| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 833,307 | G→T | 12.2% | T22T (ACC→ACA) | ybiA ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 833,307 | 0 | G | T | 12.2% | 148.6 / 5.3 | 49 | T22T (ACC→ACA) | ybiA | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (26/17); new base T (2/4); total (28/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01 | |||||||||||
AGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTA > W3110S.gb/833238‑833374 | aGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTgg > 1:2074319/1‑71 (MQ=255) aGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTg < 1:1967055/71‑1 (MQ=255) aGGTTTTCCCGGCAACCTTGATGGGCCAGGCTGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGc < 1:2493643/69‑1 (MQ=255) gTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTa < 1:2808872/71‑1 (MQ=255) ccGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGAAAtt < 1:4177052/71‑1 (MQ=255) ccGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGAAAtt < 1:2257202/71‑1 (MQ=255) tCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTg > 1:3386749/1‑57 (MQ=255) aaCCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTatga > 1:1270620/1‑71 (MQ=255) ccTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTatgatg > 1:2158303/1‑71 (MQ=255) cTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTtgtg > 1:1014305/1‑52 (MQ=255) tGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTa > 1:4344001/1‑55 (MQ=255) aTGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGgtgt < 1:2232125/70‑1 (MQ=255) gCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAAtt < 1:3651628/55‑1 (MQ=255) gCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGc < 1:1165476/71‑1 (MQ=255) ccAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAAAAATTTATGATGGTGTCTTGCa > 1:2971470/1‑71 (MQ=255) gctgcAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCtt > 1:776515/4‑64 (MQ=255) cggcAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATg < 1:907997/68‑1 (MQ=255) ggcAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAAtt > 1:2340334/1‑48 (MQ=255) tcAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATgg < 1:1321071/54‑1 (MQ=255) gcAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATgg < 1:1950657/55‑1 (MQ=255) aaaGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTa > 1:2097557/1‑47 (MQ=255) aaaGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGAc > 1:4352457/1‑66 (MQ=255) aaaGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGAc > 1:2931168/1‑66 (MQ=255) aGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTgg < 1:4480171/71‑1 (MQ=255) aGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTgg < 1:734766/71‑1 (MQ=255) ttAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTatg < 1:210182/45‑1 (MQ=255) ttAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCAt < 1:4501674/59‑1 (MQ=255) ttAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGAt > 1:1838265/1‑71 (MQ=255) aGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACgtgt > 1:1818495/1‑64 (MQ=255) aaaaTCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCtt > 1:4250988/1‑70 (MQ=255) aaaTCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTtg > 1:4105594/1‑71 (MQ=255) tCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGc > 1:361483/1‑71 (MQ=255) cACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCt > 1:3782934/1‑71 (MQ=255) aCCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCt > 1:4064326/1‑70 (MQ=255) ccGTAGTCATCGCTTGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCg < 1:4163272/71‑1 (MQ=255) cGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACgt < 1:2975251/51‑1 (MQ=255) gTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCAt > 1:3784004/1‑45 (MQ=255) gTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCAt > 1:2005432/1‑45 (MQ=255) gTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGaa > 1:2286159/1‑71 (MQ=255) tAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAAc > 1:3843201/1‑71 (MQ=255) aGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCt < 1:1047901/58‑1 (MQ=255) aGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACg > 1:81661/1‑71 (MQ=255) gTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACgg > 1:181750/1‑71 (MQ=255) gTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACg < 1:433999/70‑1 (MQ=255) tCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACgtgt < 1:1444395/45‑1 (MQ=255) cGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAAc > 1:3997771/1‑64 (MQ=255) cGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAAc > 1:4573695/1‑64 (MQ=255) cGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCAt > 1:3537747/1‑70 (MQ=255) gCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCtt > 1:4047183/1‑52 (MQ=255) gCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCtt > 1:4042064/1‑52 (MQ=255) gCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTa < 1:2639962/71‑1 (MQ=255) | AGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTA > W3110S.gb/833238‑833374 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |