| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,389,999 | A→G | 11.4% | intergenic (+24/+20) | tyrR → / ← tpx | DNA‑binding transcriptional dual regulator, tyrosine‑binding/lipid hydroperoxide peroxidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,389,999 | 0 | A | G | 11.4% | 102.7 / 7.5 | 44 | intergenic (+24/+20) | tyrR/tpx | DNA‑binding transcriptional dual regulator, tyrosine‑binding/lipid hydroperoxide peroxidase |
| Reads supporting (aligned to +/- strand): ref base A (24/15); new base G (3/2); total (27/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.32e-01 | |||||||||||
GCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGA > W3110S.gb/1389936‑1390068 | gCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCAt < 1:1381479/70‑1 (MQ=255)gCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCAt > 1:1384055/1‑70 (MQ=255) atatGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCa > 1:2650043/1‑70 (MQ=255) tatGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCAt < 1:1468959/63‑1 (MQ=255) tGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCatat > 1:3676295/1‑70 (MQ=255) tCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTaaa < 1:1893298/71‑1 (MQ=255) cTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAAt > 1:4137431/1‑71 (MQ=255) gTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTa < 1:1460806/69‑1 (MQ=255) cAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCt > 1:1671007/1‑71 (MQ=255) cAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCt > 1:3472183/1‑71 (MQ=255) aagaagaaCGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCttt > 1:1943202/1‑70 (MQ=255) agaagaaCGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCttt > 1:103343/1‑69 (MQ=255) agaagaaCGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCttt > 1:684899/1‑69 (MQ=255) agaaCGAAGAGTAAGCGCGAATATGCCTGATGGTGTTGCACCATCa > 1:3129599/1‑46 (MQ=18) gaaCGAAGAGTAAGCGCGAATATGCCTGATGGTGTTGCACCAt < 1:577485/43‑1 (MQ=18) gaaCGAAGAGTAAGCGCGAATATGCCTGATGGTGCAaca < 1:4064854/39‑1 (MQ=255) aCGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACAcc < 1:1232634/39‑1 (MQ=255) cGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGc > 1:3739188/1‑71 (MQ=255) gAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGt < 1:2408331/65‑1 (MQ=255) gAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGcc < 1:3432926/71‑1 (MQ=255) aaGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATg > 1:1397493/1‑56 (MQ=255) agagTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGc > 1:1420350/1‑68 (MQ=255) tAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAg < 1:1794417/58‑1 (MQ=255) tAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCagag > 1:3965055/1‑69 (MQ=255) aGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATAttta > 1:958078/1‑41 (MQ=255) gcgcGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAgctgct > 1:3177004/1‑71 (MQ=255) gcgAATATGCCTGATGGTGCAACACCATCAGGCATATTa < 1:3781093/39‑1 (MQ=255) cgAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCg < 1:4372221/71‑1 (MQ=255) atatGCCTGATGGTGCAACACCATCAGGCATATTAATTTATGCTTTCAGTACAGCCAGAGCTGCTTCGTaa > 1:2642995/1‑71 (MQ=255) ccTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCgg > 1:4521353/1‑71 (MQ=255) cTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAgctgct < 1:2826277/59‑1 (MQ=255) cTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCgg > 1:1169626/1‑70 (MQ=255) tGATGGTGTTGCACCATCAGGCATATTAAATTATGCTTTc < 1:3026179/40‑1 (MQ=14) tGATGGTGTTGCACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATc > 1:1106220/1‑66 (MQ=37) tGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCt > 1:898251/1‑71 (MQ=255) gATGGTGTTGCACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTc > 1:249892/1‑71 (MQ=38) gATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGt < 1:1448272/42‑1 (MQ=255) gATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTc > 1:2174423/1‑71 (MQ=255) gATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTc > 1:1943294/1‑71 (MQ=255) aTGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCg < 1:1666450/71‑1 (MQ=255) aTGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCg > 1:3146873/1‑71 (MQ=255) gCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAgctgc < 1:3716667/50‑1 (MQ=255) gCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCggtggt > 1:806553/1‑71 (MQ=255) gCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCggtggt > 1:1076641/1‑71 (MQ=255) cAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCTTAATCCGGCTCggtggt < 1:1554794/70‑1 (MQ=255) aaCACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGa > 1:1875520/1‑71 (MQ=255) | GCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGA > W3110S.gb/1389936‑1390068 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |