| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,872,074 | T→C | 8.0% | intergenic (+122/‑25) | yeaH → / → yeaI | conserved hypothetical protein/predicted diguanylate cyclase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,872,074 | 0 | T | C | 8.0% | 136.2 / 4.3 | 50 | intergenic (+122/‑25) | yeaH/yeaI | conserved hypothetical protein/predicted diguanylate cyclase |
| Reads supporting (aligned to +/- strand): ref base T (24/22); new base C (2/2); total (26/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01 | |||||||||||
ATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGTTATT > W3110S.gb/1872005‑1872133 | aTCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGtt > 1:2872004/1‑71 (MQ=255) ctcATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTc > 1:4521287/1‑70 (MQ=255) aTTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGCTCCa < 1:4512818/69‑1 (MQ=255) tttATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGa > 1:4225803/1‑70 (MQ=255) ttattaCATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGaa > 1:438578/1‑70 (MQ=255) tattaCATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGaaaa > 1:4013548/1‑71 (MQ=255) ttaCATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAAc < 1:1141602/70‑1 (MQ=255) catacatTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAAt < 1:2875700/70‑1 (MQ=255) catacatTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAAt < 1:4495134/70‑1 (MQ=255) tacatTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGtt > 1:2920532/1‑56 (MQ=255) tacatTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGtt > 1:1629394/1‑56 (MQ=255) tacatTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCAt < 1:3734994/71‑1 (MQ=255) acatTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCAtt > 1:2035174/1‑71 (MQ=255) acatTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCAtt > 1:2040400/1‑71 (MQ=255) acatTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCAtt > 1:1209206/1‑71 (MQ=255) atTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCg > 1:688489/1‑71 (MQ=255) atTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGCTCCAGAAAACAATCATTCg > 1:2389113/1‑71 (MQ=255) aGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCgg > 1:211224/1‑69 (MQ=255) aGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGa > 1:2464/1‑70 (MQ=255) aGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGa < 1:2660987/70‑1 (MQ=255) tACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTcc > 1:3549348/1‑48 (MQ=255) aCAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACaa < 1:1965428/56‑1 (MQ=255) aCAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGaaaaa < 1:1994461/69‑1 (MQ=255) aCAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAAt > 1:70335/1‑70 (MQ=255) aCAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAAt < 1:2283944/70‑1 (MQ=255) tCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACa < 1:3408991/51‑1 (MQ=255) cGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGAtt > 1:3021272/1‑69 (MQ=255) cTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAAt < 1:4014380/50‑1 (MQ=255) tAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTc < 1:257536/69‑1 (MQ=255) aCAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGaaa > 1:434156/1‑49 (MQ=255) aGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCAt < 1:1622517/40‑1 (MQ=255) aGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTc < 1:3316630/71‑1 (MQ=255) gCATAACCTTTACATAATTTAGTTCCAGAAAACaa < 1:2365986/35‑1 (MQ=255) aTAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCg > 1:1316150/1‑40 (MQ=255) aTAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGaaaaa > 1:960543/1‑46 (MQ=255) tAACCTTTACATAATTTAGTTCCAGAAAACAATCACTCGGaaaaa > 1:3219450/1‑45 (MQ=255) aCCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGAtt > 1:3606133/1‑48 (MQ=255) aCCTTTACATAATTTAGCTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTa > 1:3679668/1‑61 (MQ=255) ccTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTc < 1:393305/64‑1 (MQ=255) tttACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCa < 1:784663/64‑1 (MQ=255) tttACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCa < 1:544254/64‑1 (MQ=255) tttACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGt > 1:1578016/1‑70 (MQ=255) tACATAATTTAGCTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGtt < 1:1103721/69‑1 (MQ=255) aCATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGAtt > 1:1233738/1‑42 (MQ=255) aCATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTcc > 1:3349038/1‑60 (MQ=255) aCATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCa > 1:1092428/1‑61 (MQ=255) aCATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGtt < 1:396207/68‑1 (MQ=255) aCATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGTTa < 1:27880/69‑1 (MQ=255) aTAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAac > 1:887843/1‑48 (MQ=255) aTAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGt < 1:1448972/52‑1 (MQ=255) aTAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGTTAtt < 1:4267523/69‑1 (MQ=255) tAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCaa > 1:4596433/1‑46 (MQ=255) tAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTc < 1:2164122/56‑1 (MQ=255) | ATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGTTATT > W3110S.gb/1872005‑1872133 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |