=255) ccGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACt > 1:504469/1‑70 (MQ=255) ccGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACt > 1:3375991/1‑70 (MQ=255) cGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTa < 1:2628851/71‑1 (MQ=255) gCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTAt < 1:219076/71‑1 (MQ=255) gCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTAt < 1:1671910/71‑1 (MQ=255) cTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCa > 1:1141771/1‑44 (MQ=255) gCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTTGCAAATACGGCGAATTTCCGCCAACTTATGc > 1:4021119/1‑70 (MQ=255) aaaCGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGc > 1:3784089/1‑68 (MQ=255) aCGCTGCTGCCGTCTTTCATCACCGTATAGCGGTTGCAAATACGGCGAATTTCCGCCAACTTATGCGAGat < 1:1448146/71‑1 (MQ=255) cGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGata > 1:1299157/1‑71 (MQ=255) ctgctgCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGAtata < 1:3051968/71‑1 (MQ=255) gctgcCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCgag > 1:3862612/1‑64 (MQ=255) gctgcCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGa > 1:2601224/1‑71 (MQ=255) ctgcCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGAc > 1:3954023/1‑71 (MQ=255) ctgcCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGAc < 1:3761130/71‑1 (MQ=255) ccGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGata < 1:884723/63‑1 (MQ=255) ccGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGAt < 1:3195030/71‑1 (MQ=255) ccGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGAt > 1:683646/1‑71 (MQ=255) ccGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGAt < 1:3466485/71‑1 (MQ=255) cGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGAt < 1:1385600/70‑1 (MQ=255) gTCTTTCATCACCGTATAGCGGTTGCAAATACGGCGAATTTCCGCCAACTTATGc > 1:2722837/1‑55 (MQ=255) tttCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATg > 1:100746/1‑51 (MQ=255) tcatcaCCGTATAGCGGTCGCAAATACGGCGAATTTcc > 1:2726068/1‑38 (MQ=255) tcatcaCCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCg < 1:3311451/69‑1 (MQ=255) tcatcaCCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGt < 1:1801731/70‑1 (MQ=255) tcatcaCCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTa > 1:3599708/1‑71 (MQ=255) tcatcaCCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTa > 1:333835/1‑71 (MQ=255) catcaCCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTAt < 1:5781/47‑1 (MQ=255) catcaCCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGc < 1:1571642/49‑1 (MQ=255) catcaCCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTa < 1:2876631/70‑1 (MQ=255) atcaCCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTa > 1:2224682/1‑69 (MQ=255) caCCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGc < 1:2578226/46‑1 (MQ=255) aCCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCctc < 1:3877420/71‑1 (MQ=255) ccGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCctct > 1:2585588/1‑71 (MQ=255) gTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGAt > 1:4123922/1‑56 (MQ=255) taGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACg > 1:24093/1‑71 (MQ=255) gCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGc < 1:211013/70‑1 (MQ=255) gTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACt < 1:1693072/71‑1 (MQ=255) gTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACt < 1:3511720/71‑1 (MQ=255) tCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGa < 1:2311711/46‑1 (MQ=255) cGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGa > 1:893790/1‑71 (MQ=255) | GTCATTTGACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGA > W3110S.gb/4314956‑4315094 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |