| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,112,202 | T→C | 18.2% | F431L (TTT→CTT) | mdoG → | glucan biosynthesis protein, periplasmic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,112,202 | 0 | T | C | 18.2% | 69.9 / 11.5 | 33 | F431L (TTT→CTT) | mdoG | glucan biosynthesis protein, periplasmic |
| Reads supporting (aligned to +/- strand): ref base T (13/14); new base C (3/3); total (16/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01 | |||||||||||
TCAACGGGGGATGTGAAGCAGTCGAACCTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATTTTACCGGCGCAGAGATGAAAAAACTGCCAGAGGATACCCCGGTCACAGCGCAAACCAGCATTG > W3110S.gb/1112133‑1112265 | tCAACGGGGGATGTGAAGCAGTCGAACCTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGAttt > 1:1097231/1‑71 (MQ=255) cAACGGGGGATGTGAAGCAGTCGAACCTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGAttt < 1:769354/70‑1 (MQ=255) cAACGGGGGATGTGAAGCAGTCGAACCTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGAttt > 1:1479918/1‑70 (MQ=255) aaCGGGGGATGTGAAGCAGTCGAACCTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGAtctt < 1:2816408/70‑1 (MQ=255) cGGGGGATGTGAAGCAGTCGAACCTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATTTTAcc < 1:2185357/71‑1 (MQ=255) cGGGGGATGTGAAGCAGTCGAACCTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATCTTAcc > 1:3648251/1‑71 (MQ=255) gggggATGTGAAGCAGTCGAACCTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATTTTACCg < 1:152441/71‑1 (MQ=255) aGCAGTCGAACCTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATTTTACCGGCGCAGAGAt > 1:4249239/1‑70 (MQ=255) aGCAGTCGAACCTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATTTTACCGGCGCAGAGATg < 1:3892481/71‑1 (MQ=255) aGTCGAACCTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATTTTACCGGCGCagag > 1:1967106/1‑65 (MQ=255) tCGAACCTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGAttt < 1:1889441/50‑1 (MQ=255) tCGAACCTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATCTTACCGGCGCAGAGATGaaa < 1:505406/69‑1 (MQ=255) gAACCTGATTCGCCAGCCTGACGGTACTATCGCCTTTTTGGTCGATCTTAc > 1:3717523/1‑51 (MQ=255) gAACCTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATTTTACCGGCGCAGAGATGaa > 1:3484974/1‑66 (MQ=255) gAACCTGATTCGCCAGCCTGACGGTACTATCGCCTTTATGGTCGATCTTACCGGCGCAGAGATGaaaaaa < 1:2960921/70‑1 (MQ=255) aaCCTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATTTTACCGGCGCagag < 1:1698283/60‑1 (MQ=255) aCCTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATTTTAc > 1:353618/1‑49 (MQ=255) aCCTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATTTTACCGGCGCAGAGATGAAAAAACTg > 1:1234099/1‑71 (MQ=255) ccTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATTTTACCGGCGCAGAGATGAAAAAACTGc < 1:344828/71‑1 (MQ=255) cTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATTTTACCGGCGCAGAGATGa < 1:2920584/61‑1 (MQ=255) tGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATCTTa < 1:1947911/45‑1 (MQ=255) gATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATTTTACCGGCGCAGAGATGAAAAAACTGc > 1:1968600/1‑68 (MQ=255) gCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATTTTACCGGCGCAGAGATGAAAAAACTGCCAGAGGa < 1:1360334/70‑1 (MQ=255) gCCTGACGGTACTATCGCCTTTGTGGTCGATTTTACCGGCGCAGAGATGAAAAAACTGCCAGAGGATAccc > 1:3539523/1‑71 (MQ=255) gCCTGACGGTACTATCGCCTTTGTGGTCGATTTTACCGGCGCAGAGATGAAAAAACTGCCAGAGGATAc < 1:4443471/69‑1 (MQ=255) gCCTGACGGTACTATCGCCTTTGTGGTCGATCTTAc > 1:2699247/1‑36 (MQ=255) ccTGACGGTACTATCGCCTTTGTGGTCGATTTTACCGGCGCAGAGATGAAAAAACTGCCAGAGGATAccc < 1:3978331/70‑1 (MQ=255) cTGACGGTACTATCGCCTTTGTGGTCGATTTTACCGGCGCAGAGATGa < 1:635508/48‑1 (MQ=255) ggTACTATCGCCTTTGTGGTCGATTTTACCGGCGCAGAGATGAAAAAACTGCCAGAGGATACCCCGGTca > 1:4242756/1‑70 (MQ=255) tACTATCGCCTTTGTGGTCGATTTTACCGGCGCAGAGATGAAAAAACTGCCAGAGGATACCCCGGTCACAg > 1:4608646/1‑71 (MQ=255) aCTATCGCCTTTGTGGTCGATTTTACCGGCGCAGAGATGAAAAAACTGCCAGAGGATACCCCGGTCACAgc > 1:3752415/1‑71 (MQ=255) tATCGCCTTTGTGGTCGATTTTACCGGCGCAGAGATGAAAAAACTGCCagag < 1:2321914/52‑1 (MQ=255) gCCTTTGTGGTCGATTTTACCGGCGCAGAGATGAAAAAACTGCCAGAGGATACCCCGGTCACAgcgc > 1:2538274/1‑67 (MQ=255) ccTTTGTGGTCGATTTTACCGGCGCAGAGATGAAAAAACTGCCa < 1:470921/44‑1 (MQ=255) cTTTGTGGTCGATTTTACCGGCGCAGAGATGAAAAAACTGCCAGAGGATACCCCGGTCACAGCGCAAAc > 1:4435430/1‑69 (MQ=255) tgtgGTCGATTTTACCGGCGCAGAGATGAAAAAACTGCCAGAGGa > 1:3193271/1‑45 (MQ=255) tgtgGTCGATTTTACCGGCGCAGAGATGAAAAAACTGCCAGAGGATACCCCGGTCACAGCGCAAACCAGc < 1:2676491/70‑1 (MQ=255) gTCGATTTTACCGGCGCAGAGATGAAAAAACTGCCAGAGGATACCCCGGTCACAGCGCAAACCAGCATTg < 1:901982/70‑1 (MQ=255) | TCAACGGGGGATGTGAAGCAGTCGAACCTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATTTTACCGGCGCAGAGATGAAAAAACTGCCAGAGGATACCCCGGTCACAGCGCAAACCAGCATTG > W3110S.gb/1112133‑1112265 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |