| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,328,660 | C→T | 13.5% | V125I (GTC→ATC) | phnD ← | phosphonate/organophosphate ester transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,328,660 | 0 | C | T | 13.5% | 165.2 / 13.7 | 59 | V125I (GTC→ATC) | phnD | phosphonate/organophosphate ester transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (26/25); new base T (6/2); total (32/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
TTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTGG > W3110S.gb/4328590‑4328728 | ttGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGAc > 1:4124366/1‑71 (MQ=255) aaaGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGATGATc > 1:1304035/1‑70 (MQ=255) ggTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGATGATCAaca > 1:4387421/1‑71 (MQ=255) ggTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAaca > 1:2149417/1‑71 (MQ=255) gTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAacac > 1:365446/1‑71 (MQ=255) agaTCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGc < 1:1333581/70‑1 (MQ=255) agaTCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCt > 1:1507087/1‑71 (MQ=255) agaTCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCt > 1:2090796/1‑71 (MQ=255) cTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCGAg > 1:1478105/1‑71 (MQ=255) cTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAg > 1:4548090/1‑71 (MQ=255) cTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAg > 1:3139489/1‑71 (MQ=255) tCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGATGATCAACACGCTCCAGTaa > 1:3382766/1‑71 (MQ=255) tCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCa < 1:1813743/67‑1 (MQ=255) tCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGt < 1:1288028/69‑1 (MQ=255) tCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTaa > 1:4002605/1‑71 (MQ=255) tCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTaa > 1:3042994/1‑71 (MQ=255) cGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAAcc > 1:4409334/1‑71 (MQ=255) ttCGCCAGCAGATCGTTCAGGTTGTTGATCGGAGTGTCTTTGTTGACGATCAACACGCTCCAGTAAc > 1:360653/1‑67 (MQ=255) cGCCAGCAGATCGTTCAGGGTGTTGATCGGACTGTCTTTGTTGACGATCCACACGCTCCAGTAACCCgg < 1:1668282/69‑1 (MQ=255) gCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCg < 1:1543889/70‑1 (MQ=255) gCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGa < 1:1816068/71‑1 (MQ=255) agcagATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGATGATCAACACGCTCCAGTAACCCGGCGat > 1:3525597/1‑69 (MQ=255) agcagATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGatcc < 1:4374467/71‑1 (MQ=255) gcagATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCa < 1:4001899/45‑1 (MQ=255) gcagATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGatcca < 1:3541070/71‑1 (MQ=255) agATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGatccatc > 1:3978701/1‑71 (MQ=255) gATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGatccatc > 1:3375807/1‑70 (MQ=255) gATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGatccatc > 1:1418856/1‑70 (MQ=255) gATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGatcca > 1:4020322/1‑68 (MQ=255) aTCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATccg > 1:406671/1‑71 (MQ=255) tCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATctgc < 1:1748614/71‑1 (MQ=255) ttCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTcc < 1:3556036/46‑1 (MQ=255) tCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATccg > 1:2940651/1‑66 (MQ=255) cAGGTTGTTGATCGGACTGTCTTTGTTGACGATCaa > 1:2068184/1‑36 (MQ=255) cAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCcgcg < 1:3525188/70‑1 (MQ=255) cAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGa > 1:3133478/1‑71 (MQ=255) tgATCGGACTGTCTTTGTTGATGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTgg > 1:2297984/1‑71 (MQ=255) tgATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCgg > 1:3738721/1‑46 (MQ=255) gATCGGACTGTCTTTGTTGATGATCAACACGCTCCAGTAACCCGGCGatcca < 1:3822904/52‑1 (MQ=255) aTCGGACTGTCTTTGTTGACGATCAACACGCTCGAGTAACCCGGCGATCCATCCGCCGCGACCGTCTgg < 1:2536281/69‑1 (MQ=255) aTCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGc > 1:3272231/1‑71 (MQ=255) tCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCg < 1:26244/71‑1 (MQ=255) tCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGGCTGGGCg < 1:2850416/71‑1 (MQ=255) cGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGc > 1:2347984/1‑43 (MQ=255) ggACTGTCTTTGTTGATGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCg > 1:3959671/1‑69 (MQ=255) gACTGTCTTTGTTGACGATCAACACGCTCCAGTAAcc < 1:3736645/37‑1 (MQ=255) gACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGAcc < 1:1760167/59‑1 (MQ=255) tGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCg < 1:743626/57‑1 (MQ=255) tCTTTGTTGATGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGAcc < 1:2071612/69‑1 (MQ=255) tCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGAcc < 1:4173056/69‑1 (MQ=255) tCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTg < 1:4493859/71‑1 (MQ=255) cTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTgg > 1:811881/1‑71 (MQ=255) tttGTTGACGATCAACACGCTCCAGTAACCCGGCGatccatc > 1:3547589/1‑42 (MQ=255) tttGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAg < 1:3790615/64‑1 (MQ=255) ttgttgACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGc > 1:4290235/1‑70 (MQ=255) tgttgACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGcc < 1:2593741/70‑1 (MQ=255) ttgACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCAt < 1:287404/70‑1 (MQ=255) gACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGt < 1:1746826/48‑1 (MQ=255) aCGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCg > 1:3850428/1‑54 (MQ=255) aCGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTgg < 1:320378/70‑1 (MQ=255) | TTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTGG > W3110S.gb/4328590‑4328728 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |