| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,224,919 | G→A | 7.5% | intergenic (‑423/‑123) | ymgG ← / → ymgH | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,224,919 | 0 | G | A | 7.5% | 168.0 / 3.5 | 53 | intergenic (‑423/‑123) | ymgG/ymgH | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (23/26); new base A (2/2); total (25/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
GATATTCATCAGCAACGATTACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAGCGCACTGCGTGTAAAAA > W3110S.gb/1224851‑1224986 | gaTATTCATCAGCAACGATTACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTa > 1:2303994/1‑71 (MQ=255) atTCATCAGCAACGATTACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGa > 1:4581760/1‑71 (MQ=255) caGCAACGATTACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGta > 1:345771/1‑70 (MQ=255) gCAACGATTACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGtatta < 1:2428986/71‑1 (MQ=255) gATTACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAg > 1:3291733/1‑70 (MQ=255) aTTACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGaa < 1:2685975/71‑1 (MQ=255) ttACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACa > 1:3117156/1‑58 (MQ=255) aCATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGta > 1:1834425/1‑59 (MQ=255) cATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTAGGACAGTATTATCAGAAAag < 1:337039/70‑1 (MQ=255) gTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGa < 1:1250380/70‑1 (MQ=255) gTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGa < 1:863122/70‑1 (MQ=255) cATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGAttt > 1:3566041/1‑71 (MQ=255) ttttattttGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGtatt < 1:3177254/51‑1 (MQ=255) ttattttGCCGACGGCCTCATTGTCGAAAGATAAGCGTAc > 1:388365/1‑40 (MQ=255) ttattttGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGaa < 1:2573700/56‑1 (MQ=255) ttattttGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGAtttttt < 1:2176622/70‑1 (MQ=255) ttattttGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTa > 1:1144001/1‑71 (MQ=255) ttattttGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGTATTATCAGAAAAGAGTGATTTTTTa > 1:3113394/1‑71 (MQ=255) attttGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATc > 1:3159854/1‑71 (MQ=255) ttttGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGAttttt < 1:644116/66‑1 (MQ=255) tGCCGACGGCCTCATTGTCGAAAGATAAGAGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAAc > 1:3185368/1‑71 (MQ=255) ccGACGGCCTCATTGTCGAAAGATAAGCATACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACt < 1:3407922/70‑1 (MQ=255) cGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCaa > 1:2300986/1‑67 (MQ=255) aCGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGaaaa < 1:1355052/47‑1 (MQ=255) aCGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTacac > 1:367901/1‑71 (MQ=255) aCGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTacac > 1:1376402/1‑71 (MQ=255) cGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACt > 1:4009834/1‑71 (MQ=255) gCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGAtttt < 1:2125909/54‑1 (MQ=255) ccTCATTGTCGAAAGATAAGCGTACGACAGtattat < 1:2525986/36‑1 (MQ=255) ccTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGAttttt > 1:2600325/1‑54 (MQ=255) cTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGaa < 1:1162030/40‑1 (MQ=255) tCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGAtttttt > 1:817655/1‑53 (MQ=255) cATTGTCGAAAGATAAGCGTACGACAGTATTATCAGaa < 1:2792172/38‑1 (MQ=255) cATTGTCGAAAGATAAGCGTACGACAGTATTATCAGaa < 1:220278/38‑1 (MQ=255) cATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAgcg > 1:2414267/1‑71 (MQ=255) aTTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAgcgc < 1:335325/71‑1 (MQ=255) aTTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAgc < 1:1225221/69‑1 (MQ=255) tGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAGCGCAc > 1:4176830/1‑71 (MQ=255) gTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCaa > 1:1929358/1‑54 (MQ=255) gTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAGCGCAc < 1:2598176/70‑1 (MQ=255) gTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAGCGCACt > 1:1181154/1‑71 (MQ=255) gTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAGCGCACt > 1:370740/1‑71 (MQ=255) tCGAAAGATAAGCGTACGACAGTATTATCAGaaaa < 1:20477/35‑1 (MQ=255) tCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACtt < 1:185676/61‑1 (MQ=255) tCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACtt < 1:1644721/61‑1 (MQ=255) cGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGAtttttt > 1:2508966/1‑46 (MQ=255) gAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAGCGCACTGCg < 1:2496130/71‑1 (MQ=255) aaaGATAAGCATACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACtt < 1:2677999/58‑1 (MQ=255) gATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAGCGCACTGCGTGTa < 1:4397477/71‑1 (MQ=255) aaGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAGCGCACTGCGTGTaaa > 1:3588280/1‑70 (MQ=255) aaGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAGCGCACTGCGTGTaa < 1:4072723/69‑1 (MQ=255) aGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAGCGCACTGCGTGTaaaaa < 1:1574449/71‑1 (MQ=255) aGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAGCGCACTGCGTGTaaaaa < 1:2668209/71‑1 (MQ=255) gTACGACAGTATTATCAGAAAAGAGTGATTATTTATCCAACTACACTTCAGCGCACt < 1:3236337/57‑1 (MQ=255) | GATATTCATCAGCAACGATTACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAGCGCACTGCGTGTAAAAA > W3110S.gb/1224851‑1224986 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |