Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 1,303,631 C→T 100% Q246Y (CAG→TAT)  oppA → oligopeptide transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,303,6310CT100.0% 196.9 / NA 65Q246Y (CAG→TAT) oppAoligopeptide transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (37/28);  total (37/28)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAAT  >  W3110S.gb/1303568‑1303695
                                                               |                                                                
aacgaacgAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAc                                                           >  1:267217/1‑71 (MQ=255)
 acgaacgaATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAcc                                                          >  1:3016121/1‑71 (MQ=255)
 acgaacgaATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAcc                                                          >  1:2027347/1‑71 (MQ=255)
   gaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa                                                        <  1:286724/71‑1 (MQ=255)
     acgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAtt                                                      >  1:1680898/1‑71 (MQ=255)
     acgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAtt                                                      <  1:2333385/71‑1 (MQ=255)
       gaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAttt                                                     <  1:148863/70‑1 (MQ=255)
         aTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc                                                  >  1:281804/1‑71 (MQ=255)
          tCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAttt                                                     <  1:4518718/67‑1 (MQ=255)
           cGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCTAAAACCGTTATTAACTATGTAACCTATTTGCCTa                                                >  1:2095544/1‑71 (MQ=255)
           cGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTaa                                                            <  1:3391765/59‑1 (MQ=255)
           cGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAttt                                                     <  1:3030878/66‑1 (MQ=255)
            gTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTAt                                               >  1:104152/1‑71 (MQ=255)
              tCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTg                                             >  1:2857968/1‑71 (MQ=255)
               cTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGc                                            >  1:3505350/1‑71 (MQ=255)
                ttGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTg                                             >  1:67333/1‑69 (MQ=255)
                 tGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa                                                        <  1:95693/57‑1 (MQ=255)
                  gAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTc                                         >  1:3856587/1‑71 (MQ=255)
                  gAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTc                                         >  1:2065140/1‑71 (MQ=255)
                  gAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTc                                         >  1:923491/1‑71 (MQ=255)
                   aaCGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTc                                         <  1:1841296/70‑1 (MQ=255)
                   aaCGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCt                                        <  1:703471/71‑1 (MQ=255)
                   aaCGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCt                                        <  1:1860911/71‑1 (MQ=255)
                    aCGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTg                                       <  1:2668707/71‑1 (MQ=255)
                     cGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa                                      <  1:3913152/71‑1 (MQ=255)
                     cGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa                                      <  1:1985816/71‑1 (MQ=255)
                      gCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTg                                       >  1:860012/1‑69 (MQ=255)
                      gCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGaa                                     <  1:1920564/71‑1 (MQ=255)
                        aGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAttt                                                     <  1:1671935/53‑1 (MQ=255)
                          cccGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAc                                                           >  1:1718314/1‑45 (MQ=38)
                          cccGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCt                                                 <  1:988434/55‑1 (MQ=255)
                            cGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc                               >  1:565869/1‑71 (MQ=255)
                              aCCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa                                      >  1:1069999/1‑62 (MQ=255)
                               ccTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGAt                            <  1:428125/71‑1 (MQ=255)
                               ccTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACATATTTGCCTATTGCTTCTGAAGTTAc                                >  1:3803156/1‑67 (MQ=255)
                                 tACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCtt                                          <  1:3361755/55‑1 (MQ=255)
                                  aCTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTa                                                <  1:2073170/48‑1 (MQ=39)
                                  aCTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGt                          >  1:4420637/1‑70 (MQ=255)
                                   cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGt                                   >  1:1686993/1‑60 (MQ=255)
                                   cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTc                         >  1:1928374/1‑70 (MQ=255)
                                    tGGAACAACGCGAAAACCGTTATTAACTATGTAAc                                                           >  1:339541/1‑35 (MQ=37)
                                    tGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGaa                                     >  1:3909872/1‑57 (MQ=255)
                                      gAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCa                        >  1:2676618/1‑68 (MQ=255)
                                      gAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCa                        >  1:2038400/1‑68 (MQ=255)
                                       aacaacGCGAAAACCGTTATTAACTATGTAACCTAt                                                       <  1:669851/36‑1 (MQ=37)
                                        acaacGCGAAAACCGTTATTAACTATGTAACCTAttt                                                     >  1:382108/1‑37 (MQ=37)
                                           acGCGAAAACCGTTATTAACTATGTAACCTATTTGc                                                   <  1:403092/36‑1 (MQ=37)
                                            cgcgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCtt                                          >  1:198735/1‑44 (MQ=38)
                                             gcgAAAACCGTTATTAACTATGTAACCTATTTGCCt                                                 >  1:3279192/1‑36 (MQ=37)
                                             gcgAAAACCGTTATTAACTATGTAACCTATTTGCCTATAGCTTc                                         >  1:2670812/1‑44 (MQ=37)
                                              cgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCg                              <  1:1846318/54‑1 (MQ=255)
                                              cgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCg              >  1:929626/1‑70 (MQ=255)
                                              cgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGt             <  1:4420616/71‑1 (MQ=255)
                                               gAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTa            <  1:1874817/71‑1 (MQ=255)
                                                aaaaCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGAt                            >  1:2502684/1‑54 (MQ=255)
                                                aaaaCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAg           <  1:2926871/71‑1 (MQ=255)
                                                 aaaCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAgt          >  1:4575722/1‑71 (MQ=255)
                                                 aaaCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAgt          >  1:1451853/1‑71 (MQ=255)
                                                  aaCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAgtg         <  1:2240326/71‑1 (MQ=255)
                                                  aaCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAgtg         <  1:695263/71‑1 (MQ=255)
                                                  aaCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAgtg         >  1:2164572/1‑71 (MQ=255)
                                                      gTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAgtggtg      >  1:4389396/1‑70 (MQ=255)
                                                         attaACTATGTAACCTATTTGCCTATTGCTTCTGAAg                                    >  1:2035849/1‑37 (MQ=37)
                                                         attaACTATGTAACCTATTTGCCTATTGCTTCTGAAg                                    >  1:352221/1‑37 (MQ=37)
                                                          ttaACTATGTATCCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAAt  <  1:832251/70‑1 (MQ=255)
                                                               |                                                                
AACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAAT  >  W3110S.gb/1303568‑1303695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: