| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,691,217 | G→A | 10.4% | A310T (GCA→ACA) | manA → | mannose‑6‑phosphate isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,691,217 | 0 | G | A | 10.4% | 203.1 / 11.0 | 67 | A310T (GCA→ACA) | manA | mannose‑6‑phosphate isomerase |
| Reads supporting (aligned to +/- strand): ref base G (32/28); new base A (5/2); total (37/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
GGTTGCCAATGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGCTGCATGACCTTAGTGATAAAGAAA > W3110S.gb/1691150‑1691286 | ggTTGCCAATGTGAAATTCGAAGCCAAACCGGCTAACCAGTTCTTGACCCAGCCGGTGAAACAAGGTg > 1:1799532/1‑68 (MQ=255) gTTGCCAATGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAg < 1:2679431/70‑1 (MQ=255) gTTGCCAATGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAg > 1:2382201/1‑70 (MQ=255) tGCCAATGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAAc > 1:2523567/1‑71 (MQ=255) gCCAATGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAAc < 1:3450560/70‑1 (MQ=255) ccAATGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGTTGCAGAACTg < 1:2074672/71‑1 (MQ=255) ccAATGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTACAGAACTg < 1:4448876/71‑1 (MQ=255) aTGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGAc > 1:2479861/1‑71 (MQ=255) aTGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGAc > 1:3872672/1‑71 (MQ=255) aTGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTACAGAACTGGAc > 1:1013731/1‑71 (MQ=255) tgtgAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACt > 1:3861518/1‑71 (MQ=255) gAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGa > 1:3016401/1‑66 (MQ=255) aaaTTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTg > 1:2407015/1‑63 (MQ=255) aaTTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTccc > 1:980711/1‑70 (MQ=255) aTTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACt > 1:2722894/1‑60 (MQ=255) aTTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGa > 1:4425922/1‑71 (MQ=255) aTTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTACAGAACTGGACTTCCCGa > 1:4196799/1‑71 (MQ=255) ttCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAGGGTGCAGAACt > 1:4615669/1‑59 (MQ=255) tCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTccc < 1:1809272/67‑1 (MQ=255) tCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGAtt > 1:250904/1‑71 (MQ=255) aaGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTACAGAACTGGACTTCCCGATTCCa < 1:4080089/71‑1 (MQ=255) ccAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGa < 1:3376492/49‑1 (MQ=255) aaaCCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGga > 1:3838533/1‑71 (MQ=255) aaaCCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGga > 1:3971585/1‑71 (MQ=255) aCCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGgatg < 1:1958895/71‑1 (MQ=255) gCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGgatga < 1:3577056/68‑1 (MQ=255) tAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTg > 1:1835535/1‑71 (MQ=255) tAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTg > 1:4596252/1‑71 (MQ=255) aaCCAGTTGTTGACCCAGCCGGTGAAACAAGGTACAGAACt > 1:3337260/1‑41 (MQ=255) aCCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGAt < 1:2896850/52‑1 (MQ=255) aCCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGcc > 1:123502/1‑71 (MQ=255) aCCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGAATTTGcc > 1:2153624/1‑71 (MQ=255) ccAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCg > 1:2191758/1‑49 (MQ=255) cAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGAtttt > 1:2565796/1‑66 (MQ=255) cAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCtt > 1:3516018/1‑71 (MQ=255) aggtgttgACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTtc < 1:1016373/68‑1 (MQ=255) aGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTtc > 1:795124/1‑71 (MQ=255) gttgttGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTtct < 1:121080/71‑1 (MQ=255) ttgttgACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTtctc > 1:4350945/1‑71 (MQ=255) ttgttgACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTtctc < 1:1078534/71‑1 (MQ=255) tgttgACGCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCg > 1:3291134/1‑71 (MQ=255) tgttgACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCg > 1:2457907/1‑71 (MQ=255) tgttgACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCg > 1:1023311/1‑71 (MQ=255) tgttgACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCg > 1:1562076/1‑71 (MQ=255) gttgACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCg < 1:2444517/70‑1 (MQ=255) gttgACCCAGCAGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGc > 1:1764975/1‑71 (MQ=255) ttgACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGCt < 1:3795459/71‑1 (MQ=255) tgACCCAGCCGGTGAAACAAGGTACAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGCTg > 1:433069/1‑71 (MQ=255) gACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAg < 1:1636883/47‑1 (MQ=255) gACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGCTg > 1:3494408/1‑70 (MQ=255) aCCCAGCCTGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGCTGc < 1:4155546/70‑1 (MQ=255) cAGCCGGTGAAACAAGGTACAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGCTGCATGa > 1:1851924/1‑71 (MQ=255) aGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGCTGCATGAc > 1:1984754/1‑71 (MQ=255) tGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTtc < 1:3887695/53‑1 (MQ=255) aaaCAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGc < 1:259176/47‑1 (MQ=255) aaaCAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGCTg < 1:3043587/57‑1 (MQ=255) aaaCAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGCTGCATGACCTTAGTGa > 1:1547331/1‑71 (MQ=255) aaCAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGCTGCATGACCTTAGTGAt < 1:4080770/71‑1 (MQ=255) aCAAGGTGCAGAACTGGACTTCCCGATTCCAGTTGATGATTTTGCCTTCTCGCTGCATGACCTTAGTGAt > 1:2495168/1‑70 (MQ=255) cAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGCTGCATGa < 1:4113691/59‑1 (MQ=255) cAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGCTGCATGACCTTAGTGATaa < 1:3260928/71‑1 (MQ=255) aaGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGCTGCATGACCTTAGTGATaat < 1:1034608/71‑2 (MQ=255) aGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTtctc < 1:3683011/48‑1 (MQ=255) gTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGCTGCATGACCTTAGTGATAAAGaa < 1:2776334/71‑1 (MQ=255) gTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGCTGCATGACCTTAGTGATAAAGaa < 1:686237/71‑1 (MQ=255) tGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGCTGCATGACCtt < 1:3084524/58‑1 (MQ=255) tGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGCTGCATGACCTTAGTGATAAAGaaa < 1:698559/71‑1 (MQ=255) tGCAGAACTGGACTTCACGATTCCAGTGGATGATTTTGCCTTCTCGCTGCATg < 1:1041710/53‑1 (MQ=255) | GGTTGCCAATGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGCTGCATGACCTTAGTGATAAAGAAA > W3110S.gb/1691150‑1691286 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |