| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,168,595 | G→T | 8.0% | A312S (GCT→TCT) | ndh → | respiratory NADH dehydrogenase 2/cupric reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,168,595 | 0 | G | T | 8.0% | 243.7 / 4.4 | 74 | A312S (GCT→TCT) | ndh | respiratory NADH dehydrogenase 2/cupric reductase |
| Reads supporting (aligned to +/- strand): ref base G (38/30); new base T (3/3); total (41/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.24e-01 | |||||||||||
GAAACTAACCGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGTTCCGCCGCGTGCTCAGGCTG > W3110S.gb/1168526‑1168661 | gAAACTAACCGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACg < 1:4604475/70‑1 (MQ=255)gAAACTAACCGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGc > 1:793737/1‑71 (MQ=255)gAAACTAACCGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGc > 1:4583597/1‑71 (MQ=255)gAAACTAACCGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGc > 1:4578869/1‑71 (MQ=255) aaCTAACCGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTa < 1:123399/71‑1 (MQ=255) aaCCGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACTCTATTgg > 1:1104836/1‑71 (MQ=255) aCCGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTg > 1:3247264/1‑69 (MQ=255) aCCGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTg > 1:1627155/1‑69 (MQ=255) aCCGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGc < 1:4163623/71‑1 (MQ=255) ccGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCg < 1:3360932/71‑1 (MQ=255) cGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGa < 1:1419375/71‑1 (MQ=255) cGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGa < 1:2672995/71‑1 (MQ=255) aTCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTg > 1:2074396/1‑71 (MQ=255) tCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCt < 1:4038784/59‑1 (MQ=255) tCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACt > 1:404374/1‑69 (MQ=255) tCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTgc > 1:1392689/1‑71 (MQ=255) ccAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGt > 1:360792/1‑71 (MQ=255) cAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGt > 1:28980/1‑70 (MQ=255) gCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACTCTATTGGCGACTGCGCGTCAt < 1:3498656/71‑1 (MQ=255) gtggtggAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGccc < 1:613739/71‑1 (MQ=255) gtggtggAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGcc > 1:4295914/1‑70 (MQ=255) ggtggAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCcgc > 1:1215916/1‑71 (MQ=255) gAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTcc > 1:1006857/1‑71 (MQ=255) gAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTc < 1:606551/70‑1 (MQ=255) aCCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTg > 1:4372980/1‑44 (MQ=255) aCCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTg > 1:4259936/1‑44 (MQ=255) aCCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGc < 1:3842788/46‑1 (MQ=255) aCCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCgg < 1:1626219/71‑1 (MQ=255) aCCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCgg < 1:1803611/71‑1 (MQ=255) ccGACGCTGCAAACCACCGGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGa < 1:3572155/71‑1 (MQ=255) gACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAg < 1:178621/71‑1 (MQ=255) cGCTGCAAACCACCCGCGATCCAGACATTTACTCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAggg < 1:4184410/71‑1 (MQ=255) cGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAggg > 1:67419/1‑71 (MQ=255) cGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAggg > 1:4131997/1‑71 (MQ=255) gCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTc < 1:563816/51‑1 (MQ=255) gCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAgggg > 1:4481612/1‑71 (MQ=255) cTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGc < 1:2672714/39‑1 (MQ=255) cTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGc < 1:1006751/54‑1 (MQ=255) tGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGc > 1:2325822/1‑71 (MQ=255) cAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATg > 1:1265337/1‑50 (MQ=255) cAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCtt > 1:259686/1‑71 (MQ=255) cAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCt < 1:277424/70‑1 (MQ=255) aaaCCACCCGCGATCCAGACATTTACGCTATTGGc < 1:775178/35‑1 (MQ=255) aaaCCACCCGCGATCCAGACATTTACGCTATTGGCGa < 1:1391345/37‑1 (MQ=255) aaaCCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCgg < 1:428813/60‑1 (MQ=255) aaaCCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCgg < 1:423239/60‑1 (MQ=255) aaaCCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCgg < 1:2248938/60‑1 (MQ=255) aaCCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTg > 1:4433116/1‑71 (MQ=255) aaCCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTg < 1:1055550/71‑1 (MQ=255) accaccCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGt > 1:1874932/1‑71 (MQ=255) caccCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCg < 1:2484236/55‑1 (MQ=255) caccCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAgg > 1:4157000/1‑60 (MQ=255) caccCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGTTc < 1:3822174/71‑1 (MQ=255) accCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCcg > 1:939454/1‑48 (MQ=255) accCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCcg > 1:2422015/1‑48 (MQ=255) accCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCcg > 1:2094122/1‑48 (MQ=255) accCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGTTcc > 1:793909/1‑71 (MQ=255) ccGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGTTccgc > 1:1509566/1‑71 (MQ=255) gcgATCCAGACATTTACTCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGTTcc > 1:4201566/1‑67 (MQ=255) gcgATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGTTccgccg < 1:429160/71‑1 (MQ=255) aTCCAGACATTTACTCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCtt < 1:2814215/58‑1 (MQ=255) aTCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGaa > 1:932554/1‑50 (MQ=255) tCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGTTCCGCCGCGTg > 1:3637222/1‑71 (MQ=255) cAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGaa > 1:1416214/1‑47 (MQ=255) cAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAggg > 1:4384868/1‑50 (MQ=255) cAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGc < 1:1029917/53‑1 (MQ=255) cAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGTTCCGCCGCGTGCt > 1:218082/1‑71 (MQ=255) gACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGTTCCGCCGCGTGCTCa < 1:648648/71‑1 (MQ=255) aCATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAggggg > 1:300627/1‑49 (MQ=255) aCATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGTTccgccg > 1:1259120/1‑62 (MQ=255) cATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGTTCCGCCGCGTGCTCAgg < 1:2633238/71‑1 (MQ=255) aTTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGaa > 1:259120/1‑42 (MQ=255) aTTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGTTCCGCCGCGTGCTCAGGc < 1:3918266/71‑1 (MQ=255) tACTCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCttt > 1:2912188/1‑48 (MQ=255) tACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGTTCCGCCGCGTGCTCAGGCTg > 1:3171558/1‑70 (MQ=255) | GAAACTAACCGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGTTCCGCCGCGTGCTCAGGCTG > W3110S.gb/1168526‑1168661 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |