| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 862,250 | G→T | 13.8% | T260N (ACT→AAT) | ybiW ← | predicted pyruvate formate lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 862,250 | 0 | G | T | 13.8% | 108.6 / 4.2 | 36 | T260N (ACT→AAT) | ybiW | predicted pyruvate formate lyase |
| Reads supporting (aligned to +/- strand): ref base G (15/16); new base T (3/2); total (18/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01 | |||||||||||
GAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTT > W3110S.gb/862181‑862319 | gAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGt > 1:895961/1‑71 (MQ=255) aGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTc > 1:4142484/1‑71 (MQ=255) gTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCt < 1:1869316/71‑1 (MQ=255) gTGACCGATAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCt < 1:4570931/71‑1 (MQ=255) tGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTg < 1:280741/71‑1 (MQ=255) cGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTgcggc > 1:3771604/1‑71 (MQ=255) cGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTgcg > 1:1856071/1‑69 (MQ=255) ttAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGcggcgg > 1:2445919/1‑71 (MQ=255) tAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCggcggc > 1:2348638/1‑71 (MQ=255) tCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTc > 1:4249415/1‑57 (MQ=255) gATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTggg > 1:4090782/1‑71 (MQ=255) tCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTggg < 1:884071/69‑1 (MQ=255) tCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGc > 1:349244/1‑70 (MQ=255) cTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGa > 1:4537404/1‑71 (MQ=255) cAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAATTCTGCGGCGGCTGGTGGGCGATaa < 1:1434073/71‑1 (MQ=255) cAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGAt > 1:2750299/1‑69 (MQ=255) tCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCg < 1:1821618/71‑1 (MQ=255) gATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGtttt < 1:3223090/71‑1 (MQ=255) gATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGtt < 1:871611/69‑1 (MQ=255) aGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGcc < 1:928550/71‑1 (MQ=255) aGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGcc < 1:923228/71‑1 (MQ=255) gTAACACAGTTGCAGCGCCTGCCAGAAATTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCa < 1:2233826/71‑1 (MQ=255) tAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCg > 1:1895048/1‑57 (MQ=255) acacAGTTGCAGCGCCTGCCAGAAATTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATc > 1:836392/1‑70 (MQ=255) acacAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTTGGCGATAAGATCGCAGTTTTCTGCCATc < 1:4284692/70‑1 (MQ=255) aGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCcag < 1:1748054/71‑1 (MQ=255) cTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGcc < 1:3130481/58‑1 (MQ=255) tGCCAGAAATTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATcgc > 1:3218975/1‑70 (MQ=255) tGCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGcc < 1:2368725/51‑1 (MQ=255) gacAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGc < 1:3192316/53‑1 (MQ=255) gCCAGAAATTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGc > 1:1494127/1‑55 (MQ=255) cAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGtt > 1:1931230/1‑61 (MQ=255) cAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACg < 1:2182326/71‑1 (MQ=255) aaaGTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACCGCt > 1:1672775/1‑71 (MQ=255) aaGTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCtt < 1:2678633/71‑1 (MQ=255) aGTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCttt > 1:646183/1‑71 (MQ=255) | GAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCATCGCCAGCAGTTCATCGCGACGGCTTT > W3110S.gb/862181‑862319 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |