| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,683,257 | G→A | 17.1% | D190N (GAT→AAT) | folM → | dihydrofolate reductase isozyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,683,257 | 0 | G | A | 17.1% | 82.4 / 10.3 | 35 | D190N (GAT→AAT) | folM | dihydrofolate reductase isozyme |
| Reads supporting (aligned to +/- strand): ref base G (13/16); new base A (2/4); total (15/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.44e-01 | |||||||||||
GCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAG > W3110S.gb/1683188‑1683323 | gCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACatg < 1:1885887/70‑1 (MQ=255) cccGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACatgat < 1:857023/71‑1 (MQ=255) cccGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACatgat < 1:2608846/71‑1 (MQ=255) ccGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACatgatg < 1:4580393/71‑1 (MQ=255) cGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACatgatg < 1:3006128/70‑1 (MQ=255) gCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACatgatgat < 1:4427183/71‑1 (MQ=255) gCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACatgatgat > 1:331470/1‑71 (MQ=255) cAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACatgatgatg > 1:2551219/1‑71 (MQ=255) ggCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGaa > 1:4109880/1‑70 (MQ=255) gCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATAATGATGCCGAata < 1:186745/71‑1 (MQ=255) gaaagtgaaTTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAgg > 1:1552299/1‑71 (MQ=255) agagtgaaTTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATAATGATGCCGAATATCGACAACAGgc > 1:2865452/3‑71 (MQ=255) aagtgaaTTCTATTGCGCCATCGCTGATCCTGTTTAATGAACatgatga > 1:1595426/1‑49 (MQ=255) agtgaaTTCTATTGCGCCATCGCTGATCCTGTTTAATGAACatgatga > 1:369516/1‑48 (MQ=255) tgaaTTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCg > 1:715910/1‑51 (MQ=255) gaaTTCTATTGCGCCATCGCTGATCCTGTTTAATGAACataat > 1:2005845/1‑43 (MQ=255) tCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATa > 1:1058434/1‑70 (MQ=255) tCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATa > 1:1808092/1‑70 (MQ=255) cTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATaaa > 1:1777249/1‑71 (MQ=255) cTATTGCGCCATCGCTGATCCTGTTTAATGAACATAATGATGCCGAATATCGa > 1:1709589/1‑53 (MQ=255) tATTGCGCCATCGCTGATCCTGTTTAATGAACatgatga < 1:399819/39‑1 (MQ=255) tATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATaa < 1:3914995/69‑1 (MQ=255) ttGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAat < 1:4138624/45‑1 (MQ=255) ttGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATc > 1:2707383/1‑70 (MQ=255) cgcCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAata < 1:4125130/43‑1 (MQ=255) cgcCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACt < 1:3280493/70‑1 (MQ=255) cgcCATCGCTGATCCTGTTTAATGAACATAATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACtg < 1:2433246/71‑1 (MQ=255) ccATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACtgat < 1:444256/71‑1 (MQ=255) aTCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACtgatga < 1:1356547/71‑1 (MQ=255) aTCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACtgatga < 1:246031/71‑1 (MQ=255) tCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACtgatga > 1:734101/1‑70 (MQ=255) tCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGaa < 1:2038623/71‑1 (MQ=255) tCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGaa < 1:4316036/71‑1 (MQ=255) cGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATaaa > 1:3077675/1‑59 (MQ=255) gCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGaaa < 1:200390/70‑1 (MQ=255) aTCCTGTTTAATGAACATGATGATGCCGAATATCGa < 1:3835566/36‑1 (MQ=255) ccTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAAt < 1:3949745/53‑1 (MQ=255) ccTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCgcgc > 1:856424/1‑71 (MQ=255) tttAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATa < 1:1400436/46‑1 (MQ=255) tttAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTg < 1:1034170/70‑1 (MQ=255) ttAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCgcgc > 1:1616250/1‑66 (MQ=255) aTGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCgag < 1:27150/71‑1 (MQ=255) tGAACATAATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCgaga < 1:2875475/71‑1 (MQ=255) aCATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCga > 1:2383279/1‑66 (MQ=255) aCATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGagaaag < 1:2094050/71‑1 (MQ=255) aCATAATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGagaaag < 1:3429331/71‑1 (MQ=255) cATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGagaaag > 1:1603077/1‑70 (MQ=255) tgatgatgCCGAATATCGACAACAGGCGCTGAATa < 1:3339483/35‑1 (MQ=255) gatgatgCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCt < 1:2088166/57‑1 (MQ=255) | GCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAG > W3110S.gb/1683188‑1683323 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |