| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 70,740 | T→G | 56.3% | G118G (GGT→GGG) | araC → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 70,740 | 0 | T | G | 56.3% | 87.2 / 66.4 | 73 | G118G (GGT→GGG) | araC | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base T (12/20); new base G (16/25); total (28/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGCCGGGC > W3110S.gb/70671‑70810 | ggTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGt < 1:1858442/70‑1 (MQ=255) gTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgtt < 1:2521600/71‑1 (MQ=255) tttACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGtttc > 1:3095322/1‑71 (MQ=255) ttACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGtttct > 1:2635534/1‑71 (MQ=255) ttACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttct > 1:2080993/1‑71 (MQ=255) ttACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttct > 1:3722871/1‑71 (MQ=255) tACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGtttct < 1:2404697/70‑1 (MQ=255) tACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGtttct < 1:4172983/70‑1 (MQ=255) tACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttct < 1:3683659/70‑1 (MQ=255) tACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttct < 1:2273809/70‑1 (MQ=255) aCTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttcttt > 1:3686390/1‑71 (MQ=255) cTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttcttt < 1:3398636/70‑1 (MQ=255) cTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttcttt < 1:1342530/70‑1 (MQ=255) tCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttcttt > 1:2511885/1‑67 (MQ=255) tCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGcc > 1:3913108/1‑71 (MQ=255) tCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGc < 1:657290/70‑1 (MQ=255) cGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGccc > 1:147424/1‑71 (MQ=255) tCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCg < 1:657772/70‑1 (MQ=255) ccGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGa > 1:1523441/1‑71 (MQ=255) cgcgcgCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGAt > 1:2271346/1‑71 (MQ=255) gcgcgcCTACTGGCATGAATGGCTTAACTGGGCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATg < 1:2759043/71‑1 (MQ=255) gcgcgcCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATg < 1:676036/71‑1 (MQ=255) gcCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGaa > 1:828186/1‑69 (MQ=255) ccTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAgcg < 1:2518535/71‑1 (MQ=255) cTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAgcg > 1:2447978/1‑70 (MQ=255) tACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGtttct > 1:4439108/1‑52 (MQ=255) tACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttcttt < 1:1488448/54‑1 (MQ=255) aCTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCa > 1:1715975/1‑70 (MQ=255) aCTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCAc > 1:556060/1‑71 (MQ=255) aCTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCAc > 1:4035953/1‑71 (MQ=255) gCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATg < 1:424416/59‑1 (MQ=255) gCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGc > 1:2558737/1‑71 (MQ=255) cATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGcc < 1:2611028/71‑1 (MQ=255) cATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGcc < 1:2616519/71‑1 (MQ=255) cATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCCCCCGGATGAAGCGCACCAGcc < 1:2180228/71‑1 (MQ=255) aaTGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGc > 1:3672269/1‑47 (MQ=255) aTGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGcc < 1:1957138/66‑1 (MQ=255) aTGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGcc < 1:1513667/66‑1 (MQ=255) aTGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAt < 1:3358078/70‑1 (MQ=255) tGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt < 1:565242/71‑1 (MQ=255) tGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt < 1:2691028/71‑1 (MQ=255) tGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt < 1:4033009/71‑1 (MQ=255) tGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt < 1:2007686/71‑1 (MQ=255) tGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt < 1:4429268/71‑1 (MQ=255) ggCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt > 1:4248980/1‑70 (MQ=255) gCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAtt > 1:3792262/1‑68 (MQ=255) gCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTc < 1:2315573/70‑1 (MQ=255) cTTAACTGGCCGTCAATATTTTCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAg > 1:1931501/1‑71 (MQ=255) ttAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGc > 1:3099591/1‑71 (MQ=255) aaCTGGCCGTCAATATTTGCCAATACGGGtttctttc > 1:2942912/1‑37 (MQ=255) aaCTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCa < 1:1276909/56‑1 (MQ=255) aaCTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCa > 1:118571/1‑67 (MQ=255) aaCTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCg < 1:2593526/70‑1 (MQ=255) tGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt < 1:834738/62‑1 (MQ=255) ggCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt < 1:3535060/61‑1 (MQ=255) ggCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCa > 1:448169/1‑58 (MQ=255) gCCGTCAATATTTGCCAATACGGGTTTCTTTCGCACGGATGAAGCGCACAAGCCGCATTTCAGCGACATGt < 1:2389920/71‑1 (MQ=255) gCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATg < 1:2547136/41‑1 (MQ=255) ccGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGtt < 1:101410/71‑1 (MQ=255) cGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGttt < 1:2931649/71‑1 (MQ=255) cGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGttt < 1:2431730/71‑1 (MQ=255) cGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGttt < 1:2308199/71‑1 (MQ=255) gTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTg < 1:2102122/71‑1 (MQ=255) tCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTgg < 1:2642947/71‑1 (MQ=255) tCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCAc < 1:3656248/45‑1 (MQ=255) aaTATTTGCCAATACGGGTTTCTTTCGCACGGATGAAGCGCACCAGCCGCATTTCAg > 1:1697854/1‑57 (MQ=255) atatTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCa < 1:3147776/71‑1 (MQ=255) tatTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGc < 1:4589169/56‑1 (MQ=255) atTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAtt < 1:1413502/50‑1 (MQ=255) tttGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGttt > 1:4061481/1‑63 (MQ=255) tttGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGtt < 1:3005772/62‑1 (MQ=255) tttGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCtaa < 1:3649907/70‑1 (MQ=255) ttGCCAATACGGGTTTCTTTCGCTCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAAt < 1:3454961/70‑1 (MQ=255) ttGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAtt < 1:1008307/48‑1 (MQ=255) tGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGAc > 1:742297/1‑55 (MQ=255) tGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGtt < 1:2402513/60‑1 (MQ=255) tGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTg > 1:414097/1‑62 (MQ=255) gCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAt > 1:4611597/1‑45 (MQ=255) ccAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCAt < 1:1498018/70‑1 (MQ=255) ccAATACAGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCAt < 1:86171/70‑1 (MQ=255) cAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCa < 1:1607020/47‑1 (MQ=255) cAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAAt < 1:3314955/66‑1 (MQ=255) aaTACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTaa > 1:1431030/1‑71 (MQ=255) tACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTa > 1:3217747/1‑68 (MQ=255) aCGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTggg > 1:3933858/1‑57 (MQ=255) cGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGc > 1:1856838/1‑44 (MQ=255) cGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTCCAGCGACCTGTTTGGGCaaa > 1:574869/1‑60 (MQ=255) cGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGa > 1:2816931/1‑46 (MQ=255) gggTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCAtt < 1:4101023/64‑1 (MQ=255) gTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCa < 1:1807972/60‑1 (MQ=255) tttctttcGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGCCGGGc < 1:442398/71‑1 (MQ=255) | GGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGCCGGGC > W3110S.gb/70671‑70810 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |