| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 695,215 | C→T | 10.9% | A55T (GCT→ACT) | miaB ← | isopentenyl‑adenosine A37 tRNA methylthiolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 695,215 | 0 | C | T | 10.9% | 134.2 / 6.6 | 46 | A55T (GCT→ACT) | miaB | isopentenyl‑adenosine A37 tRNA methylthiolase |
| Reads supporting (aligned to +/- strand): ref base C (21/20); new base T (2/3); total (23/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
ATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGAT > W3110S.gb/695145‑695283 | aTAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGc > 1:3519191/1‑71 (MQ=255)aTAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGc > 1:3196733/1‑71 (MQ=255) tAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGcc < 1:2005041/71‑1 (MQ=255) cTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTcgcg > 1:1484552/1‑70 (MQ=255) gATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGTCTTCTCGCGGAtt > 1:2586261/1‑71 (MQ=255) gATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGAtt < 1:780166/71‑1 (MQ=255) aTTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGAtt > 1:3204107/1‑70 (MQ=255) ttcttcTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGAtt > 1:53732/1‑69 (MQ=255) tcttctCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGc > 1:2445414/1‑55 (MQ=255) ctctTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGc < 1:3801353/68‑1 (MQ=255) ctctTTTAACAGTTTCAAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAgg > 1:2918102/1‑71 (MQ=255) ttttAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTcgcg < 1:418571/57‑1 (MQ=255) ttAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGa > 1:4404984/1‑57 (MQ=255) ttAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGa > 1:766588/1‑57 (MQ=255) tAACAGTTTCCAGCGACCCAACTGATGGAATACTTTTTCCTGAGTCTtc < 1:2949740/49‑1 (MQ=39) aCAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTcag > 1:4360332/1‑70 (MQ=255) cAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGTCTTCTCGCGGATTGAGCAGgtg < 1:1586059/64‑1 (MQ=255) aGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGTCTTCTCGCGGATTGAGCAGGTGTTcagcag < 1:824381/71‑1 (MQ=255) aGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTcagaag < 1:2962685/71‑1 (MQ=255) gTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTcagcag > 1:3852402/1‑70 (MQ=255) tttCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTtctc < 1:1796967/45‑1 (MQ=255) tttCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTtctc < 1:81443/45‑1 (MQ=255) ttCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTcg < 1:916213/45‑1 (MQ=255) gCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAg > 1:1545592/1‑49 (MQ=255) gCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAg < 1:4003229/52‑1 (MQ=255) ccAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGct > 1:2736116/1‑71 (MQ=255) aaCTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGgtg > 1:1016580/1‑48 (MQ=255) aCTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGcttat > 1:3351497/1‑69 (MQ=255) cTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGa > 1:2270465/1‑39 (MQ=255) cTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTcagcag > 1:3205972/1‑54 (MQ=255) cTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGcttctt < 1:1297944/71‑1 (MQ=255) aGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACg > 1:4553470/1‑71 (MQ=255) gACTTTTTCCTGAGTCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGt > 1:1882285/1‑71 (MQ=255) gACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGt > 1:3415491/1‑71 (MQ=255) gACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGt < 1:437418/71‑1 (MQ=255) gACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGt < 1:4099318/71‑1 (MQ=255) aCTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTcag < 1:134050/41‑1 (MQ=255) aCTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTc < 1:4427814/71‑1 (MQ=255) aCTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTc > 1:3330158/1‑71 (MQ=255) cTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTc < 1:3762728/70‑1 (MQ=255) tttttCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGc > 1:3123114/1‑54 (MQ=255) ttttCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGt > 1:3729296/1‑71 (MQ=255) tttCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTc < 1:2506045/35‑1 (MQ=255) tttCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTcc > 1:3648395/1‑59 (MQ=255) tttCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCg > 1:3090435/1‑68 (MQ=255) tCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCa < 1:154096/70‑1 (MQ=255) tCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCa < 1:145508/70‑1 (MQ=255) tGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATcc < 1:3521544/45‑1 (MQ=255) aGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGa < 1:3909127/70‑1 (MQ=255) aGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGAt < 1:1280768/71‑1 (MQ=255) | ATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGAT > W3110S.gb/695145‑695283 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |