| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,084,947 | A→G | 18.0% | K384E (AAA→GAA) | ycdB → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,084,947 | 0 | A | G | 18.0% | 138.5 / 25.2 | 61 | K384E (AAA→GAA) | ycdB | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (19/31); new base G (6/5); total (25/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACTGGATATGGGGTTGCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGCGGTTATTTTTTTGCGCTGCC > W3110S.gb/1084879‑1085017 | aaCTGGATATGGGGTTGCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACaagaa < 1:3654653/71‑1 (MQ=255) tGGATATGGGGTTGCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAGAAAgg > 1:937130/1‑71 (MQ=255) gATATGGGGTTGCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCt > 1:2661563/1‑71 (MQ=255) atatGGGGTTGCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTc < 1:1593293/71‑1 (MQ=255) tGGGGTTGCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCaa < 1:65278/70‑1 (MQ=255) tGGGGTTGCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCaa < 1:2990540/70‑1 (MQ=255) tGGGGTTGCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGCCTTCCTGACAGTACAAAAAAGGCTCaa < 1:1059031/70‑1 (MQ=255) gggTTGCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATgg > 1:2777977/1‑71 (MQ=255) gTTGCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCg < 1:4269882/71‑1 (MQ=255) gTTGCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATgg < 1:984535/69‑1 (MQ=255) ttGCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGa < 1:829003/71‑1 (MQ=255) gCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCa < 1:4569540/62‑1 (MQ=255) gCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAg > 1:4242193/1‑71 (MQ=255) cTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAg < 1:2397154/70‑1 (MQ=255) tttGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCGAAGCGCt > 1:131689/1‑71 (MQ=255) tttGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCGAAGCGCt > 1:126232/1‑71 (MQ=255) tGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCaa < 1:377674/57‑1 (MQ=255) tGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTg < 1:4490666/70‑1 (MQ=255) tGCTACTAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGa < 1:471958/59‑1 (MQ=255) cTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCGAAGCGCTggagg < 1:3236191/68‑1 (MQ=255) cTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTggagg < 1:2761229/68‑1 (MQ=255) aCCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCt < 1:4436206/61‑1 (MQ=255) ccAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTggagga < 1:1788317/66‑1 (MQ=255) cAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAAt > 1:3513839/1‑48 (MQ=255) aaCACGATCTGGAAAAAGGCTTCCTGACAGTACAAGAAAGGCTCaa < 1:2757156/46‑1 (MQ=255) aaCACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAAt > 1:3108996/1‑66 (MQ=255) aaCACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGt > 1:1069250/1‑70 (MQ=255) acacGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTa < 1:544160/71‑1 (MQ=255) acacGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTa < 1:1943799/71‑1 (MQ=255) cacGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCaa > 1:1838233/1‑44 (MQ=255) cacGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGc > 1:1181408/1‑48 (MQ=255) cacGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAgcgc > 1:1395145/1‑55 (MQ=255) acGATCTGGAAAAAGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTaaa > 1:1255810/1‑71 (MQ=255) acGATCTGGAAAAAGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTaa < 1:2445810/70‑1 (MQ=255) acGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTa > 1:114428/1‑69 (MQ=255) acGATCTGGAAAAAGGCTT‑CTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAAc > 1:2863833/1‑71 (MQ=255) gATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTg < 1:4241157/54‑1 (MQ=255) aTCTGGAAAAAGGCTTCCTGAGAGTACAAAAAAGGCTCATTGGCGAAGCGCTGGAGGATTACGTTa < 1:2137030/66‑1 (MQ=255) tCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAAt < 1:3906274/59‑1 (MQ=255) cTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTAt > 1:4451036/1‑71 (MQ=255) tGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTaaa > 1:2641198/1‑65 (MQ=255) gAAAAAGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTa < 1:1540731/67‑1 (MQ=255) gAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATcg > 1:2568974/1‑70 (MQ=255) aaaaGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGaa > 1:347318/1‑52 (MQ=255) aaaaGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTa < 1:1720367/65‑1 (MQ=255) aaaGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATcggcgg < 1:1604097/71‑1 (MQ=255) aaGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATcggcggc > 1:1797906/1‑71 (MQ=255) aaGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATcggcggc < 1:1243135/71‑1 (MQ=255) cTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGCGGtt < 1:484371/71‑1 (MQ=255) ccTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGCGGTTAt < 1:388968/70‑1 (MQ=255) cTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTggag > 1:3647015/1‑39 (MQ=255) cTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTggag > 1:3763901/1‑39 (MQ=255) cTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGCGGTTAttt < 1:1398116/71‑1 (MQ=255) aCAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGCGGTTAttttt < 1:1308979/70‑1 (MQ=255) aCAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGCGGTTAttttt < 1:2294638/70‑1 (MQ=255) aGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGCGGTTATTTTTTTg < 1:3197758/71‑1 (MQ=255) gTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCt < 1:3210383/48‑1 (MQ=255) tACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGt < 1:1501791/40‑1 (MQ=255) tACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGCGGTTAttttt > 1:1015990/1‑66 (MQ=255) tACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGCGGTTAttttt < 1:1276462/66‑1 (MQ=255) aCAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGCGGTTAttttt > 1:935121/1‑65 (MQ=255) cAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATAc > 1:4252626/1‑36 (MQ=255) aaaaaGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTa > 1:3597580/1‑44 (MQ=255) aaaaaGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGCGGTTATTTTTTTGCGCTGc > 1:94012/1‑71 (MQ=255) aaaaGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGCGGt > 1:2548444/1‑54 (MQ=255) aaaaGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGCGGTTAttttttttg > 1:3962789/1‑63 (MQ=255) aaaaGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGCGGTTATTTTTTTGCGCTg > 1:68869/1‑69 (MQ=255) aaaaGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGCGGTTATTTTTTTGCGCTGcc > 1:3187712/1‑71 (MQ=255) | AACTGGATATGGGGTTGCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGCGGTTATTTTTTTGCGCTGCC > W3110S.gb/1084879‑1085017 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |