Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 1,389,999 A→G 11.4% intergenic (+24/+20) tyrR → / ← tpx DNA‑binding transcriptional dual regulator, tyrosine‑binding/lipid hydroperoxide peroxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,389,9990AG11.4% 102.7 / 7.5 44intergenic (+24/+20)tyrR/tpxDNA‑binding transcriptional dual regulator, tyrosine‑binding/lipid hydroperoxide peroxidase
Reads supporting (aligned to +/- strand):  ref base A (24/15);  new base G (3/2);  total (27/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.32e-01

GCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGA  >  W3110S.gb/1389936‑1390068
                                                               |                                                                     
gCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCAt                                                                 <  1:1381479/70‑1 (MQ=255)
gCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCAt                                                                 >  1:1384055/1‑70 (MQ=255)
      atatGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCa                                                           >  1:2650043/1‑70 (MQ=255)
       tatGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCAt                                                                 <  1:1468959/63‑1 (MQ=255)
         tGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCatat                                                        >  1:3676295/1‑70 (MQ=255)
            tCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTaaa                                                    <  1:1893298/71‑1 (MQ=255)
             cTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAAt                                                   >  1:4137431/1‑71 (MQ=255)
                 gTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTa                                                 <  1:1460806/69‑1 (MQ=255)
                   cAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCt                                             >  1:1671007/1‑71 (MQ=255)
                   cAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCt                                             >  1:3472183/1‑71 (MQ=255)
                      aagaagaaCGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCttt                                           >  1:1943202/1‑70 (MQ=255)
                       agaagaaCGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCttt                                           >  1:103343/1‑69 (MQ=255)
                       agaagaaCGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCttt                                           >  1:684899/1‑69 (MQ=255)
                          agaaCGAAGAGTAAGCGCGAATATGCCTGATGGTGTTGCACCATCa                                                               >  1:3129599/1‑46 (MQ=18)
                           gaaCGAAGAGTAAGCGCGAATATGCCTGATGGTGTTGCACCAt                                                                 <  1:577485/43‑1 (MQ=18)
                           gaaCGAAGAGTAAGCGCGAATATGCCTGATGGTGCAaca                                                                     <  1:4064854/39‑1 (MQ=255)
                             aCGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACAcc                                                                   <  1:1232634/39‑1 (MQ=255)
                              cGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGc                                  >  1:3739188/1‑71 (MQ=255)
                               gAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGt                                       <  1:2408331/65‑1 (MQ=255)
                               gAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGcc                                 <  1:3432926/71‑1 (MQ=255)
                                aaGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATg                                               >  1:1397493/1‑56 (MQ=255)
                                 agagTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGc                                  >  1:1420350/1‑68 (MQ=255)
                                     tAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAg                                        <  1:1794417/58‑1 (MQ=255)
                                     tAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCagag                             >  1:3965055/1‑69 (MQ=255)
                                       aGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATAttta                                                     >  1:958078/1‑41 (MQ=255)
                                        gcgcGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAgctgct                        >  1:3177004/1‑71 (MQ=255)
                                          gcgAATATGCCTGATGGTGCAACACCATCAGGCATATTa                                                      <  1:3781093/39‑1 (MQ=255)
                                           cgAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCg                     <  1:4372221/71‑1 (MQ=255)
                                              atatGCCTGATGGTGCAACACCATCAGGCATATTAATTTATGCTTTCAGTACAGCCAGAGCTGCTTCGTaa                  >  1:2642995/1‑71 (MQ=255)
                                                   ccTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCgg             >  1:4521353/1‑71 (MQ=255)
                                                    cTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAgctgct                        <  1:2826277/59‑1 (MQ=255)
                                                    cTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCgg             >  1:1169626/1‑70 (MQ=255)
                                                     tGATGGTGTTGCACCATCAGGCATATTAAATTATGCTTTc                                          <  1:3026179/40‑1 (MQ=14)
                                                     tGATGGTGTTGCACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATc                >  1:1106220/1‑66 (MQ=37)
                                                     tGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCt           >  1:898251/1‑71 (MQ=255)
                                                      gATGGTGTTGCACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTc          >  1:249892/1‑71 (MQ=38)
                                                      gATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGt                                       <  1:1448272/42‑1 (MQ=255)
                                                      gATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTc          >  1:2174423/1‑71 (MQ=255)
                                                      gATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTc          >  1:1943294/1‑71 (MQ=255)
                                                       aTGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCg         <  1:1666450/71‑1 (MQ=255)
                                                       aTGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCg         >  1:3146873/1‑71 (MQ=255)
                                                            gCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAgctgc                         <  1:3716667/50‑1 (MQ=255)
                                                            gCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCggtggt    >  1:806553/1‑71 (MQ=255)
                                                            gCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCggtggt    >  1:1076641/1‑71 (MQ=255)
                                                             cAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCTTAATCCGGCTCggtggt    <  1:1554794/70‑1 (MQ=255)
                                                              aaCACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGa  >  1:1875520/1‑71 (MQ=255)
                                                               |                                                                     
GCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGA  >  W3110S.gb/1389936‑1390068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: