| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 406,395 | C→T | 13.5% | intergenic (+1/‑257) | yaiA → / → aroM | hypothetical protein/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 406,395 | 0 | C | T | 13.5% | 161.6 / 14.7 | 59 | intergenic (+1/‑257) | yaiA/aroM | hypothetical protein/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (24/27); new base T (5/3); total (29/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.72e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.26e-01 | |||||||||||
AGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCATAAT > W3110S.gb/406326‑406462 | aGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGAcc > 1:2203273/1‑71 (MQ=255)aGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGAcc < 1:1489116/71‑1 (MQ=255) tGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCg > 1:3340552/1‑70 (MQ=255) gAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCa > 1:54380/1‑71 (MQ=255) aaCATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCAt < 1:498175/71‑1 (MQ=255) aCATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCAt < 1:763505/70‑1 (MQ=255) cATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGATCGCATCa < 1:2948472/71‑1 (MQ=255) tCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGATCGCATCAGa > 1:408586/1‑71 (MQ=255) ccGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGCCCGCATCAGa > 1:554242/1‑66 (MQ=255) cTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCgg < 1:3035041/71‑1 (MQ=255) cTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCg > 1:4593856/1‑70 (MQ=255) tCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCgg > 1:470089/1‑70 (MQ=255) tCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCgg > 1:4530151/1‑70 (MQ=255) cAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCgg > 1:3480324/1‑69 (MQ=255) cAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCgg > 1:2778385/1‑69 (MQ=255) aGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCgg < 1:1780585/68‑1 (MQ=255) aGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGa < 1:3821739/69‑1 (MQ=255) cGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTc < 1:537850/71‑1 (MQ=255) gatggatgCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCgg < 1:4492319/61‑1 (MQ=255) gatggatgCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCt < 1:4124598/71‑1 (MQ=255) atggatgCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCa < 1:3820773/47‑1 (MQ=255) tggatgCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGATCGCATCAGACTGCTCGGAAGGGATTCt > 1:2959678/1‑69 (MQ=255) tggatgCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGa < 1:2784589/71‑1 (MQ=255) ggatgCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAgg < 1:4589952/62‑1 (MQ=255) tgCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGAtt < 1:2166253/63‑1 (MQ=255) tgCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCt < 1:307551/65‑1 (MQ=255) cGAAAAAACGCTATGAGGACCCTGACAAAGAGTGATCGCATCAGAc > 1:1221863/1‑46 (MQ=255) cGAAAAAACGCTATGAGGACCCTGACAAAGAGTGATCGCATCAGAc > 1:983427/1‑46 (MQ=255) cGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCa > 1:1345243/1‑71 (MQ=255) gAAAAAACGCTATGAGGACCCTGACAAAGAGTGATCGCATCAGACTGCTCCGAAGGGATTCTGAGTGCCAc > 1:167893/1‑71 (MQ=255) gAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCAc > 1:1749690/1‑71 (MQ=255) aaaaaaCGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGa > 1:3925801/1‑57 (MQ=255) aaaaaaCGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCt < 1:3608934/61‑1 (MQ=255) aaaaaaCGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTg < 1:3157016/62‑1 (MQ=255) aaaaaaCGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTg < 1:470624/62‑1 (MQ=255) aaaaaaCGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCa > 1:2674162/1‑69 (MQ=255) aaaaaaCGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACt < 1:219756/71‑1 (MQ=255) aaaaaCGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTa > 1:1291010/1‑71 (MQ=255) aaaaaCGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTa > 1:3129678/1‑71 (MQ=255) aaaaaCGCTATGAGGACCCTGAAAAAGAGTGACCGCATc > 1:3519500/1‑39 (MQ=255) aaaaCGCTATGAGGACCCTGACAAAGAGTGACCGCATCa > 1:1476987/1‑39 (MQ=255) aaCGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACaa < 1:3265574/71‑1 (MQ=255) aCGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCt < 1:313006/56‑1 (MQ=255) cGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAgg > 1:390342/1‑71 (MQ=255) gCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAggg < 1:2098784/71‑1 (MQ=255) tATGAGGACCCTGACAAAGAGTGATCGCATCAGACTGCTCgg < 1:828657/42‑1 (MQ=255) tATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGAt > 1:295247/1‑71 (MQ=255) tATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGAt > 1:1531807/1‑71 (MQ=255) tGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCt < 1:852284/71‑1 (MQ=255) aGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTg < 1:2666981/70‑1 (MQ=255) ccTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTg < 1:3382645/44‑1 (MQ=255) tGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGAt < 1:4587609/38‑1 (MQ=255) aCAAAGAGTGACCGCATCAGACTGCTCGGAAGGGAtt > 1:2913287/1‑37 (MQ=255) aCAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTc > 1:4030344/1‑38 (MQ=255) aCAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACAttt < 1:263794/71‑1 (MQ=255) aCAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACAttt < 1:2238962/71‑1 (MQ=255) aCAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACAttt > 1:2745365/1‑71 (MQ=255) aCAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACAtt < 1:2891058/70‑1 (MQ=255) aaaGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATATGCGTCACAtttt > 1:3272875/1‑70 (MQ=255) agTGACCGCATCAGACTGCTCGGAAGGGATTCTGAg > 1:1448400/1‑36 (MQ=255) tGATCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCATAAt < 1:3572578/71‑1 (MQ=255) ccGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCg < 1:1963950/52‑1 (MQ=255) | AGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCATAAT > W3110S.gb/406326‑406462 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |