| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,099,547 | C→T | 10.5% | A143V (GCC→GTC) | ycdX → | predicted zinc‑binding hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,099,547 | 0 | C | T | 10.5% | 111.1 / 4.1 | 38 | A143V (GCC→GTC) | ycdX | predicted zinc‑binding hydrolase |
| Reads supporting (aligned to +/- strand): ref base C (11/23); new base T (2/2); total (13/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.95e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01 | |||||||||||
CAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTCCTCATTTTTACACTC > W3110S.gb/1099485‑1099613 | cAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTg < 1:1677972/71‑1 (MQ=255) gCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGaa < 1:1713751/71‑1 (MQ=255) tgtgCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAagc < 1:4291409/66‑1 (MQ=255) tgtgCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAag < 1:3654468/65‑1 (MQ=255) cATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGa < 1:663984/70‑1 (MQ=255) tataATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGaaa > 1:4100049/1‑70 (MQ=255) tataATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGaa < 1:3825647/69‑1 (MQ=255) taataaGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGAAACAt < 1:1830529/71‑1 (MQ=255) aataaGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAagcag > 1:341859/1‑59 (MQ=255) aataaGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGa < 1:2243899/65‑1 (MQ=255) ataaGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTg < 1:1655994/52‑1 (MQ=255) ataaGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTg < 1:3370315/52‑1 (MQ=255) ataaGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATc < 1:954108/70‑1 (MQ=255) cATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGaa < 1:41939/48‑1 (MQ=255) aTCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTg < 1:3345207/45‑1 (MQ=255) tCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGgcgc > 1:1521729/1‑71 (MQ=255) tCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGgcgc > 1:3575261/1‑71 (MQ=255) tCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGAGAAACATCAGGTGgcgc > 1:3839133/1‑71 (MQ=255) ccGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAag > 1:2112208/1‑45 (MQ=255) ccGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGa < 1:1730120/54‑1 (MQ=255) ggAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTgg > 1:522168/1‑70 (MQ=255) ggAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTgg > 1:4064040/1‑70 (MQ=255) ggAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGa < 1:3498783/71‑1 (MQ=255) ccAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCaa > 1:3856590/1‑70 (MQ=255) ccAAATATGAAATAGATGAGAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGgcgc < 1:1825884/60‑1 (MQ=255) cAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGcc < 1:3248480/40‑1 (MQ=255) aaaTATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCaataa < 1:3408622/71‑1 (MQ=255) aaTATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATc > 1:584715/1‑48 (MQ=255) tGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTg > 1:330255/1‑49 (MQ=255) gAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCa < 1:2168050/61‑1 (MQ=255) aTGTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTCCTCATTTTTa < 1:2769908/71‑1 (MQ=255) aTGTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGCCGCTGGAAATCAATAATTCCTCAttt < 1:177377/68‑1 (MQ=255) gAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATc < 1:2349857/49‑1 (MQ=255) aaaGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCt > 1:1208376/1‑41 (MQ=255) aaaGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTg < 1:2267179/42‑1 (MQ=255) aaaGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCaataat > 1:2728942/1‑54 (MQ=255) aaaGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTCCTCATTTTTACACTc < 1:1169656/71‑1 (MQ=255) gCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAAt < 1:944372/44‑1 (MQ=255) | CAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTCCTCATTTTTACACTC > W3110S.gb/1099485‑1099613 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |